Basic Information
ID: ALA3887728
Type: Binding
Description: Radioligand Binding Assay: The CRTH2 receptor binding assay is performed in a scintillation proximity assay (SPA) format with the radioligand [3H]-PGD2 (Perkin Elmer, NET616000MC). CHO-K1-hCRTH2 cell membranes are again homogenized by passing through a single use needle (Terumo, 23G) and diluted in SPA incubation buffer in suitable concentrations (0.5-10 ug protein/well). The SPA assay is set up in 96 well microtiter plates (Perkin Elmer, CatNo. 6005040) in SPA incubation buffer with a final volume of 200 ul per well and final concentration of 50 mM Tris-HCl, 10 mM MgCl2, 150 mM NaCl, 1 mM EDTA pH 7.4, 0.1% bovine serum albumin). The SPA assay mixture contains 60 ul of the membrane suspension, 80 ul of Wheat Germ Agglutinin coated PVT beads (GE Healthcare, RPNQ-0001, 0.3 mg/well), 40 ul of [3H]-PGD2 diluted in SPA buffer to a final concentration of 1 nM (50 000 dpm) and 20 ul of the test compound (dissolved in dimethylsulfoxid).
Format: BAO_0000219
Organism: Homo sapiens
Cell type: CHO-K1-hCRTH2
Target: G protein-coupled receptor 44(ALA5071)
Document: ALA3886426
BindingDB Source: 7462
Activity Charts
Bioactivity
Activity Types for Assay ALA3887728
Ki
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 393.87 | 4.98 | 4.98 |
| 436.94 | 4.89 | 4.89 |
| 374.44 | 3.73 | 3.73 |
| 374.44 | 4.03 | 4.03 |
| 437.48 | 3.77 | 3.77 |
| 394.86 | 4.37 | 4.37 |
| 406.46 | 4.37 | 4.37 |
| 424.45 | 4.50 | 4.50 |
| 441.89 | 4.41 | 4.41 |
| 458.90 | 5.16 | 5.16 |
| 392.43 | 4.17 | 4.17 |
| 402.50 | 4.54 | 4.54 |
| 434.52 | 5.16 | 5.16 |
| 374.44 | 4.03 | 4.03 |
| 439.31 | 4.48 | 4.48 |
| 471.39 | 5.54 | 5.54 |
| 440.91 | 5.02 | 5.02 |
| 393.87 | 4.98 | 4.98 |
| 374.44 | 4.03 | 4.03 |
| 424.45 | 4.50 | 4.50 |
| 444.58 | 5.52 | 5.52 |
| 495.42 | 6.36 | 6.36 |
| 420.49 | 4.67 | 4.67 |
| 361.41 | 3.11 | 3.11 |
| 418.50 | 4.24 | 4.24 |
| 396.40 | 4.00 | 4.00 |
| 440.91 | 5.02 | 5.02 |
| 378.41 | 3.86 | 3.86 |
| 424.45 | 4.50 | 4.50 |
| 440.30 | 3.88 | 3.88 |
| 411.86 | 5.12 | 5.12 |
| 413.48 | 4.10 | 4.10 |
| 412.85 | 4.51 | 4.51 |
| 430.84 | 4.65 | 4.65 |
| 427.43 | 5.34 | 5.34 |
| 379.40 | 3.25 | 3.25 |
| 361.41 | 3.11 | 3.11 |
| 396.40 | 4.00 | 4.00 |
| 403.42 | 3.73 | 3.73 |
| 427.43 | 5.34 | 5.34 |
| 423.90 | 4.28 | 4.28 |
| 422.92 | 4.88 | 4.88 |
| 385.43 | 3.59 | 3.59 |
| 442.44 | 4.64 | 4.64 |
| 408.89 | 4.38 | 4.38 |
| 457.30 | 4.62 | 4.62 |
| 402.50 | 4.24 | 4.24 |
| 427.43 | 5.34 | 5.34 |
| 410.48 | 4.87 | 4.87 |
| 360.42 | 3.72 | 3.72 |
| 429.31 | 5.03 | 5.03 |
| 388.47 | 4.23 | 4.23 |
| 408.43 | 3.87 | 3.87 |
| 407.45 | 3.76 | 3.76 |
| 392.43 | 4.17 | 4.17 |
| 447.30 | 5.16 | 5.16 |
| 378.41 | 3.86 | 3.86 |
| 395.85 | 3.77 | 3.77 |
| 413.84 | 3.91 | 3.91 |
| 457.36 | 5.53 | 5.53 |
| 409.42 | 3.26 | 3.26 |
| 438.53 | 5.38 | 5.38 |
| 396.40 | 4.00 | 4.00 |
| 389.46 | 3.62 | 3.62 |
| 428.41 | 4.74 | 4.74 |
| 436.94 | 5.19 | 5.19 |
| 442.44 | 4.64 | 4.64 |
| 406.46 | 4.37 | 4.37 |
| 416.53 | 5.02 | 5.02 |
| 468.36 | 4.38 | 4.38 |
| 457.36 | 5.53 | 5.53 |
| 414.39 | 4.14 | 4.14 |
| 407.45 | 3.76 | 3.76 |
| 396.40 | 4.00 | 4.00 |
| 486.35 | 4.52 | 4.52 |
| 378.41 | 3.86 | 3.86 |
| 390.45 | 3.02 | 3.02 |
| 402.50 | 4.54 | 4.54 |
| 390.44 | 3.73 | 3.73 |
| 393.87 | 4.98 | 4.98 |
| 436.49 | 4.37 | 4.37 |
| 393.87 | 4.98 | 4.98 |
| 469.37 | 5.85 | 5.85 |
| 456.47 | 5.25 | 5.25 |
| 360.42 | 3.72 | 3.72 |
| 429.31 | 5.03 | 5.03 |
| 462.57 | 5.66 | 5.66 |
| 501.81 | 5.64 | 5.64 |
| 485.36 | 5.13 | 5.13 |
| 431.47 | 4.24 | 4.24 |
| 445.42 | 5.48 | 5.48 |
| 389.46 | 3.62 | 3.62 |
| 393.42 | 3.56 | 3.56 |
| 408.89 | 4.68 | 4.68 |
| 424.45 | 4.50 | 4.50 |