Basic Information
ID: ALA3887383
Type: Binding
Description: Inhibition Assay: 6 data point. Cdc7 kinase assays were carried out in 25 mM HEPES, pH 7.5, 1 mM DTT, 10 mM MgCl2, 100 μM Na3VO4, and 0.075 mg/ml Triton X-100 using 12 ng baculovirus-expressed Cdc7/Dbf4 and 2 μM Jerini peptide substrate A-A11 (biotin-C6linker-TPSDSLIYDDGLS) (SEQ ID NO:5). Reactions were initiated with λ-[33P]-ATP (1 μM, 20 mCi/μmol) and quenched after 1 hour with 5 volumes of stop buffer (50 mM EDTA, 2 M NaCl). The reactions were incubated for 30 minutes on streptavidin-coated plates, washed, and quantified using a TopCount scintillation plate reader (Packard). IC50 values are determined via non-linear regression fitting of the data. Ki values are generated assuming ATP-competitive (equilibrium) inhibition and using the experimentally determined apparent ATP Km of Cdc7 (0.7 μM).
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3887383
Ki
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 411.31 | 4.91 | 4.91 |
| 375.44 | 2.37 | 2.37 |
| 374.45 | 3.35 | 3.35 |
| 283.34 | 2.25 | 2.25 |
| 366.43 | 4.12 | 4.12 |
| 416.23 | 3.06 | 3.06 |
| 360.42 | 3.61 | 3.61 |
| 415.50 | 2.91 | 2.91 |
| 384.42 | 4.26 | 4.26 |
| 390.45 | 2.59 | 2.59 |
| 304.36 | 2.76 | 2.76 |
| 346.39 | 2.57 | 2.57 |
| 394.44 | 4.12 | 4.12 |
| 412.43 | 4.26 | 4.26 |
| 436.52 | 5.28 | 5.28 |
| 431.50 | 1.93 | 1.93 |
| 401.47 | 2.43 | 2.43 |
| 470.54 | 5.79 | 5.79 |
| 388.43 | 2.34 | 2.34 |
| 429.48 | 2.17 | 2.17 |
| 421.89 | 4.13 | 4.13 |
| 386.46 | 3.50 | 3.50 |
| 452.49 | 5.14 | 5.14 |
| 394.44 | 4.12 | 4.12 |
| 420.52 | 5.36 | 5.36 |
| 388.48 | 3.81 | 3.81 |