Basic Information
ID: ALA3887681
Type: Binding
Description: Inhibition Assay: Receptor-expressing HEK 293 cell membrane fraction was analyzed by modifying the methods of C. J. Langmead et al. (see Langmead C J, Szekeres P G; Chambers J K, Ratcliffe S J, Jones D N C, Hirst W D, et al. Characterization of the binding of [125I]-human prolactin releasing peptide (PrRP) to GPR10, a novel G protein coupled receptor. Br J Pharmacol 2000; 131: 683-688.). To each well of a 96-well plate, 150 μl of assay buffer (20 mM HEPES, 10 mM EDTA, 1 μl/ml protease inhibiting agent cocktail, pH 7.4), 20 μl of cell membrane fraction, 10 μl of test compound and [3H]-PrRP (final concentration 1 nM) were added and incubated at room temperature for 90 minutes. After completion of the reaction, using a cell harvester, the cell-membrane fraction sample product was filtered under aspiration by a glass fiber filter plate (Unifilter; GF/B) previously treated with 0.5% polyethylene imine. The filter was washed with a 50 mM Tris hydrochloric acid buffer (pH 7.4) three times.
Cell type: Sf9
Activity Charts
Bioactivity
Activity Types for Assay ALA3887681
Ki
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 481.38 | 4.55 | 4.55 |
| 473.36 | 3.98 | 3.98 |
| 560.58 | 4.84 | 4.84 |
| 537.52 | 4.67 | 4.67 |
| 561.56 | 5.31 | 5.31 |
| 501.41 | 4.62 | 4.62 |
| 538.53 | 4.39 | 4.39 |
| 545.57 | 5.21 | 5.21 |
| 522.57 | 5.27 | 5.27 |
| 524.54 | 4.67 | 4.67 |
| 545.57 | 5.21 | 5.21 |
| 555.38 | 5.30 | 5.30 |
| 498.51 | 4.34 | 4.34 |
| 534.54 | 4.53 | 4.53 |
| 532.95 | 4.51 | 4.51 |
| 477.50 | 2.52 | 2.52 |
| 565.60 | 4.36 | 4.36 |
| 523.49 | 4.28 | 4.28 |
| 514.94 | 4.92 | 4.92 |
| 528.51 | 3.84 | 3.84 |
| 519.96 | 4.48 | 4.48 |
| 486.40 | 4.12 | 4.12 |
| 506.91 | 4.35 | 4.35 |
| 515.47 | 3.70 | 3.70 |
| 560.58 | 4.84 | 4.84 |
| 537.52 | 4.67 | 4.67 |
| 519.53 | 4.53 | 4.53 |
| 528.96 | 5.31 | 5.31 |
| 561.56 | 5.31 | 5.31 |
| 534.54 | 4.30 | 4.30 |
| 563.58 | 4.54 | 4.54 |
| 545.57 | 5.21 | 5.21 |
| 487.39 | 4.37 | 4.37 |
| 459.33 | 3.42 | 3.42 |
| 492.89 | 3.78 | 3.78 |
| 495.99 | 3.94 | 3.94 |
| 528.56 | 4.91 | 4.91 |
| 520.52 | 3.82 | 3.82 |
| 517.42 | 3.17 | 3.17 |
| 506.99 | 3.61 | 3.61 |
| 497.48 | 3.56 | 3.56 |
| 504.48 | 4.14 | 4.14 |
| 518.02 | 4.51 | 4.51 |
| 534.97 | 4.66 | 4.66 |
| 550.06 | 3.87 | 3.87 |
| 500.52 | 4.31 | 4.31 |
| 452.53 | 3.27 | 3.27 |
| 555.56 | 3.60 | 3.60 |
| 525.01 | 3.73 | 3.73 |
| 529.54 | 4.30 | 4.30 |
| 536.94 | 4.11 | 4.11 |
| 558.59 | 4.15 | 4.15 |
| 509.53 | 4.26 | 4.26 |
| 529.49 | 4.09 | 4.09 |
| 527.50 | 3.33 | 3.33 |
| 553.55 | 2.76 | 2.76 |
| 533.94 | 4.43 | 4.43 |
| 492.52 | 4.06 | 4.06 |
| 453.52 | 2.89 | 2.89 |
| 512.94 | 4.41 | 4.41 |
| 533.51 | 3.10 | 3.10 |
| 520.94 | 4.65 | 4.65 |
| 540.47 | 3.54 | 3.54 |
| 444.96 | 3.53 | 3.53 |
| 533.03 | 4.15 | 4.15 |
| 542.51 | 4.45 | 4.45 |
| 557.55 | 4.73 | 4.73 |
| 523.52 | 4.24 | 4.24 |
| 536.94 | 4.35 | 4.35 |
| 546.93 | 5.09 | 5.09 |
| 570.55 | 3.61 | 3.61 |
| 515.49 | 3.78 | 3.78 |
| 553.01 | 4.82 | 4.82 |
| 496.87 | 3.94 | 3.94 |
| 503.39 | 3.75 | 3.75 |
| 516.52 | 4.09 | 4.09 |
| 517.46 | 3.84 | 3.84 |
| 530.50 | 3.46 | 3.46 |
| 550.97 | 3.54 | 3.54 |
| 506.49 | 3.43 | 3.43 |
| 511.50 | 3.95 | 3.95 |
| 556.46 | 4.59 | 4.59 |
| 554.55 | 4.38 | 4.38 |
| 553.54 | 4.74 | 4.74 |
| 520.94 | 4.74 | 4.74 |
| 536.56 | 4.24 | 4.24 |
| 543.57 | 4.69 | 4.69 |
| 529.49 | 4.09 | 4.09 |