Basic Information
ID: ALA3887107
Type: Binding
Description: Inhibition Assay: 11 data point. Cdc7 kinase assays were carried out in 25 mM HEPES, pH 7.5, 1 mM DTT, 10 mM MgCl2, 100 μM Na3VO4, and 0.075 mg/ml Triton X-100 using 12 ng baculovirus-expressed Cdc7/Dbf4 and 2 μM Jerini peptide substrate A-A11 (biotin-C6linker-TPSDSLIYDDGLS) (SEQ ID NO:5). Reactions were initiated with λ-[33P]-ATP (1 μM, 20 mCi/μmol) and quenched after 1 hour with 5 volumes of stop buffer (50 mM EDTA, 2 M NaCl). The reactions were incubated for 30 minutes on streptavidin-coated plates, washed, and quantified using a TopCount scintillation plate reader (Packard). IC50 values are determined via non-linear regression fitting of the data. Ki values are generated assuming ATP-competitive (equilibrium) inhibition and using the experimentally determined apparent ATP Km of Cdc7 (0.7 μM).
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3887107
Ki
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 290.33 | 2.45 | 2.45 |
| 293.31 | 3.01 | 3.01 |
| 432.49 | 4.18 | 4.18 |
| 409.50 | 4.18 | 4.18 |
| 351.41 | 4.54 | 4.54 |
| 366.43 | 4.12 | 4.12 |
| 383.43 | 4.98 | 4.98 |
| 351.41 | 4.54 | 4.54 |
| 379.47 | 5.15 | 5.15 |
| 384.42 | 4.26 | 4.26 |
| 304.36 | 2.76 | 2.76 |
| 394.44 | 4.12 | 4.12 |
| 380.46 | 4.43 | 4.43 |
| 412.43 | 4.26 | 4.26 |
| 330.40 | 3.33 | 3.33 |
| 372.46 | 4.18 | 4.18 |
| 505.00 | 5.20 | 5.20 |
| 454.97 | 6.01 | 6.01 |
| 372.48 | 4.18 | 4.18 |
| 346.39 | 2.57 | 2.57 |
| 333.35 | 2.66 | 2.66 |
| 362.39 | 2.45 | 2.45 |
| 388.48 | 3.36 | 3.36 |
| 403.49 | 3.15 | 3.15 |
| 398.45 | 4.62 | 4.62 |
| 470.54 | 5.79 | 5.79 |
| 361.41 | 3.01 | 3.01 |
| 405.47 | 4.35 | 4.35 |
| 358.43 | 3.99 | 3.99 |
| 414.90 | 5.14 | 5.14 |
| 382.45 | 2.63 | 2.63 |
| 346.44 | 4.08 | 4.08 |
| 515.58 | 6.19 | 6.19 |
| 261.29 | 2.81 | 2.81 |
| 370.42 | 2.85 | 2.85 |
| 403.49 | 2.61 | 2.61 |
| 452.42 | 5.28 | 5.28 |
| 401.47 | 2.95 | 2.95 |
| 451.53 | 3.96 | 3.96 |
| 358.41 | 3.00 | 3.00 |
| 357.44 | 4.60 | 4.60 |
| 416.44 | 4.76 | 4.76 |
| 422.51 | 3.51 | 3.51 |
| 344.42 | 3.69 | 3.69 |
| 430.47 | 1.24 | 1.24 |
| 386.50 | 4.57 | 4.57 |
| 375.44 | 3.16 | 3.16 |
| 483.96 | 5.62 | 5.62 |
| 386.50 | 4.86 | 4.86 |
| 380.46 | 4.04 | 4.04 |
| 429.53 | 3.73 | 3.73 |
| 454.97 | 6.01 | 6.01 |
| 373.46 | 2.62 | 2.62 |
| 355.41 | 3.27 | 3.27 |
| 452.49 | 5.14 | 5.14 |
| 505.00 | 5.20 | 5.20 |
| 538.61 | 6.16 | 6.16 |
| 372.48 | 4.50 | 4.50 |
| 344.21 | 4.18 | 4.18 |
| 468.95 | 5.65 | 5.65 |
| 377.42 | 4.05 | 4.05 |
| 463.55 | 4.83 | 4.83 |
| 275.32 | 2.87 | 2.87 |
| 360.42 | 2.71 | 2.71 |
| 470.56 | 4.55 | 4.55 |
| 410.48 | 4.49 | 4.49 |
| 380.45 | 4.48 | 4.48 |
| 506.59 | 5.34 | 5.34 |