GTPase Activity Assay: GTPase assays were performed in 50 mM MES, pH 6.5, 50 mM KCl, 5 mM MgCl2 in the presence of 2 uM FtsZ in the presence of varyin...

ID: ALA3887390

Type: Binding

Description: GTPase Activity Assay: GTPase assays were performed in 50 mM MES, pH 6.5, 50 mM KCl, 5 mM MgCl2 in the presence of 2 uM FtsZ in the presence of varying concentrations of compound 1. Solutions were incubated at room temperature for 5 min, followed by addition of 0.25 mM GTP. At the end of a 20-min incubation period, enzyme activity was quenched by addition of 50 uL Malachite Green solution and the wells read at A625 on an absorbance plate reader.

Format: BAO_0000357

Target:  Cell division protein ftsZ(ALA5096)

Document:  ALA3886301

BindingDB Source:  7281