Basic Information
ID: ALA3887514
Type: Functional
Description: GPR6 In Vitro Assay (A.1): This cell based assay measures the ability of compounds to inhibit the constitutive cAMP activity of GPR6 receptor expressed in CHO-K1 cells. CHO cells were stably expressed with GPR6 receptor, whose expression is controlled by a tetracycline inducable element. The cells were cultured in medium containing F12K, 10% FBS, 1% PennStrep, 200 ug/mL Hygromycin. GPR6 receptor expression was induced for 20 hrs with 1 ug/ml doxycycline (sigma D9891) in growth media. After addition of doxycycline cells were plated at a density of 250-500 cells per well in half-volume black clear bottom plates (Costar) and place in an incubator (37° C, 5% C(O)2) for 20 hours prior to cAMP assays.Culture media was removed from cells and they were washed with 50 uL of Ringer's Buffer (MgCl2 0.047 mg/mL, NaH2PO4 0.18 mg/mL, Na2HPO4 0.1 mg/mL, KCl 0.34 mg/mL, NaHC(O)3 1.26 mg/mL, D-glucose 1.8 mg/mL, NaCl 7 mg/mL; pH=7.4). Compounds suspended in DMSO were diluted in Ringer's Buffer.
Format: BAO_0000219
Cell type: CHO
Target: G-protein coupled receptor 6(ALA3714130)
Document: ALA3886348
BindingDB Source: 7355
Activity Charts
Bioactivity
Activity Types for Assay ALA3887514
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 500.59 | 4.20 | 4.20 |
| 517.55 | 3.19 | 3.19 |
| 498.53 | 3.81 | 3.81 |
| 452.53 | 3.26 | 3.26 |
| 517.47 | 4.02 | 4.02 |
| 469.56 | 3.54 | 3.54 |
| 501.58 | 3.69 | 3.69 |
| 517.55 | 3.36 | 3.36 |
| 474.56 | 3.66 | 3.66 |
| 507.53 | 4.34 | 4.34 |
| 429.54 | 3.48 | 3.48 |
| 502.61 | 3.27 | 3.27 |
| 511.52 | 3.40 | 3.40 |
| 498.60 | 3.68 | 3.68 |
| 473.98 | 4.09 | 4.09 |
| 490.57 | 4.54 | 4.54 |
| 511.52 | 3.40 | 3.40 |
| 503.45 | 3.63 | 3.63 |
| 496.51 | 3.52 | 3.52 |
| 490.57 | 4.54 | 4.54 |
| 503.02 | 4.23 | 4.23 |
| 489.57 | 3.72 | 3.72 |
| 511.52 | 3.40 | 3.40 |
| 467.47 | 3.38 | 3.38 |
| 486.59 | 3.53 | 3.53 |
| 431.51 | 4.24 | 4.24 |
| 445.51 | 3.53 | 3.53 |
| 470.52 | 3.42 | 3.42 |
| 481.49 | 3.95 | 3.95 |
| 459.54 | 4.09 | 4.09 |
| 485.58 | 4.48 | 4.48 |
| 489.57 | 3.54 | 3.54 |
| 469.56 | 3.54 | 3.54 |
| 427.52 | 3.23 | 3.23 |
| 496.51 | 3.52 | 3.52 |
| 481.49 | 3.77 | 3.77 |
| 504.58 | 3.34 | 3.34 |
| 461.53 | 4.41 | 4.41 |
| 509.62 | 3.72 | 3.72 |
| 511.52 | 3.57 | 3.57 |
| 503.02 | 4.23 | 4.23 |
| 525.55 | 3.79 | 3.79 |
| 478.57 | 3.65 | 3.65 |
| 495.60 | 3.50 | 3.50 |
| 498.60 | 3.68 | 3.68 |
| 473.98 | 4.09 | 4.09 |
| 445.97 | 4.18 | 4.18 |
| 504.58 | 3.34 | 3.34 |
| 481.49 | 3.95 | 3.95 |
| 461.53 | 4.41 | 4.41 |
| 470.52 | 3.42 | 3.42 |
| 507.53 | 4.34 | 4.34 |
| 507.53 | 4.16 | 4.16 |
| 475.54 | 3.15 | 3.15 |
| 431.51 | 4.24 | 4.24 |
| 488.58 | 4.22 | 4.22 |
| 510.54 | 3.91 | 3.91 |
| 486.59 | 3.53 | 3.53 |
| 511.52 | 3.57 | 3.57 |
| 517.55 | 3.36 | 3.36 |
| 445.97 | 4.18 | 4.18 |
| 453.48 | 3.86 | 3.86 |
| 452.53 | 3.26 | 3.26 |
| 501.58 | 3.69 | 3.69 |
| 503.52 | 2.80 | 2.80 |
| 431.51 | 4.24 | 4.24 |
| 511.52 | 3.40 | 3.40 |
| 525.55 | 3.79 | 3.79 |
| 511.52 | 3.40 | 3.40 |