Basic Information
ID: ALA3887240
Type: Binding
Description: Fluorometric Assay: All measurements were performed with a BioAssay Reader HTS-7000Plus for microplates (Perkin Elmer) at 30 C. QC activity was evaluated fluorometrically using H-Gln-beta NA. The samples consisted of 0.2 mM fluorogenic substrate, 0.25 U pyroglutamyl aminopeptidase (Unizyme, Horsholm, Denmark) in 0.2 M Tris/HCl, pH 8.0 containing 20 mM EDTA and an appropriately diluted aliquot of QC in a final volume of 250 ul. Excitation/emission wavelengths were 320/410 nm. The assay reactions were initiated by addition of glutaminyl cyclase. QC activity was determined from a standard curve of beta -naphthylamine under assay conditions. One unit is defined as the amount of QC catalyzing the formation of 1 umol pGlu-beta NA from H-Gln-beta NA per minute under the described conditions.In a second fluorometric assay, QC was activity determined using H-Gln-AMC as substrate. Reactions were carried out at 30 C. utilizing the NOVOStar reader for microplates (BMG labtechnologies).
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3887240
Ki
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 232.27 | 2.33 | 2.33 |
| 246.29 | 2.72 | 2.72 |
| 330.46 | 5.07 | 5.07 |
| 258.31 | 2.89 | 2.89 |
| 308.37 | 3.91 | 3.91 |
| 358.43 | 5.06 | 5.06 |
| 322.39 | 3.95 | 3.95 |
| 336.42 | 4.34 | 4.34 |
| 291.31 | 3.23 | 3.23 |
| 342.81 | 4.56 | 4.56 |
| 326.36 | 4.04 | 4.04 |
| 295.28 | 3.50 | 3.50 |
| 218.24 | 1.90 | 1.90 |
| 309.35 | 3.30 | 3.30 |
| 378.50 | 5.45 | 5.45 |
| 364.47 | 5.20 | 5.20 |
| 309.35 | 3.30 | 3.30 |
| 337.34 | 3.37 | 3.37 |
| 398.32 | 4.60 | 4.60 |
| 311.73 | 4.01 | 4.01 |
| 309.30 | 3.36 | 3.36 |
| 350.45 | 4.83 | 4.83 |
| 398.44 | 3.93 | 3.93 |
| 305.34 | 3.53 | 3.53 |
| 362.34 | 4.32 | 4.32 |