Assay Report Card

Basic Information

ID: ALA3887729

Type: Binding

Description: Enzyme Assay: Human aminopeptidase P2 (APP2) enzyme activity was determined using the internally quenched fluorescent substrate: H-Lys(2-aminobenzoyl)-Pro-Pro-p-nitroanilide (Bachem, Torrance, Calif.) at 1 μM (=Km) for inhibitor studies and 5 μM for enzyme purification assays. The assay buffer was 0.1 M HEPES, pH 7.4, and the assay temperature was 30° C. Cleavage of the substrate by the enzyme produces an increase in fluorescence due to the release of H-Lys(2-aminobenzoyl). Enzyme rates were determined in an F. fluorescence plate reader (Molecular Devices, Sunnyvale, Calif.) using an excitation wavelength of 320 nm and an emission wavelength of 405 nm.

Activity Charts

Compound Summary

Parent Molecular WeightALogPPolar Surface Area
439.581.391.39
427.571.241.24
439.581.251.25
457.571.191.19