Basic Information
ID: ALA3887505
Type: Binding
Description: Inhibition Assay: The cells expressing mouse EP1 receptor were seeded at 104 cells/well in 96-well plates and cultured for 2 days with 10% Fetal Bovine Serum (FBS)/alpha Modified Eagle Medium (alphaMEM) in the incubator (37° C., 5% CO2). The cells were washed with phosphate buffer, and load buffer (10% FBS/αMEM containing Fura2/AM (5 uM), indomethacin (20 uM) and probenecid (2.5 mM)) was then added to each well and cells were left standing for 1 hour. Load buffer of each well was discarded and assay buffer (Hank's Balanced Salt Solution (HBSS) containing indomethacin (2.5 mM), probenecid (2.5 mM), HEPES-NaOH (10 mM) and 0.1% (w/v) Bovine Serum Albumin (BSA)) was added to each well and plates were left at room temperature in a dark room for 1 hour. Afterwards, compounds of the present invention (10 uL) or PGE2 (10 uL) prepared with assay buffer was added to each well and intracellular calcium concentrations were measured using a Fluorescence Drug Screening System.
Organism: Mus musculus
Cell type: 104
Activity Charts
Bioactivity
Activity Types for Assay ALA3887505
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 471.58 | 5.33 | 5.33 |
| 500.64 | 5.07 | 5.07 |
| 509.62 | 5.60 | 5.60 |
| 508.64 | 5.53 | 5.53 |
| 513.02 | 4.71 | 4.71 |
| 500.64 | 5.07 | 5.07 |
| 497.61 | 5.51 | 5.51 |
| 482.60 | 5.14 | 5.14 |
| 485.60 | 5.64 | 5.64 |
| 533.08 | 5.08 | 5.08 |
| 514.63 | 4.59 | 4.59 |
| 554.61 | 6.00 | 6.00 |