Assay Report Card

Basic Information

ID: ALA3887160

Type: Binding

Description: Inhibition Assay: Test compounds dissolved and diluted in DMSO (2 μl) were added to a reaction mixture comprising 10 μl of 5× Reaction Buffer (40 mM MOPS pH 7.0, 5 mM EDTA), 10 μl of recombinant human PIM2 solution (4 ng PIM-2 dissolved in dilution buffer (20 mM MOPS pH 7.0; EDTA 1 mM; 5% Glycerol; 0.01% Brij 35; 0.1%; 0.1% 2-mercaptoethanol; 1 mg/ml BSA)) and 8 ul of water. Reactions were initiated by the addition of 10 ul of ATP Solution (49% (15 mM MgCl2; 75 uM ATP) 1% ([γ-33P]ATP: Stock 1 mCi/100 μl; 3000 Ci/mmol (Perkin Elmer)) and 10 ul of substrate peptide solution (RSRSSYPAGT (SEQ ID NO.: 6), dissolved in water at a concentration of 1 mM), Reactions were maintained for 10 min at 30° C. The reactions were quenched with 100 ul of 0.75% Phosphoric acid, then transferred to and filtered through a Phosphocellulose filter plate (Millipore, MSPH-N6B-50). After washing each well 4 times with 0.75% Phosphoric acid, scintillation fluid (20 uL) was added to each well and the resid

