Assay Report Card

Basic Information

ID: ALA3887224

Type: Binding

Description: Enzyme Assay: The test uses a lucifeurerin/luciferase system to measure the concentration of ATP and its consumption during the enzymatic reaction. The test is performed in 96-well format (Corning/Costar 96 black flat-bottomed half-wells plate, ref. 3694) in a total volume of 30 μl. To 1 μl of inhibitor in 100% DMSO are added (final concentrations) 50 μM of the substrate PIP2 ((L-α-phosphatidyl-D-myoinositol 4,5-bisphosphate, Echelon 117P-4516-0500), 2 μM of ATP and 1.7 μg/ml of PI3Kα, (p110α/p85α, Invitrogen PV4788) in a buffer of Tris/HCl 50 mM pH 7.5, EGTA 1 mM, MgCl2 10 mM, Chaps 0.03%, 1 mM DTT). After 90 minutes, the reaction is quenched by adding 20 μl/well of KinaseGlo reagent (Promega V6713). After 10 minutes in the dark, the luminescence is read on the PHERAStar microplate reader (reading at 0.8 sec/well).

Organism: Homo sapiens

Activity Charts

Bioactivity

Activity Types for Assay ALA3887224

IC50

Total(254)

Compound Summary

Parent Molecular Weight	ALogP	Polar Surface Area
573.65	4.19	4.19
573.65	4.19	4.19
634.71	4.02	4.02
564.62	3.66	3.66
442.52	2.11	2.11
494.60	3.29	3.29
646.72	5.10	5.10
586.70	4.25	4.25
629.77	3.79	3.79
547.62	4.08	4.08
539.57	3.77	3.77
581.61	3.54	3.54
674.78	4.94	4.94
609.70	3.42	3.42
589.67	4.94	4.94
658.73	4.87	4.87
672.76	4.69	4.69
596.63	5.25	5.25
567.67	3.16	3.16
629.61	5.34	5.34
595.72	3.75	3.75
582.68	3.83	3.83
579.63	4.19	4.19
561.62	4.32	4.32
481.55	3.32	3.32
647.76	3.93	3.93
696.85	4.95	4.95
551.58	3.92	3.92
552.61	3.52	3.52
658.78	4.68	4.68
660.75	4.55	4.55
575.65	4.63	4.63
482.59	3.14	3.14
640.79	4.54	4.54
628.73	3.75	3.75
547.59	3.93	3.93
543.63	4.10	4.10
561.62	4.24	4.24
549.61	4.18	4.18
512.59	3.42	3.42
702.79	4.82	4.82
684.84	4.81	4.81
579.63	4.19	4.19
660.75	4.55	4.55
561.64	4.05	4.05
547.59	3.93	3.93
582.67	4.66	4.66
550.66	3.44	3.44
646.72	4.16	4.16
524.62	2.65	2.65
703.78	4.21	4.21
658.74	4.30	4.30
479.58	3.49	3.49
529.60	3.79	3.79
587.68	4.50	4.50
545.64	4.35	4.35
564.62	3.88	3.88
486.55	2.89	2.89
646.72	4.16	4.16
575.65	4.63	4.63
617.71	4.21	4.21
630.73	4.92	4.92
605.68	3.78	3.78
511.58	2.68	2.68
540.60	2.80	2.80
468.56	2.75	2.75
596.64	4.93	4.93
512.59	3.24	3.24
592.63	3.73	3.73
543.63	4.18	4.18
554.58	3.48	3.48
591.64	4.33	4.33
554.55	3.82	3.82
723.83	3.40	3.40
542.61	2.79	2.79
521.65	4.05	4.05
466.54	2.51	2.51
559.63	3.80	3.80
538.61	2.74	2.74
591.64	4.33	4.33
610.64	5.81	5.81
542.61	3.05	3.05
535.61	3.94	3.94
594.71	4.00	4.00
515.64	2.91	2.91
604.69	4.39	4.39
470.53	2.30	2.30
575.65	4.63	4.63
546.63	3.52	3.52
605.67	4.01	4.01
495.58	3.71	3.71
563.62	3.40	3.40
484.53	2.64	2.64
