Basic Information
ID: ALA3887550
Type: Binding
Description: High-Throughput Screening Assay: To identify novel inhibitors of hepsin, the Chembridge DIVERSet 10,000 compound high-diversity library was screened using an assay based on the cleavage of the chromogenic peptide. In addition, the NINDS II library of 1040 compounds (MicroSource Discovery) was screened to identify hepsin inhibitors among established drugs and known bioactive molecules. Screens were performed in a 96-well format at a final compound concentration of 2 uM. To minimize false positives, positions 1 and 12 of each row contained DMSO/buffer controls. As a measure of reproducibility, the Z' score for this assay was 0.78 (Zhang et al., 1999 J Biomol Screen 4:67).
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3887550
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 516.86 | 9.91 | 9.91 |
| 147.13 | -0.87 | -0.87 |
| 226.23 | 2.23 | 2.23 |
| 390.96 | 5.56 | 5.56 |
| 260.34 | 4.68 | 4.68 |
| 218.17 | 1.02 | 1.02 |
| 448.34 | 5.15 | 5.15 |
| 326.78 | 5.07 | 5.07 |
| 494.45 | 5.89 | 5.89 |
| 439.36 | 5.50 | 5.50 |
| 410.23 | 3.60 | 3.60 |
| 444.51 | 3.92 | 3.92 |
| 441.40 | 5.62 | 5.62 |
| 388.88 | 5.03 | 5.03 |
| 360.33 | 5.44 | 5.44 |
| 444.14 | 5.92 | 5.92 |