Basic Information
ID: ALA3887327
Type: Binding
Description: Kinase Glo Plus Assay: Human RSK2 protein, purchased from Invitrogen, is used to measure kinase activity utilizing Kinase Glo Plus (Promega) a homogeneous assay technology, which uses a luciferin-luciferase based ATP detection reagent to quantify residual ATP. The assay is performed using 0.75 nM His-RSK2, 0.75 .mu.M ATP and 1.0 uM S6 Kinase/RSK Substrate Peptide 1 (Upstate, catalog #12-124), in assay buffer consisting of 25 mM HEPES, pH 7.5, 10 mM MgCl2, 5 mM MnCl2, 50 mM KCl, 0.2% BSA, 0.01% CHAPS, 100 uM Na3VO4, 0.5 mM DTT, and 1% DMSO. Solutions of test compounds at various concentrations are prepared by 1:3 fold serial dilution of a 1 mM solution of compound in DMSO. The DMSO solutions are further diluted with assay buffer to a final concentration of DMSO of 5%. The assay is performed in a 384 well, white, non-binding plate (Corning, catalogue #3574). Solutions of test compounds (10 uL) are transferred to a dry assay plate, followed by addition of 20 uL kinase.
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3887327
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 448.53 | 2.78 | 2.78 |
| 324.34 | 2.32 | 2.32 |
| 350.38 | 2.89 | 2.89 |
| 352.39 | 3.14 | 3.14 |
| 386.41 | 3.68 | 3.68 |
| 323.36 | 1.76 | 1.76 |
| 405.50 | 3.80 | 3.80 |
| 400.44 | 2.67 | 2.67 |
| 373.42 | 2.91 | 2.91 |
| 387.44 | 3.40 | 3.40 |
| 401.47 | 3.96 | 3.96 |
| 435.49 | 4.37 | 4.37 |
| 444.54 | 3.33 | 3.33 |
| 421.89 | 4.05 | 4.05 |
| 478.98 | 3.98 | 3.98 |
| 388.43 | 2.79 | 2.79 |
| 458.57 | 3.89 | 3.89 |
| 458.57 | 3.89 | 3.89 |
| 472.59 | 3.96 | 3.96 |
| 334.38 | 2.98 | 2.98 |
| 348.41 | 3.23 | 3.23 |
| 348.41 | 3.06 | 3.06 |
| 356.33 | 2.67 | 2.67 |
| 346.39 | 2.81 | 2.81 |
| 360.42 | 3.20 | 3.20 |
| 319.36 | 3.03 | 3.03 |
| 353.81 | 3.68 | 3.68 |
| 353.81 | 3.68 | 3.68 |
| 353.81 | 3.68 | 3.68 |
| 349.39 | 3.04 | 3.04 |
| 362.39 | 2.13 | 2.13 |
| 320.35 | 2.42 | 2.42 |
| 320.35 | 2.42 | 2.42 |
| 385.43 | 3.42 | 3.42 |
| 309.33 | 1.75 | 1.75 |
| 323.36 | 1.76 | 1.76 |
| 309.33 | 1.75 | 1.75 |
| 323.36 | 1.76 | 1.76 |
| 309.33 | 1.75 | 1.75 |
| 370.41 | 3.58 | 3.58 |
| 402.46 | 3.35 | 3.35 |
| 370.41 | 3.58 | 3.58 |
| 360.37 | 3.17 | 3.17 |
| 359.39 | 2.90 | 2.90 |
| 385.43 | 3.42 | 3.42 |
| 484.56 | 3.56 | 3.56 |
| 413.48 | 3.83 | 3.83 |
| 349.39 | 3.04 | 3.04 |
