Basic Information
ID: ALA3887452
Type: Binding
Description: In Vitro Assay: Tissue kallikrein-1 (37° C) activity was determined in reactions containing 0.05 nM enzyme and 90 μM substrate (H-D-Val-Leu-Arg-AFC) in buffer (0.1M sodium phosphate pH 7.4, 0.2 M NaCl, 0.5% PEG 8000, and 1% DMSO). Assays were performed using 96-well microtiter plates (COSTAR 3600, CORNING, NY, USA) and a thermostatic temperature controlled plate reader (SPECTRAMAX® Gemini, Molecular Devices, Sunnyvale, Calif., USA). Fluorescence was monitored using 400 nm excitation and 505 nm emission wavelengths.
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3887452
Ki
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 613.74 | 5.54 | 5.54 |
| 629.69 | 4.88 | 4.88 |
| 663.77 | 5.55 | 5.55 |
| 677.83 | 6.72 | 6.72 |
| 611.67 | 5.86 | 5.86 |
| 665.72 | 5.52 | 5.52 |
| 631.73 | 5.68 | 5.68 |
| 631.73 | 5.68 | 5.68 |
| 617.71 | 5.20 | 5.20 |
| 654.74 | 5.74 | 5.74 |
| 621.66 | 6.43 | 6.43 |
| 633.75 | 5.92 | 5.92 |
| 651.74 | 6.06 | 6.06 |
| 631.73 | 5.68 | 5.68 |
| 622.70 | 6.57 | 6.57 |
| 601.68 | 5.50 | 5.50 |
| 608.74 | 6.08 | 6.08 |
| 649.72 | 5.82 | 5.82 |
| 615.71 | 6.16 | 6.16 |
| 649.72 | 5.78 | 5.78 |
| 580.69 | 5.30 | 5.30 |
| 613.69 | 5.91 | 5.91 |
| 619.72 | 5.54 | 5.54 |
| 629.78 | 6.18 | 6.18 |
| 592.70 | 5.45 | 5.45 |
| 613.69 | 6.32 | 6.32 |
| 649.72 | 5.82 | 5.82 |
| 651.74 | 6.06 | 6.06 |
| 615.71 | 6.16 | 6.16 |
| 627.72 | 5.89 | 5.89 |
| 631.73 | 5.49 | 5.49 |
| 601.68 | 5.50 | 5.50 |
| 649.67 | 6.57 | 6.57 |
| 645.70 | 6.49 | 6.49 |
| 601.68 | 5.50 | 5.50 |
| 613.69 | 5.91 | 5.91 |
| 649.72 | 5.84 | 5.84 |
| 559.65 | 4.18 | 4.18 |
| 645.76 | 4.57 | 4.57 |
| 605.69 | 5.05 | 5.05 |
| 713.81 | 7.02 | 7.02 |
| 587.70 | 4.80 | 4.80 |