Basic Information
ID: ALA3887437
Type: Binding
Description: In Vitro Enzyme Assay: In vitro enzyme assays were conducted via the Ba(OH)2 precipitation method of Wang et al. (32) using recombinant human PDE11A4, PDE10A1, PDE5A1, PDE6C, PDE8A (BPS Bioscience Inc.), PDE7A (BIOMOL International), and PDE4A10 enzymes (gift from HengmingKe), in the presence of 100 nM cGMP, 30 nM cAMP, 500 nM cGMP, 1.7 μM cGMP, 10 nM cAMP, 15 nM cAMP, and 625 nM cAMP, respectively. Inhibitor concentrations that reduce enzyme activity by 50% (IC50) are presented. The values are means of at least three independent experiments.
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3887437
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 394.45 | 6.28 | 6.28 |
| 334.47 | 3.39 | 3.39 |
| 318.47 | 3.77 | 3.77 |
| 304.44 | 3.38 | 3.38 |
| 318.47 | 3.69 | 3.69 |
| 318.47 | 3.77 | 3.77 |
| 404.54 | 5.25 | 5.25 |
| 295.32 | 2.06 | 2.06 |