| 351.41 | 2.80 | 2.80 |
| 413.48 | 3.58 | 3.58 |
| 434.50 | 2.43 | 2.43 |
| 324.34 | 2.49 | 2.49 |
| 338.37 | 2.57 | 2.57 |
| 431.50 | 3.10 | 3.10 |
| 386.41 | 3.68 | 3.68 |
| 513.65 | 3.31 | 3.31 |
| 393.45 | 2.58 | 2.58 |
| 401.47 | 4.13 | 4.13 |
| 351.37 | 1.83 | 1.83 |
| 413.48 | 3.83 | 3.83 |
| 415.50 | 4.52 | 4.52 |
| 320.35 | 2.59 | 2.59 |
| 387.40 | 3.08 | 3.08 |
| 458.57 | 3.67 | 3.67 |
| 436.52 | 2.63 | 2.63 |
| 429.52 | 4.76 | 4.76 |
| 435.43 | 3.52 | 3.52 |
| 344.37 | 2.90 | 2.90 |
| 338.37 | 2.88 | 2.88 |
| 366.42 | 3.48 | 3.48 |
| 385.43 | 3.21 | 3.21 |
| 393.83 | 3.56 | 3.56 |
| 399.45 | 3.44 | 3.44 |
| 387.44 | 3.26 | 3.26 |
| 519.51 | 3.34 | 3.34 |
| 407.86 | 3.57 | 3.57 |
| 401.47 | 4.13 | 4.13 |
| 472.59 | 4.14 | 4.14 |
| 366.42 | 3.31 | 3.31 |
| 352.39 | 2.97 | 2.97 |
| 344.37 | 2.90 | 2.90 |
| 390.47 | 3.95 | 3.95 |
| 458.57 | 3.57 | 3.57 |
| 382.49 | 3.78 | 3.78 |
| 384.40 | 2.78 | 2.78 |
| 350.38 | 2.89 | 2.89 |
| 366.42 | 3.31 | 3.31 |
| 401.47 | 3.55 | 3.55 |
| 400.44 | 3.99 | 3.99 |
| 344.37 | 2.90 | 2.90 |
| 350.38 | 2.43 | 2.43 |
| 351.41 | 2.80 | 2.80 |
| 373.42 | 3.21 | 3.21 |
| 338.37 | 2.88 | 2.88 |
| 457.54 | 2.66 | 2.66 |
| 377.38 | 3.04 | 3.04 |
| 387.44 | 3.47 | 3.47 |
| 399.45 | 3.43 | 3.43 |
| 472.59 | 3.96 | 3.96 |
| 456.55 | 3.64 | 3.64 |
| 407.86 | 3.57 | 3.57 |
| 338.37 | 2.58 | 2.58 |
| 399.45 | 3.27 | 3.27 |
| 425.49 | 3.66 | 3.66 |
| 507.04 | 4.62 | 4.62 |
| 380.45 | 3.87 | 3.87 |
| 444.54 | 3.50 | 3.50 |
| 379.46 | 3.27 | 3.27 |
| 459.55 | 3.78 | 3.78 |
| 350.38 | 2.43 | 2.43 |
| 470.58 | 3.72 | 3.72 |
| 350.38 | 2.71 | 2.71 |
| 360.32 | 2.57 | 2.57 |
| 439.52 | 4.05 | 4.05 |
| 392.46 | 4.06 | 4.06 |
| 472.55 | 2.71 | 2.71 |
| 376.42 | 2.69 | 2.69 |
| 350.38 | 1.73 | 1.73 |
| 348.41 | 3.29 | 3.29 |
| 388.43 | 2.62 | 2.62 |
| 352.39 | 3.14 | 3.14 |
| 358.79 | 2.98 | 2.98 |
| 442.52 | 3.34 | 3.34 |
| 492.97 | 3.39 | 3.39 |
| 514.63 | 3.74 | 3.74 |
| 382.42 | 2.29 | 2.29 |
| 427.39 | 3.92 | 3.92 |
| 337.38 | 2.32 | 2.32 |
| 323.36 | 2.00 | 2.00 |
| 400.44 | 4.03 | 4.03 |
| 430.51 | 2.94 | 2.94 |
| 351.41 | 2.40 | 2.40 |
| 417.49 | 3.47 | 3.47 |
| 444.54 | 3.50 | 3.50 |
| 401.47 | 3.71 | 3.71 |
| 400.44 | 3.99 | 3.99 |
| 413.48 | 3.47 | 3.47 |
| 458.57 | 3.89 | 3.89 |
| 427.51 | 4.49 | 4.49 |
| 385.43 | 3.21 | 3.21 |
| 364.41 | 3.07 | 3.07 |
| 427.51 | 4.08 | 4.08 |
| 394.48 | 2.18 | 2.18 |
