ID: ALA3887511
Type: Binding
Description: Spectrophotometric Assay: This novel assay was used to determine the kinetic parameters for most of the QC substrates. QC activity was analyzed spectrophotometrically using a continuous method, that was derived by adapting a previous discontinuous assay (Bateman, R. C. J. 1989 J Neurosci Methods 30, 23-28) utilizing glutamate dehydrogenase as auxiliary enzyme. Samples consisted of the respective QC substrate, 0.3 mM NADH, 14 mM α-Ketoglutaric acid and 30 U/ml glutamate dehydrogenase in a final volume of 250 ul. Reactions were started by addition of QC and perused by monitoring of the decrease in absorbance at 340 nm for 8-15 min.The initial velocities were evaluated and the enzymatic activity was determined from a standard curve of ammonia under assay conditions. All samples were measured at 30° C., using either the SPECTRAFluor Plus or the Sunrise (both from TECAN) reader for microplates. Kinetic data was evaluated using GraFit software.
Organism: Homo sapiens
Bioactivity
Activity Types for Assay ALA3887511
Ki
Parent Molecular Weight | ALogP | Polar Surface Area |
---|---|---|
306.39 | 3.87 | 3.87 |
366.45 | 3.88 | 3.88 |
366.45 | 3.88 | 3.88 |
350.40 | 3.60 | 3.60 |
342.37 | 4.14 | 4.14 |
384.49 | 4.39 | 4.39 |
416.48 | 3.07 | 3.07 |
289.34 | 3.06 | 3.06 |
319.37 | 3.07 | 3.07 |
319.37 | 3.07 | 3.07 |
349.39 | 3.08 | 3.08 |
339.79 | 3.53 | 3.53 |
367.41 | 4.66 | 4.66 |
305.34 | 2.88 | 2.88 |
336.42 | 3.88 | 3.88 |
342.37 | 4.14 | 4.14 |
396.47 | 3.89 | 3.89 |
324.43 | 4.37 | 4.37 |
366.45 | 3.88 | 3.88 |
352.49 | 4.59 | 4.59 |
324.38 | 4.01 | 4.01 |
309.76 | 3.52 | 3.52 |
336.42 | 3.88 | 3.88 |
354.80 | 4.06 | 4.06 |
370.87 | 4.53 | 4.53 |
307.38 | 3.57 | 3.57 |
344.21 | 4.18 | 4.18 |
370.87 | 4.53 | 4.53 |