Basic Information
ID: ALA3887296
Type: Binding
Description: P70S6K Enzyme Assay: P70S6K inhibitor compounds are diluted and plated in 96 well plates. A reaction mixture including the following components is then added to the compound plate to initiate the enzyme reaction; P70S6K (3 nM, T412E mutant, Millipore) is mixed with 24 uM ATP in an assay buffer containing 100 mM Hepes (pH 7.5), 5 mM MgCl2, 1 mM DTT, 0.015% Brij and 1 uM of the substrate peptide FITC-AHA-AKRRRLSSLRA-OH (derived from the S6 ribosomal protein sequence, FITC=fluorescein isothiocyanate, AHA=6-aminohexanoic acid). The reaction is incubated for 90 min at 25.degree. C., before the addition of 10 mM EDTA to stop the reaction. The proportion of substrate and product (phosphorylated) peptide is analysed on a Caliper Life Sciences Lab Chip 3000, using a pressure of -1.4 psi, and upstream and downstream voltages of -3000 and -700 respectively. Product peaks are resolved before substrate peaks on the resulting chromatograms.
Format: BAO_0000357
Organism: Homo sapiens
Target: Ribosomal protein S6 kinase 1(ALA4501)
Document: ALA3886265
BindingDB Source: 7230
Activity Charts
Bioactivity
Activity Types for Assay ALA3887296
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 499.31 | 4.76 | 4.76 |
| 490.51 | 4.66 | 4.66 |
| 535.57 | 4.68 | 4.68 |
| 484.30 | 5.18 | 5.18 |
| 554.60 | 5.09 | 5.09 |
| 452.28 | 5.03 | 5.03 |
| 521.52 | 5.54 | 5.54 |
| 545.63 | 5.37 | 5.37 |
| 539.51 | 5.68 | 5.68 |
| 478.52 | 6.55 | 6.55 |
| 517.58 | 4.90 | 4.90 |
| 511.53 | 5.37 | 5.37 |
| 549.62 | 4.60 | 4.60 |
| 531.95 | 5.72 | 5.72 |
| 543.61 | 5.45 | 5.45 |
| 448.42 | 3.81 | 3.81 |
| 611.69 | 5.92 | 5.92 |
| 512.52 | 4.65 | 4.65 |
| 547.60 | 4.96 | 4.96 |
| 485.28 | 4.75 | 4.75 |
| 526.54 | 5.68 | 5.68 |
| 530.96 | 6.32 | 6.32 |
| 615.63 | 6.51 | 6.51 |
| 521.52 | 5.54 | 5.54 |
| 526.54 | 5.16 | 5.16 |
| 505.56 | 4.59 | 4.59 |
| 532.49 | 5.95 | 5.95 |
| 514.50 | 5.81 | 5.81 |
| 541.56 | 4.52 | 4.52 |
| 502.52 | 3.90 | 3.90 |
| 597.64 | 6.37 | 6.37 |
| 564.51 | 6.69 | 6.69 |
| 499.31 | 4.76 | 4.76 |
| 420.41 | 4.00 | 4.00 |
| 517.56 | 5.43 | 5.43 |
| 570.60 | 4.07 | 4.07 |
| 489.48 | 3.50 | 3.50 |
| 521.52 | 5.54 | 5.54 |
| 434.59 | 3.26 | 3.26 |
| 514.50 | 5.81 | 5.81 |
| 494.52 | 5.53 | 5.53 |
| 467.29 | 4.61 | 4.61 |
| 555.58 | 4.56 | 4.56 |
| 450.44 | 3.49 | 3.49 |
| 498.49 | 4.46 | 4.46 |
| 535.55 | 6.15 | 6.15 |
| 548.63 | 4.48 | 4.48 |
| 503.55 | 4.67 | 4.67 |
| 451.29 | 5.64 | 5.64 |
| 529.59 | 5.20 | 5.20 |
| 611.66 | 6.76 | 6.76 |
| 571.60 | 5.83 | 5.83 |
| 553.56 | 4.98 | 4.98 |
| 514.50 | 5.81 | 5.81 |
| 520.54 | 3.12 | 3.12 |
| 611.64 | 5.48 | 5.48 |
| 517.58 | 4.95 | 4.95 |
| 527.53 | 5.07 | 5.07 |
| 516.57 | 6.04 | 6.04 |
| 480.98 | 3.68 | 3.68 |
| 445.42 | 3.87 | 3.87 |
| 525.55 | 5.13 | 5.13 |
| 597.64 | 6.37 | 6.37 |
| 611.66 | 6.76 | 6.76 |
| 532.55 | 3.27 | 3.27 |
| 513.50 | 4.04 | 4.04 |
| 543.61 | 5.59 | 5.59 |
| 479.51 | 5.95 | 5.95 |
| 500.57 | 3.99 | 3.99 |
| 504.49 | 4.19 | 4.19 |
| 519.55 | 4.18 | 4.18 |
| 517.58 | 5.06 | 5.06 |
| 596.47 | 5.71 | 5.71 |
| 497.50 | 5.06 | 5.06 |
| 556.41 | 4.79 | 4.79 |
| 452.61 | 2.49 | 2.49 |
| 581.62 | 4.47 | 4.47 |
| 498.99 | 2.90 | 2.90 |
| 514.53 | 4.04 | 4.04 |
| 531.60 | 4.98 | 4.98 |
| 463.44 | 3.10 | 3.10 |
| 450.44 | 4.01 | 4.01 |
| 499.49 | 6.23 | 6.23 |
| 494.55 | 3.49 | 3.49 |
| 543.61 | 5.59 | 5.59 |
| 512.57 | 2.71 | 2.71 |
| 530.96 | 6.32 | 6.32 |
| 569.61 | 4.67 | 4.67 |
| 496.51 | 5.67 | 5.67 |
| 547.60 | 5.03 | 5.03 |
| 611.66 | 6.76 | 6.76 |
| 497.50 | 5.06 | 5.06 |
| 515.49 | 5.20 | 5.20 |
| 597.62 | 5.39 | 5.39 |
| 500.48 | 5.80 | 5.80 |
| 515.58 | 4.47 | 4.47 |
| 510.54 | 5.98 | 5.98 |
| 582.44 | 5.32 | 5.32 |
| 526.54 | 5.68 | 5.68 |
| 582.61 | 4.90 | 4.90 |