| 401.47 | 3.74 | 3.74 |
| 407.86 | 4.12 | 4.12 |
| 383.48 | 3.18 | 3.18 |
| 431.50 | 3.15 | 3.15 |
| 376.44 | 3.64 | 3.64 |
| 351.38 | 3.73 | 3.73 |
| 427.51 | 3.91 | 3.91 |
| 411.46 | 4.82 | 4.82 |
| 385.43 | 3.42 | 3.42 |
| 470.58 | 3.72 | 3.72 |
| 387.44 | 3.16 | 3.16 |
| 416.49 | 3.91 | 3.91 |
| 373.42 | 3.46 | 3.46 |
| 417.44 | 3.41 | 3.41 |
| 390.47 | 3.95 | 3.95 |
| 484.60 | 4.11 | 4.11 |
| 588.61 | 3.32 | 3.32 |
| 464.53 | 4.01 | 4.01 |
| 338.37 | 2.88 | 2.88 |
| 401.47 | 4.03 | 4.03 |
| 338.37 | 2.88 | 2.88 |
| 340.41 | 2.79 | 2.79 |
| 441.41 | 3.94 | 3.94 |
| 401.47 | 3.96 | 3.96 |
| 458.57 | 3.89 | 3.89 |
| 365.44 | 2.98 | 2.98 |
| 363.42 | 2.54 | 2.54 |
| 338.37 | 2.67 | 2.67 |
| 352.39 | 2.96 | 2.96 |
| 359.39 | 3.07 | 3.07 |
| 413.48 | 3.83 | 3.83 |
| 385.43 | 3.42 | 3.42 |
| 401.47 | 3.96 | 3.96 |
| 378.31 | 3.03 | 3.03 |
| 415.50 | 4.13 | 4.13 |
| 321.34 | 1.82 | 1.82 |
| 413.48 | 3.62 | 3.62 |
| 458.57 | 3.89 | 3.89 |
| 361.45 | 4.15 | 4.15 |
| 422.49 | 1.56 | 1.56 |
| 415.52 | 5.06 | 5.06 |
| 429.52 | 4.69 | 4.69 |
| 380.45 | 1.79 | 1.79 |
| 366.42 | 3.31 | 3.31 |
| 448.53 | 2.99 | 2.99 |
| 404.47 | 2.86 | 2.86 |
| 348.41 | 3.23 | 3.23 |
| 337.38 | 2.39 | 2.39 |
| 453.57 | 3.92 | 3.92 |
| 334.38 | 2.98 | 2.98 |
| 373.42 | 3.08 | 3.08 |
| 379.42 | 2.19 | 2.19 |
| 324.34 | 2.49 | 2.49 |
| 387.44 | 3.57 | 3.57 |
| 364.41 | 3.10 | 3.10 |
| 400.44 | 3.61 | 3.61 |
| 393.45 | 2.30 | 2.30 |
| 469.55 | 3.68 | 3.68 |
| 358.40 | 3.68 | 3.68 |
| 374.40 | 2.31 | 2.31 |
| 480.52 | 3.57 | 3.57 |
| 349.39 | 3.04 | 3.04 |
| 468.56 | 3.25 | 3.25 |
| 376.42 | 2.47 | 2.47 |
| 367.41 | 1.87 | 1.87 |
| 413.48 | 3.52 | 3.52 |
| 433.51 | 2.32 | 2.32 |
| 361.45 | 4.15 | 4.15 |
| 427.51 | 3.89 | 3.89 |
| 323.36 | 1.93 | 1.93 |
| 404.40 | 3.82 | 3.82 |
| 376.42 | 2.39 | 2.39 |
| 361.40 | 3.23 | 3.23 |
| 373.42 | 3.25 | 3.25 |
| 324.34 | 2.32 | 2.32 |
| 323.36 | 2.06 | 2.06 |
| 415.50 | 4.52 | 4.52 |
| 352.39 | 3.13 | 3.13 |
| 401.47 | 3.96 | 3.96 |
| 337.38 | 2.32 | 2.32 |
| 399.45 | 3.44 | 3.44 |
| 401.47 | 4.13 | 4.13 |
| 416.49 | 3.91 | 3.91 |
| 465.54 | 3.09 | 3.09 |
| 338.37 | 2.88 | 2.88 |
| 338.37 | 2.88 | 2.88 |
| 334.38 | 2.98 | 2.98 |
| 427.51 | 4.08 | 4.08 |
| 413.48 | 3.83 | 3.83 |
| 458.57 | 3.89 | 3.89 |
| 415.50 | 4.52 | 4.52 |
| 324.34 | 2.49 | 2.49 |
| 444.54 | 3.50 | 3.50 |
| 413.48 | 3.83 | 3.83 |