Activity Charts

Compound Summary

Parent Molecular WeightALogPPolar Surface Area
473.542.482.48
446.431.561.56
450.461.511.51
419.451.841.84
405.421.451.45
453.551.851.85
461.490.290.29
434.461.411.41
472.433.073.07
416.401.741.74
454.493.213.21
488.561.241.24
555.601.721.72
423.412.382.38
418.462.112.11
402.371.541.54
541.581.001.00
418.462.362.36
503.520.960.96
439.382.012.01
473.542.122.12
398.430.490.49
495.54-0.54-0.54
404.431.801.80
450.46-0.10-0.10
429.441.831.83
433.482.152.15
483.332.282.28
396.441.551.55
461.362.572.57
418.462.442.44
444.412.332.33
418.462.082.08
476.392.652.65
448.442.142.14
479.551.521.52
557.561.471.47
471.490.470.47
432.440.950.95
426.391.771.77
438.882.712.71
422.45-0.09-0.09
419.451.761.76
422.371.411.41
430.472.372.37
432.401.551.55
489.541.361.36
541.580.990.99
418.380.940.94
433.482.152.15
416.451.981.98
471.402.752.75
396.441.551.55
447.501.551.55
479.551.521.52
419.381.631.63
442.842.282.28
448.441.511.51
434.461.411.41
517.621.321.32
351.330.060.06
455.883.453.45
370.370.630.63
434.462.062.06
415.421.531.53
408.430.530.53
468.500.890.89
521.631.811.81
458.402.512.51
456.471.681.68
470.501.991.99
432.492.752.75
438.882.172.17
442.842.282.28
418.411.871.87
499.530.970.97
394.400.220.22
413.341.951.95
384.400.330.33
456.872.312.31
442.442.462.46
461.51-0.65-0.65
473.501.621.62
446.523.083.08
422.421.941.94
406.411.201.20
455.482.282.28
554.612.322.32
401.391.221.22
440.412.082.08
419.381.631.63
436.480.540.54
446.252.982.98
457.372.152.15
412.451.111.11
456.872.592.59
411.812.322.32
410.461.861.86
483.332.812.81
470.501.991.99
474.402.392.39
461.491.191.19
517.621.181.18
392.380.850.85
447.501.591.59
484.521.071.07
415.421.361.36
432.492.612.61
536.002.702.70
503.572.202.20
459.302.802.80
398.431.401.40
554.612.322.32
406.410.590.59
447.48-1.04-1.04
415.421.361.36
438.882.172.17
476.500.720.72
476.392.652.65
420.390.770.77
422.422.192.19
442.842.282.28
444.461.381.38
413.341.951.95
434.421.191.19
434.460.660.66
422.422.192.19
401.391.491.49
501.570.680.68
440.852.282.28
418.44-0.89-0.89
490.473.103.10
397.420.950.95
452.521.731.73
416.451.981.98
455.842.532.53
533.522.062.06
457.461.071.07
434.421.121.12
473.323.363.36
461.491.191.19
404.431.521.52
422.421.651.65
450.461.511.51
429.802.462.46
476.392.652.65
503.520.630.63
478.562.122.12
448.492.522.52
462.471.651.65
561.632.062.06
474.550.760.76
442.842.282.28
450.500.930.93
463.51-0.19-0.19
419.451.841.84
441.842.332.33
437.511.171.17
407.441.371.37
469.512.132.13
394.400.220.22
476.392.652.65
376.330.330.33
442.441.811.81
435.832.802.80
422.421.941.94
446.252.982.98
524.590.050.05
413.44-1.01-1.01
459.391.991.99
441.461.311.31
500.511.981.98
459.302.802.80
476.392.652.65
436.502.212.21
438.882.452.45
454.491.561.56
454.882.152.15
596.694.064.06
420.431.501.50
394.441.481.48
405.380.530.53
409.382.122.12
358.36-0.06-0.06
459.302.802.80
434.421.121.12
473.542.482.48
406.43-0.66-0.66
418.462.112.11
536.640.570.57
504.471.471.47
510.601.951.95
441.500.400.40
398.430.720.72
432.492.692.69
469.490.140.14
466.513.163.16
384.400.110.11
426.391.771.77
416.451.981.98
405.420.910.91
474.531.511.51
428.822.412.41
405.421.451.45
501.501.371.37
470.502.242.24
459.302.802.80
393.410.760.76
442.842.282.28
498.541.581.58
441.842.332.33
512.531.061.06
503.571.701.70
422.422.192.19
448.441.781.78
489.322.332.33
476.392.652.65
422.410.860.86
521.631.811.81
502.581.281.28
522.611.201.20
474.512.012.01
555.601.721.72
434.461.331.33
407.391.681.68
456.872.842.84
443.471.981.98
459.302.802.80
447.501.551.55
387.762.032.03
462.471.821.82
394.400.220.22
434.421.221.22
432.441.811.81
502.581.631.63
472.460.790.79
451.541.331.33
370.370.080.08
452.411.261.26
481.272.312.31
395.351.811.81
401.391.651.65
410.822.932.93
442.842.282.28
440.412.332.33
353.350.200.20
418.461.911.91
419.451.301.30
490.531.021.02
487.292.392.39
438.882.712.71
407.391.161.16
450.892.632.63
468.500.890.89
395.351.811.81
456.471.681.68
445.492.152.15
447.482.102.10
409.842.882.88
367.370.230.23
421.391.881.88
456.471.681.68
500.591.291.29
