Basic Information
ID: ALA3887621
Type: Binding
Description: Inhibition Assay: The compound was dissolved in dimethyl sulfoxide (DMSO), and diluted to an arbitrary concentration (A). A was 100-fold diluted with a buffer (0.1 M Tris (pH 8.0), 0.15 M NaCl, 10 mM CaCl2, 0.05% Brij38) (B). The r-h trypsin was diluted with a buffer to 0.088 ug/mL, and the m-trypsin was diluted with a buffer to 1/50 (C). The dilution ratio of the m-trypsin (1/50) was set to exhibit the same activity as the 0.088 ug/mL r-h trypsin as determined by kinetic analysis. The substrate solution of a substrate for the enzyme reaction, BZiPAR, (Rhodamine Reference Substrate) was diluted with a buffer to 5 umol/L (D). B; 5 uL, C; 5 uL, and D; 10 uL were added to a 384-plate, and incubated at room temperature for 30 minutes. The fluorescent signals were detected with Ex/Em = 497/520 using Tecan Safire Fluorometer. The compound was reviewed from 2500 nM to its 3-fold value, 0.0075 nM, at 12 concentrations, and the inhibitory rate of each compound was calculated.
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3887621
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 398.42 | 1.39 | 1.39 |
| 596.98 | 3.31 | 3.31 |
| 555.55 | 3.11 | 3.11 |
| 508.96 | 3.56 | 3.56 |
| 529.38 | 3.87 | 3.87 |
| 508.96 | 3.91 | 3.91 |
| 567.00 | 3.41 | 3.41 |
| 559.99 | 3.77 | 3.77 |
| 552.97 | 3.78 | 3.78 |
| 552.97 | 3.54 | 3.54 |
| 567.00 | 4.00 | 4.00 |
| 484.90 | 1.94 | 1.94 |
| 494.94 | 3.81 | 3.81 |
| 494.94 | 4.06 | 4.06 |
| 567.00 | 3.65 | 3.65 |
| 552.97 | 3.26 | 3.26 |
| 568.97 | 2.68 | 2.68 |
| 528.49 | 3.58 | 3.58 |
| 529.38 | 3.87 | 3.87 |
| 512.93 | 3.36 | 3.36 |
| 552.97 | 3.61 | 3.61 |
| 552.97 | 3.02 | 3.02 |
| 567.00 | 3.54 | 3.54 |
| 541.52 | 3.18 | 3.18 |
| 524.96 | 3.23 | 3.23 |
| 483.48 | 3.32 | 3.32 |
| 567.00 | 4.00 | 4.00 |
| 510.93 | 2.92 | 2.92 |
| 538.94 | 2.92 | 2.92 |
| 500.56 | 3.44 | 3.44 |
| 567.99 | 3.11 | 3.11 |
| 547.55 | 3.25 | 3.25 |
| 629.07 | 5.42 | 5.42 |
| 529.38 | 3.87 | 3.87 |
| 494.94 | 3.81 | 3.81 |
| 552.97 | 3.61 | 3.61 |
| 536.59 | 4.23 | 4.23 |
| 537.02 | 4.39 | 4.39 |
| 494.94 | 4.50 | 4.50 |
| 524.96 | 3.69 | 3.69 |
| 512.93 | 3.36 | 3.36 |
| 512.93 | 3.36 | 3.36 |
| 497.51 | 3.71 | 3.71 |
| 485.50 | 3.12 | 3.12 |
| 483.48 | 3.64 | 3.64 |
| 562.93 | 4.24 | 4.24 |
| 611.05 | 3.85 | 3.85 |
| 553.96 | 3.71 | 3.71 |
| 559.00 | 3.67 | 3.67 |
| 496.47 | 2.84 | 2.84 |
| 508.96 | 3.56 | 3.56 |
| 561.58 | 3.63 | 3.63 |
| 571.55 | 2.65 | 2.65 |
| 597.02 | 3.46 | 3.46 |
| 508.96 | 4.13 | 4.13 |