Basic Information
ID: ALA3887660
Type: Binding
Description: Shift AlphaScreen Assay : The AlphaScreen Assay is modified to include the testing of inhibitors against ADAMTS-5 in the presence of 50% Lewis rat plasma in order to determine the effects of plasma protein binding on inhibitor potency. The ratio between the IC50 of the inhibitor against ADAMTS-5 in 50% Lewis rat plasma versus the IC50 of the inhibitor in buffer is calculated and is described as the plasma shift of the inhibitor. The assay is completed in the same manner using 10 nM ADAMTS-5 instead of 2.1 nM. A similar assay is used with dog ADAMTS-4 in the presence of 25% dog plasma.
Format: BAO_0000366
Tissue: Plasma
Document: ALA3886398
BindingDB Source: 7435
Activity Charts
Bioactivity
Activity Types for Assay ALA3887660
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 411.42 | 2.62 | 2.62 |
| 397.40 | 2.38 | 2.38 |
| 409.41 | 2.38 | 2.38 |
| 383.37 | 1.99 | 1.99 |
| 397.40 | 2.38 | 2.38 |