Basic Information
ID: ALA3887421
Type: Binding
Description: Enzymatic Assay: The enzymatic assay was measured using white, opaque 96-well half area plates. Each well contained 60 μL of assay buffer (50 mM HEPES pH 8.0, 50 mM NaCl and 1 mM CaCl2) and 2 ul of a DMSO solution containing compound of interest. Conditioned media obtained from HT-1080 cells, which were transformed by RAGE technology (Athersys) to overexpress endogenous EL, was added and the reaction was allowed to incubate for 20 min at 37° C. with gentle agitation. The reaction was started by the addition of 20 μL of a 1:4 dilution of vesicles. The final total reaction volume was 100 μL. The reaction rates were measured on a Gemini plate reader with an excitation wavelength of 488 nm and an emission of 530 nm. Readings were taken every 20 seconds for 10 min with agitation between each reading.
Organism: Homo sapiens
Cell type: HT-1080
Activity Charts
Bioactivity
Activity Types for Assay ALA3887421
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 401.47 | 2.23 | 2.23 |
| 379.40 | 2.47 | 2.47 |
| 429.48 | 2.21 | 2.21 |
| 460.60 | 5.80 | 5.80 |
| 448.46 | 1.98 | 1.98 |
| 555.55 | 5.05 | 5.05 |
| 429.48 | 2.43 | 2.43 |
| 450.54 | 5.16 | 5.16 |
| 450.48 | 1.43 | 1.43 |
| 474.52 | 5.38 | 5.38 |
| 498.54 | 4.49 | 4.49 |
| 536.63 | 6.30 | 6.30 |
| 447.43 | 3.04 | 3.04 |
| 478.53 | 6.02 | 6.02 |
| 495.52 | 4.24 | 4.24 |
| 476.54 | 4.69 | 4.69 |
| 478.94 | 6.02 | 6.02 |
| 450.52 | 5.16 | 5.16 |
| 488.51 | 2.05 | 2.05 |
| 429.48 | 1.91 | 1.91 |
| 379.40 | 2.75 | 2.75 |
| 433.45 | 2.72 | 2.72 |
| 534.56 | 6.98 | 6.98 |
| 419.47 | 3.22 | 3.22 |
| 380.40 | 4.29 | 4.29 |
| 429.48 | 2.28 | 2.28 |
| 503.50 | 2.15 | 2.15 |
| 419.47 | 2.90 | 2.90 |
| 490.59 | 5.69 | 5.69 |
| 406.42 | 3.30 | 3.30 |
| 462.53 | 4.96 | 4.96 |
| 401.45 | 4.09 | 4.09 |
| 436.47 | 3.47 | 3.47 |
| 434.48 | 2.33 | 2.33 |
| 498.58 | 6.10 | 6.10 |
| 450.54 | 5.16 | 5.16 |
| 462.48 | 5.51 | 5.51 |
| 504.61 | 6.13 | 6.13 |
| 484.52 | 1.53 | 1.53 |
| 564.59 | 6.86 | 6.86 |
| 427.49 | 4.63 | 4.63 |
| 405.44 | 2.88 | 2.88 |
| 535.55 | 5.92 | 5.92 |
| 421.48 | 3.51 | 3.51 |
| 515.47 | 3.99 | 3.99 |
| 380.43 | 3.64 | 3.64 |
| 404.45 | 3.26 | 3.26 |
| 472.53 | 5.21 | 5.21 |
| 472.61 | 5.63 | 5.63 |
| 484.34 | 3.58 | 3.58 |
| 419.47 | 3.27 | 3.27 |
| 322.35 | 3.16 | 3.16 |
| 400.44 | 2.37 | 2.37 |
| 404.45 | 3.40 | 3.40 |
| 444.49 | 5.37 | 5.37 |
| 451.44 | 2.86 | 2.86 |
| 418.57 | 5.38 | 5.38 |
| 427.49 | 4.63 | 4.63 |
| 436.49 | 4.33 | 4.33 |
| 471.45 | 3.48 | 3.48 |
| 426.48 | 2.94 | 2.94 |
| 508.58 | 5.83 | 5.83 |
| 468.54 | 4.75 | 4.75 |
| 462.48 | 5.51 | 5.51 |
| 537.56 | 4.92 | 4.92 |
| 423.43 | 3.02 | 3.02 |
| 423.43 | 3.02 | 3.02 |
| 452.54 | 5.57 | 5.57 |
| 487.46 | 3.83 | 3.83 |
| 419.42 | 2.33 | 2.33 |
| 475.53 | 3.84 | 3.84 |
| 487.46 | 4.15 | 4.15 |
| 482.57 | 5.37 | 5.37 |
| 433.45 | 2.01 | 2.01 |
| 418.43 | 2.50 | 2.50 |
| 469.50 | 5.38 | 5.38 |
| 478.94 | 6.54 | 6.54 |
| 405.44 | 2.88 | 2.88 |
| 475.45 | 4.19 | 4.19 |
| 424.44 | 2.82 | 2.82 |
| 450.52 | 5.16 | 5.16 |
| 452.54 | 5.57 | 5.57 |
| 429.48 | 2.43 | 2.43 |