Basic Information
ID: ALA3887255
Type: Binding
Description: SPA Assay: The assays were performed in U-bottom 384-well optiplates. The final assay volume was 15 ul prepared from 7.5 ul additions of microsomes (prepared as a high-speed pellet from homogenized HEK2 cells stably transfected with CYP17), substrates (3H-Pregnenolone and NADPH) and test compounds in assay buffer (50 mM Potassium phosphate pH 7.2, 10% glycerol). The reaction was initiated by the combination of the microsomes and substrates in wells containing compound. The reaction was incubated at room temperature for 45 minutes and terminated by adding 7.5 ul of 0.2N HCl to each well. Following an incubation period of 10 minutes, anti-DHEA-coated SPA beads were added to the terminated reaction. The plate was sealed and incubated overnight with shaking at 4 C. The beads were allowed to settle in the plate for 1 hour and the plate read on a TOPCOUNT (Perkin-Elmer) plate reader.Inhibition data were calculated by comparison to no enzyme control reactions for 100% inhibition.
Format: BAO_0000219
Organism: Homo sapiens
Cell type: HEK2
Target: Cytochrome P450 17A1(ALA3522)
Document: ALA3886242
BindingDB Source: 7192
Activity Charts
Bioactivity
Activity Types for Assay ALA3887255
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 286.34 | 3.79 | 3.79 |
| 287.33 | 3.19 | 3.19 |
| 354.34 | 4.81 | 4.81 |
| 304.33 | 3.93 | 3.93 |
| 322.32 | 4.07 | 4.07 |
| 287.33 | 3.19 | 3.19 |
| 286.34 | 3.79 | 3.79 |
| 291.28 | 3.97 | 3.97 |
| 248.29 | 2.93 | 2.93 |
| 249.32 | 3.74 | 3.74 |
| 311.69 | 4.31 | 4.31 |
| 293.25 | 2.64 | 2.64 |
| 322.29 | 3.45 | 3.45 |
| 323.31 | 3.34 | 3.34 |
| 287.33 | 3.19 | 3.19 |