394.371.761.76
394.400.220.22
442.842.282.28
432.492.612.61
420.361.681.68
468.521.541.54
447.460.850.85
386.440.430.43
459.302.802.80
450.420.890.89
445.441.021.02
492.853.163.16
488.561.241.24
438.882.452.45
497.534.214.21
562.621.451.45
479.551.521.52
445.441.011.01
415.421.531.53
458.402.512.51
395.380.790.79
412.451.111.11
422.421.651.65
514.471.621.62
484.521.071.07
470.502.242.24
469.302.252.25
456.872.592.59
522.611.201.20
476.502.212.21
445.441.021.02
438.522.752.75
409.382.122.12
386.772.282.28
516.611.631.63
398.430.490.49
397.441.861.86
562.621.451.45
514.471.621.62
481.272.312.31
422.422.192.19
410.421.191.19
458.402.512.51
426.391.771.77
438.882.452.45
429.802.462.46
434.460.970.97
453.510.180.18
469.302.252.25
483.332.282.28
416.451.441.44
425.841.541.54
470.501.701.70
416.402.152.15
454.882.022.02
394.400.220.22
397.440.070.07
419.451.761.76
461.362.572.57
391.390.890.89
419.451.761.76
425.842.112.11
428.822.412.41
421.421.991.99
415.421.361.36
394.401.641.64
489.541.631.63
472.433.073.07
438.882.452.45
365.360.370.37
461.491.471.47
479.813.343.34
474.402.392.39
450.461.511.51
531.621.581.58
430.472.372.37
467.531.481.48
438.882.452.45
410.462.112.11
457.502.252.25
407.391.161.16
450.461.511.51
402.371.541.54
477.531.431.43
475.511.331.33
509.622.552.55
485.510.860.86
410.482.262.26
448.491.441.44
421.471.541.54
473.542.092.09
489.541.321.32
561.632.062.06
450.892.632.63
556.572.082.08
521.631.811.81
470.501.931.93
532.532.672.67
418.462.112.11
458.533.223.22
456.471.681.68
402.371.541.54
459.512.092.09
432.492.832.83
555.601.431.43
426.391.771.77
429.802.462.46
406.411.201.20
479.551.371.37
466.550.320.32
425.842.112.11
450.461.511.51
400.40-0.29-0.29
434.462.062.06
482.531.461.46
435.422.182.18
467.872.402.40
428.411.611.61
438.882.712.71
476.52-1.19-1.19
411.811.671.67
436.822.202.20
522.611.201.20
502.601.541.54
401.391.491.49
419.401.871.87
450.500.930.93
402.421.571.57
407.391.681.68
443.431.861.86
410.440.150.15
422.450.840.84
476.392.652.65
398.430.720.72
412.41-0.59-0.59
421.391.881.88
489.541.321.32
444.461.191.19
471.490.470.47
436.401.661.66
432.441.431.43
478.562.122.12
380.411.031.03
451.441.731.73
405.420.910.91
428.412.222.22
398.430.490.49
392.380.280.28
476.392.652.65
496.551.851.85
446.502.712.71
446.252.982.98
453.422.092.09
420.431.741.74
404.431.801.80
470.501.701.70
551.631.721.72
410.461.861.86
462.402.472.47
503.590.930.93
483.332.282.28
514.471.621.62
462.471.651.65
404.431.801.80
436.502.212.21
572.602.462.46
516.631.931.93
461.51-0.28-0.28
433.481.561.56
418.462.052.05
448.492.282.28
470.501.081.08
442.842.282.28
426.391.771.77
405.421.451.45
450.500.930.93
419.451.221.22
426.481.501.50
455.842.532.53
516.302.102.10
429.802.462.46
469.512.132.13
431.461.761.76
406.411.201.20
441.452.412.41
463.352.832.83
485.510.860.86
459.391.901.90
473.542.132.13
478.561.981.98
461.531.981.98
426.391.771.77
396.461.171.17
445.272.932.93
401.391.491.49
483.332.812.81
514.471.621.62
448.492.282.28
438.882.712.71
448.441.261.26
394.401.391.39
434.461.891.89
411.812.322.32
446.431.751.75
537.63-0.03-0.03
393.410.620.62
440.412.332.33
419.401.871.87
454.493.213.21
402.421.401.40
407.391.681.68
434.421.191.19
501.602.722.72
478.562.122.12
446.252.982.98
391.391.461.46
418.462.442.44
418.462.112.11
411.812.322.32
442.842.282.28
474.402.392.39
419.451.761.76
433.482.232.23
516.631.781.78
465.542.222.22
478.823.293.29
448.441.121.12
523.600.660.66
438.491.341.34
449.862.022.02
431.461.621.62
446.471.991.99
492.853.163.16
453.510.400.40
454.882.022.02
408.421.701.70
573.591.861.86
430.432.462.46
574.653.003.00
405.421.191.19
469.302.252.25
453.511.131.13
418.462.232.23
434.462.062.06
540.434.094.09
422.422.192.19
472.433.073.07
447.432.062.06
416.451.981.98
492.303.463.46
418.462.362.36
436.452.502.50
438.882.712.71
454.493.213.21
456.872.842.84
438.882.712.71
434.462.062.06
483.332.812.81
454.493.213.21
406.411.411.41
430.472.372.37
438.882.712.71
448.491.441.44
432.440.950.95
418.462.082.08
410.441.401.40
410.482.262.26
418.462.442.44
405.421.451.45