Basic Information
ID: ALA3887673
Type: Binding
Description: Serotonin 5-HT2A Receptor Binding Assay: The assay was performed according to the method by Leysen J E et al. (Leysen J E, Niemegeers C J E, Van Nueten J M and Laduron P M. [3H] Ketanserin (R 41 468), a selective 3H-ligand for serotonin 2 receptor binding sites. Mol. Pharmacol., 1982, 21: 301-314). The binding assay was performed using 40 ul of the membrane specimen, 20 ul of [3H]-Ketanserin (final concentration 1 to 3 nM), 20 ul of a test drug and 50 mM Tris-hydrochloric acid buffer (pH 7.4) so that the total amount was 200 ul (final dimethylsulfoxide concentration 1%). The reaction was performed at 37° C. for 20 minutes and terminated by conducting suction filtration with a cell harvester on a glass fiber filter plate.The filter plate made of glass fiber was washed with 50 mM Tris-hydrochloric acid buffer (pH 7.4), and after dried, a microplate liquid scintillation cocktail was added and the radioactivity was measured with a microplate scintillation counter.
Format: BAO_0000019
Organism: Rattus norvegicus
Target: Serotonin 2a (5-HT2a) receptor(ALA322)
Document: ALA3886400
BindingDB Source: 7437
Activity Charts
Bioactivity
Activity Types for Assay ALA3887673
Ki
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 433.58 | 4.72 | 4.72 |
| 435.59 | 4.77 | 4.77 |
| 439.56 | 4.52 | 4.52 |
| 433.58 | 4.54 | 4.54 |
| 451.57 | 4.86 | 4.86 |
| 447.60 | 5.11 | 5.11 |
| 421.57 | 4.38 | 4.38 |
| 451.59 | 4.00 | 4.00 |
| 433.62 | 5.17 | 5.17 |
| 421.57 | 4.38 | 4.38 |
| 403.55 | 5.04 | 5.04 |
| 447.60 | 4.83 | 4.83 |
| 417.58 | 4.82 | 4.82 |
| 437.57 | 4.08 | 4.08 |
| 435.64 | 4.67 | 4.67 |
| 435.59 | 4.77 | 4.77 |
| 433.62 | 4.89 | 4.89 |
| 447.60 | 5.03 | 5.03 |
| 449.62 | 4.51 | 4.51 |
| 449.62 | 4.79 | 4.79 |
| 421.57 | 4.38 | 4.38 |
| 435.59 | 4.12 | 4.12 |
| 490.46 | 5.68 | 5.68 |
| 447.60 | 4.83 | 4.83 |
| 421.57 | 3.78 | 3.78 |
| 419.59 | 4.78 | 4.78 |
| 419.55 | 4.33 | 4.33 |
| 433.58 | 4.34 | 4.34 |
| 419.55 | 4.33 | 4.33 |
| 433.58 | 4.64 | 4.64 |
| 449.62 | 4.51 | 4.51 |
| 433.58 | 4.64 | 4.64 |
| 433.58 | 4.72 | 4.72 |
| 419.59 | 4.50 | 4.50 |
| 479.60 | 4.30 | 4.30 |
| 435.59 | 4.17 | 4.17 |
| 419.55 | 4.33 | 4.33 |
| 421.57 | 3.78 | 3.78 |
| 433.58 | 4.64 | 4.64 |
| 433.58 | 4.52 | 4.52 |
| 477.67 | 4.92 | 4.92 |
| 449.62 | 4.51 | 4.51 |
| 407.58 | 4.14 | 4.14 |
| 431.61 | 5.21 | 5.21 |
| 481.64 | 4.79 | 4.79 |
| 417.58 | 4.82 | 4.82 |
| 449.62 | 5.16 | 5.16 |
| 437.54 | 4.47 | 4.47 |
| 449.62 | 5.33 | 5.33 |
| 435.59 | 4.12 | 4.12 |
| 451.59 | 3.87 | 3.87 |
| 449.62 | 4.40 | 4.40 |
| 449.62 | 4.79 | 4.79 |
| 447.60 | 5.03 | 5.03 |
| 478.62 | 4.34 | 4.34 |
| 433.58 | 4.34 | 4.34 |
| 435.59 | 4.77 | 4.77 |
| 403.55 | 5.04 | 5.04 |
| 467.61 | 4.45 | 4.45 |
| 405.52 | 3.94 | 3.94 |
| 435.59 | 4.77 | 4.77 |