561.62	4.24	4.24
561.62	4.32	4.32
639.73	4.03	4.03
560.66	4.05	4.05
440.50	1.98	1.98
573.65	4.19	4.19
600.65	4.80	4.80
579.61	4.46	4.46
511.58	2.69	2.69
607.64	4.07	4.07
510.60	2.26	2.26
626.66	4.87	4.87
514.60	3.67	3.67
659.77	4.07	4.07
603.68	4.24	4.24
559.63	3.80	3.80
629.77	3.79	3.79
574.64	3.59	3.59
658.80	4.28	4.28
547.59	3.93	3.93
527.62	3.32	3.32
572.67	4.20	4.20
603.68	3.82	3.82
561.62	4.32	4.32
564.62	3.66	3.66
525.61	3.07	3.07
486.55	2.89	2.89
500.58	3.28	3.28
512.59	3.42	3.42
524.60	3.57	3.57
561.62	4.32	4.32
559.60	4.07	4.07
644.75	5.31	5.31
647.71	3.55	3.55
572.67	4.80	4.80
543.63	4.18	4.18
529.60	3.79	3.79
574.65	2.66	2.66
616.72	2.84	2.84
629.72	4.35	4.35
455.52	2.79	2.79
561.64	4.05	4.05
495.58	2.68	2.68
563.63	4.49	4.49
549.61	4.18	4.18
578.62	5.07	5.07
608.65	4.69	4.69
561.62	4.32	4.32
612.74	4.78	4.78
531.62	4.04	4.04
595.63	3.93	3.93
498.56	3.03	3.03
542.61	3.05	3.05
657.77	4.29	4.29
559.63	3.80	3.80
544.62	3.58	3.58
545.64	4.35	4.35
617.71	3.27	3.27
561.62	4.24	4.24
645.76	4.15	4.15
561.62	4.32	4.32
552.61	3.52	3.52
674.78	4.80	4.80
528.59	2.66	2.66
680.73	5.56	5.56
556.64	3.18	3.18
563.63	4.49	4.49
543.63	4.10	4.10
592.63	3.73	3.73
539.63	3.46	3.46
564.65	3.01	3.01
544.62	3.58	3.58
522.59	3.45	3.45
575.67	4.36	4.36
551.60	3.26	3.26
608.65	5.08	5.08
574.64	3.59	3.59
602.70	3.68	3.68
596.63	4.55	4.55
610.66	5.48	5.48
628.63	5.95	5.95
500.58	3.28	3.28
561.64	4.62	4.62
524.62	2.91	2.91
550.60	3.57	3.57
517.56	2.68	2.68
628.73	4.02	4.02
466.54	2.51	2.51
587.66	4.69	4.69
543.63	4.18	4.18
654.77	4.55	4.55
578.65	4.79	4.79
440.50	2.02	2.02
529.57	2.82	2.82
639.80	5.77	5.77
592.63	3.73	3.73
524.62	2.65	2.65
524.60	3.57	3.57
633.73	4.62	4.62
568.65	3.58	3.58
672.76	4.69	4.69
621.67	3.96	3.96
484.53	2.64	2.64
658.74	4.30	4.30
547.62	4.08	4.08
674.78	4.94	4.94
608.65	5.39	5.39
574.64	3.59	3.59
561.62	4.32	4.32
484.53	2.64	2.64
529.60	3.79	3.79
563.63	4.49	4.49
722.84	4.01	4.01
499.57	2.54	2.54
646.72	4.16	4.16
591.64	4.33	4.33
566.61	4.92	4.92
543.60	3.21	3.21
510.64	3.78	3.78
512.59	3.42	3.42
646.72	4.16	4.16
516.57	2.52	2.52
529.57	2.82	2.82
529.60	3.79	3.79
608.65	5.08	5.08
529.57	2.82	2.82
615.67	4.22	4.22
370.41	0.86	0.86
662.77	4.80	4.80
498.56	3.03	3.03
559.63	3.80	3.80
498.56	3.03	3.03
670.82	4.42	4.42
549.61	4.18	4.18
563.70	3.36	3.36
546.63	3.52	3.52
579.63	4.19	4.19
662.77	4.80	4.80
548.62	4.78	4.78
635.70	4.35	4.35
557.66	4.49	4.49
397.48	2.20	2.20
424.51	2.12	2.12
573.63	4.38	4.38
579.63	4.19	4.19
496.61	3.53	3.53
693.80	4.16	4.16
652.70	5.25	5.25
577.66	4.88	4.88
589.65	3.94	3.94
586.63	4.19	4.19
565.68	2.74	2.74
592.63	3.73	3.73
546.63	3.52	3.52
543.60	3.21	3.21
646.72	4.16	4.16
660.75	4.55	4.55