Basic Information
ID: ALA3887584
Type: Binding
Description: Cellular Enzyme Assay: G-402 cells expressing CYP11 constructs were established as described above and maintained in McCoy's 5a Medium Modified, ATCC Catalog No. 30-2007 containing 10% FCS and 400 ug/ml G418 (Geneticin) at 37° C. under an atmosphere of 5% CO2/95% air. Cellular enzyme assays were performed in DMEM/F12 medium containing 2.5% charcoal treated FCS and appropriate concentration of substrate (0.3-10 uM 11-Deoxycorticosterone, 11-Deoxycortisol or Corticosterone). For assaying enzymatic activity, cells were plated onto 96 well plates and incubated for 16 h. An aliquot of the supernatant is then transferred and analyzed for the concentration of the expected product (Aldosterone for CYP11B2; Cortisol for CYP11B1). The concentrations of these steroids can be determined using HTRF assays from CisBio analyzing either Aldosterone or Cortisol.
Organism: Homo sapiens
Cell type: G-402
Activity Charts
Bioactivity
Activity Types for Assay ALA3887584
EC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 249.32 | 2.96 | 2.96 |
| 291.35 | 3.13 | 3.13 |
| 305.38 | 3.52 | 3.52 |
| 319.41 | 3.77 | 3.77 |
| 316.35 | 3.93 | 3.93 |
| 334.34 | 4.07 | 4.07 |
| 332.81 | 4.44 | 4.44 |
| 332.81 | 4.44 | 4.44 |
| 366.36 | 4.81 | 4.81 |
| 279.26 | 3.75 | 3.75 |
| 296.27 | 3.85 | 3.85 |
| 312.39 | 4.10 | 4.10 |
| 334.34 | 4.28 | 4.28 |
| 400.40 | 3.98 | 3.98 |
| 370.40 | 3.69 | 3.69 |
| 341.44 | 2.94 | 2.94 |
| 336.31 | 3.26 | 3.26 |
| 297.25 | 3.89 | 3.89 |
| 384.42 | 4.08 | 4.08 |
| 362.39 | 4.92 | 4.92 |
| 370.48 | 2.83 | 2.83 |
| 264.33 | 3.64 | 3.64 |
| 318.78 | 4.05 | 4.05 |
| 293.29 | 4.14 | 4.14 |
| 297.25 | 3.89 | 3.89 |
| 337.30 | 3.86 | 3.86 |
| 250.30 | 2.99 | 2.99 |
| 298.36 | 3.71 | 3.71 |
| 314.82 | 4.31 | 4.31 |
| 354.45 | 4.81 | 4.81 |
| 325.30 | 4.52 | 4.52 |
| 291.35 | 3.13 | 3.13 |
| 348.37 | 4.67 | 4.67 |
| 382.41 | 3.84 | 3.84 |
| 264.33 | 3.24 | 3.24 |
| 349.26 | 4.96 | 4.96 |
| 293.29 | 3.92 | 3.92 |
| 318.78 | 4.05 | 4.05 |
| 393.40 | 5.11 | 5.11 |
| 316.35 | 3.93 | 3.93 |
| 364.37 | 3.86 | 3.86 |
| 353.31 | 4.85 | 4.85 |
| 366.36 | 4.81 | 4.81 |
| 362.39 | 4.92 | 4.92 |
| 332.81 | 4.44 | 4.44 |
| 320.40 | 3.32 | 3.32 |
| 356.37 | 3.30 | 3.30 |
| 388.39 | 3.83 | 3.83 |
| 236.27 | 2.60 | 2.60 |
| 321.38 | 3.74 | 3.74 |
| 352.33 | 4.42 | 4.42 |
| 307.31 | 4.38 | 4.38 |
| 278.28 | 3.71 | 3.71 |
| 363.38 | 4.81 | 4.81 |
| 321.38 | 3.74 | 3.74 |
| 352.33 | 4.42 | 4.42 |
| 351.32 | 3.94 | 3.94 |
| 350.34 | 3.52 | 3.52 |
| 352.33 | 4.42 | 4.42 |
| 291.35 | 3.13 | 3.13 |
| 310.29 | 4.24 | 4.24 |
| 364.37 | 3.64 | 3.64 |
| 350.34 | 3.25 | 3.25 |
| 334.34 | 4.28 | 4.28 |
| 362.39 | 4.92 | 4.92 |
| 362.39 | 4.92 | 4.92 |
| 352.33 | 4.42 | 4.42 |
| 318.78 | 4.05 | 4.05 |
| 388.43 | 5.55 | 5.55 |
| 388.39 | 3.83 | 3.83 |