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Fluorescence Polarization Assays: In another embodiment, Fluorescence Polarization ("FP") based assays may be used to detect and monitor ligand bindin...

Basic Information

ID: ALA3887148

Type: Binding

Description: Fluorescence Polarization Assays: In another embodiment, Fluorescence Polarization ("FP") based assays may be used to detect and monitor ligand binding. Fluorescence polarization is a versatile laboratory technique for measuring equilibrium binding, nucleic acid hybridization, and enzymatic activity. Fluorescence polarization assays are homogeneous in that they do not require a separation step such as centrifugation, filtration, chromatography, precipitation, or electrophoresis. These assays are done in real time, directly in solution and do not require an immobilized phase. Polarization values can be measured repeatedly and after the addition of reagents since measuring the polarization is rapid and does not destroy the sample. Generally, this technique can be used to measure polarization values of fluorophores from low picomolar to micromolar levels.

Format: BAO_0000357

Target:  Taste receptor type 2 member 14(ALA3309105)

Document:  ALA3886188

BindingDB Source:  7695

Activity Charts

Compound Summary

Parent Molecular WeightALogPPolar Surface Area
415.904.434.43
461.035.755.75
442.584.794.79
450.004.904.90
365.42-0.71-0.71
377.401.491.49
389.390.770.77
377.401.491.49
494.455.015.01
391.500.940.94
403.420.810.81
381.453.783.78
450.52-0.02-0.02
458.541.221.22
417.534.164.16
411.483.783.78
397.504.094.09
397.453.483.48
423.493.883.88
441.513.793.79
399.443.923.92
425.514.094.09
415.904.434.43
363.442.992.99
395.484.084.08
401.443.383.38
401.534.084.08
398.441.531.53
429.494.164.16
415.904.434.43
552.494.784.78
401.443.383.38
427.483.493.49
424.615.655.65
409.463.763.76
471.533.803.80
425.513.833.83
415.564.394.39
435.523.923.92
425.463.503.50
413.474.224.22
420.513.493.49
399.443.923.92
322.353.343.34
399.443.923.92
419.524.054.05
431.554.094.09
351.430.850.85
406.483.303.30
395.484.084.08
471.533.803.80
451.544.664.66
471.533.803.80
399.443.923.92
472.614.214.21
353.422.382.38
444.553.733.73
401.443.383.38
445.924.444.44
441.513.793.79
427.483.493.49
429.473.923.92
419.524.224.22
441.513.793.79
445.924.444.44
371.413.373.37
399.443.923.92
359.411.351.35
436.493.663.66
415.473.773.77
397.821.831.83
447.464.064.06
353.422.382.38
403.420.810.81
405.493.913.91
459.503.933.93
427.483.493.49
425.471.601.60
383.452.392.39
415.904.434.43
435.513.273.27
337.41-0.09-0.09
411.483.783.78
313.422.692.69
411.483.783.78
348.431.371.37
423.493.733.73
412.473.183.18
406.463.653.65
363.440.440.44
415.443.623.62
399.443.923.92
423.493.733.73
349.412.602.60
370.431.041.04
464.035.215.21
406.463.653.65
255.25-0.88-0.88
395.484.084.08
425.514.094.09
411.523.973.97
436.492.022.02
462.543.873.87
415.443.623.62
425.514.354.35
441.513.793.79
411.483.783.78
362.461.131.13
376.403.483.48
445.036.056.05
427.554.504.50
411.483.783.78
426.543.953.95
459.503.933.93
409.514.344.34
411.483.783.78