Basic Information
ID: ALA3887420
Type: Binding
Description: LC/MS assay: To screen for SPR inhibition, a biochemical assay based on LC/MS (and chromogenic) read-out has been developed. The LC/MS assay monitors the product formation (L-biopterin) and the chromogenic assay measures OD at 420 nm.N-methoxyacetyl serotonin was used as a reference compound (positive control). The IC50 measured using the screening conditions was 20-40 nM, which agrees with the literature (Smith et al., Journal of Biological Chemistry, 297:5601, 1992).The exemplary assay protocol uses the following conditions: SPR (6 nM); L-Sepiapterin (50 uM); NADPH (100 uM); Na-Phosphate buffer, pH 6.5 (100 mM); 82 uL assay volume; 60 minutes incubation with compounds (0.5% final concentration in DMSO) at 37° C. in Greiner uClear 384 well plates.The following experimental procedure was applied: (1) Add 2 uL compound (inhibitor) dilutions (20% DMSO) in Greiner uClear 384 well plates. (2) Add 40 uL enzyme/assay buffer.
Format: BAO_0000357
Target: Sepiapterin reductase(ALA3988583)
Document: ALA3886312
BindingDB Source: 7297
Activity Charts
Bioactivity
Activity Types for Assay ALA3887420
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 209.25 | 0.70 | 0.70 |
| 325.39 | 0.84 | 0.84 |
| 246.31 | 2.19 | 2.19 |
| 262.31 | 1.57 | 1.57 |
| 244.29 | 1.94 | 1.94 |
| 262.31 | 1.49 | 1.49 |
| 250.27 | 1.61 | 1.61 |
| 253.31 | 2.92 | 2.92 |
| 310.38 | 0.88 | 0.88 |
| 262.31 | 1.19 | 1.19 |
| 262.27 | 0.82 | 0.82 |
| 276.34 | 1.50 | 1.50 |