Basic Information
ID: ALA3887241
Type: Binding
Description: Biological Assay: Human TASK-1 channels were expressed in Xenopus oocytes. For this purpose, oocytes were isolated from Xenopus laevis and defoliculated. Subsequently, TASK-1-encoding RNA synthesized in vitro was injected into oocytes. After two days of TASK-1 protein expression, TASK-1 currents were measured by two-microelectrode voltage clamp. Data were acquired and analyzed using a TEC-10cx amplifier (NPI Electronic, Tamm, Germany) connected to an ITC-16 interface (Instrutech Corp., Long Island, USA) and Pulse software (HEKA Elektronik, Lambrecht, Germany). Oocytes were clamped to −90 mV and TASK-1 mediated currents were measured during 500 ms voltage pulses to 40 mV. Oocytes were continuously superfused with ND96 buffer containing: NaCl 96 mM, KCl 2 mM, CaCl2 1.8 mM, MgCl2 1 mM, HEPES 5 mM (pH adjusted to 7.4 with NaOH). All experiments were performed at room temperature.
Activity Charts
Bioactivity
Activity Types for Assay ALA3887241
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 456.46 | 3.74 | 3.74 |
| 411.91 | 5.25 | 5.25 |
| 436.38 | 4.20 | 4.20 |
| 414.43 | 4.23 | 4.23 |
| 399.42 | 4.31 | 4.31 |
| 434.85 | 4.57 | 4.57 |
| 410.47 | 4.53 | 4.53 |
| 385.39 | 3.92 | 3.92 |
| 429.47 | 5.08 | 5.08 |
| 451.40 | 4.68 | 4.68 |
| 388.45 | 4.07 | 4.07 |
| 415.42 | 3.93 | 3.93 |
| 392.41 | 3.65 | 3.65 |
| 432.47 | 4.43 | 4.43 |
| 424.43 | 4.39 | 4.39 |
| 407.42 | 3.45 | 3.45 |
| 441.51 | 4.94 | 4.94 |
| 447.28 | 3.94 | 3.94 |
| 408.41 | 2.63 | 2.63 |
| 381.43 | 4.34 | 4.34 |
| 458.47 | 3.68 | 3.68 |
| 395.89 | 4.17 | 4.17 |
| 385.39 | 3.92 | 3.92 |
| 448.47 | 3.67 | 3.67 |
| 393.40 | 3.05 | 3.05 |
| 452.51 | 3.98 | 3.98 |
| 436.38 | 4.20 | 4.20 |
| 393.40 | 3.05 | 3.05 |
| 401.84 | 4.43 | 4.43 |
| 411.43 | 4.62 | 4.62 |
| 532.57 | 4.35 | 4.35 |
| 434.49 | 4.68 | 4.68 |
| 407.46 | 4.73 | 4.73 |
| 448.47 | 3.67 | 3.67 |
| 462.50 | 4.06 | 4.06 |
| 397.43 | 3.79 | 3.79 |
| 434.85 | 4.57 | 4.57 |
| 418.45 | 4.04 | 4.04 |
| 417.48 | 3.76 | 3.76 |
| 420.46 | 4.29 | 4.29 |
| 418.45 | 4.36 | 4.36 |
| 417.41 | 4.66 | 4.66 |
| 447.28 | 3.94 | 3.94 |
| 385.39 | 3.92 | 3.92 |
| 417.41 | 4.66 | 4.66 |
| 422.44 | 3.01 | 3.01 |
| 406.44 | 4.04 | 4.04 |
| 415.42 | 3.93 | 3.93 |
| 452.51 | 4.25 | 4.25 |
| 447.43 | 5.10 | 5.10 |
| 532.57 | 4.35 | 4.35 |
| 420.46 | 4.43 | 4.43 |
| 473.46 | 4.48 | 4.48 |
| 435.40 | 4.80 | 4.80 |
| 407.87 | 4.50 | 4.50 |
| 458.47 | 3.68 | 3.68 |
| 447.28 | 3.94 | 3.94 |
| 410.40 | 3.79 | 3.79 |
| 418.39 | 4.06 | 4.06 |
| 425.51 | 4.86 | 4.86 |
| 446.50 | 4.82 | 4.82 |
| 411.48 | 4.94 | 4.94 |
| 455.47 | 4.34 | 4.34 |