ID: ALA3887279
Type: Binding
Description: null: Microsomal preparation: One lobe of fresh pig liver is obtained (e.g., at about the time of slaughter from a food-processing company) and immediately placed in ice cold 15 mM KH2PO4/250 mM sucrose (pH 7.4) and kept on ice during transportation. A 10 g sample of liver is minced and homogenized in 30 mL of homogenization buffer (15 mM KH2PO4/250 mM sucrose) using a Tekmar homoginizer or equivalent by pulsing 3 times with 20 second pulses. This procedure is repeated for a total of 8x10 g samples of pig liver. The remaining pig liver may be stored at -80 C. The homogenates from the 8 samples are pooled and centrifuged at 13,000xg for 20 minutes at 4 C. to remove crude debris. The supernatant is further centrifuged at 100,000xg for 70 minutes at 4° C. The microsomal pellets are re-suspended into 50 mL of 150 mM KH2PO4/1 mM DTT (pH 7.4) and 1 mL aliquots are stored at -80 C.100-150 ug of pig liver microsomal protein in 150 mM KH2PO4 is incubated at 37° C.
Organism: Homo sapiens
Bioactivity
Activity Types for Assay ALA3887279
IC50
Parent Molecular Weight | ALogP | Polar Surface Area |
---|---|---|
144.18 | 2.08 | 2.08 |
144.18 | 2.08 | 2.08 |
158.20 | 1.93 | 1.93 |
144.18 | 1.87 | 1.87 |
344.85 | 5.38 | 5.38 |
531.44 | 4.21 | 4.21 |
220.28 | 3.74 | 3.74 |
172.23 | 2.24 | 2.24 |
292.77 | 3.73 | 3.73 |