Basic Information
ID: ALA3887451
Type: Binding
Description: In Vitro Assay: FVIIa-Xase Ki (37° C.): S2765 (0.5 mM), PCPS (25 μM), calcium chloride (5 mM), full-length human TF (3 nM), human FVIIa (5 pM), and FVIIa inhibitor were incubated for 15 min at 37° C. Reactions were initiated by the addition of human FX (range of concentrations). Preliminary experiments revealed that the plasma purified FX contains a residual amount of human FVIIa which could not be removed by affinity chromatography. The residual FVIIa is sufficient, when combined with PCPS, calcium and TF to catalyze the conversion of FX to Xa, and was increased by the addition of 5 pM human FVIIa. FXa in turn hydrolyses S2765, which was monitored for 60 min at 405 nm.
Organism: Homo sapiens
Tissue: Plasma
Activity Charts
Bioactivity
Activity Types for Assay ALA3887451
Ki
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 481.56 | 4.95 | 4.95 |
| 613.74 | 5.54 | 5.54 |
| 629.69 | 4.88 | 4.88 |
| 663.77 | 5.55 | 5.55 |
| 677.83 | 6.72 | 6.72 |
| 611.67 | 5.86 | 5.86 |
| 665.72 | 5.52 | 5.52 |
| 631.73 | 5.68 | 5.68 |
| 631.73 | 5.68 | 5.68 |
| 617.71 | 5.20 | 5.20 |
| 654.74 | 5.74 | 5.74 |
| 621.66 | 6.43 | 6.43 |
| 633.75 | 5.92 | 5.92 |
| 651.74 | 6.06 | 6.06 |
| 631.73 | 5.68 | 5.68 |
| 622.70 | 6.57 | 6.57 |
| 601.68 | 5.50 | 5.50 |
| 608.74 | 6.08 | 6.08 |
| 649.72 | 5.82 | 5.82 |
| 615.71 | 6.16 | 6.16 |
| 649.72 | 5.78 | 5.78 |
| 580.69 | 5.30 | 5.30 |
| 613.69 | 5.91 | 5.91 |
| 619.72 | 5.54 | 5.54 |
| 629.78 | 6.18 | 6.18 |
| 592.70 | 5.45 | 5.45 |
| 613.69 | 6.32 | 6.32 |
| 649.72 | 5.82 | 5.82 |
| 651.74 | 6.06 | 6.06 |
| 615.71 | 6.16 | 6.16 |
| 627.72 | 5.89 | 5.89 |
| 631.73 | 5.49 | 5.49 |
| 601.68 | 5.50 | 5.50 |
| 649.67 | 6.57 | 6.57 |
| 645.70 | 6.49 | 6.49 |
| 601.68 | 5.50 | 5.50 |
| 613.69 | 5.91 | 5.91 |
| 649.72 | 5.84 | 5.84 |
| 559.65 | 4.18 | 4.18 |
| 645.76 | 4.57 | 4.57 |
| 605.69 | 5.05 | 5.05 |
| 713.81 | 7.02 | 7.02 |
| 587.70 | 4.80 | 4.80 |