ID: ALA3887730
Type: Binding
Description: Enzyme Assay: Human recombinant angiotensin-converting enzyme (hrACE) was obtained from R&D Systems (Minneapolis, Minn.). Its enzyme activity was determined using the internally quenched fluorescent substrate ES005: (7-methoxycoumarin-4-yl)acetyl-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-(2,4-dinitrophenyl)-Lys (R&D Systems, Minneapolis, Minn.). The assay buffer was 0.1 M HEPES, pH 7.4, and the temperature was 30° C. The substrate concentration was below Km (≦40 μM). Enzyme rates were determined in an Fmax fluorescence plate reader (Molecular Devices, Sunnyvale, Calif.) using an excitation wavelength of 320 nm and an emission wavelength of 405 nm.
Organism: Homo sapiens
Bioactivity
Activity Types for Assay ALA3887730
IC50
Parent Molecular Weight | ALogP | Polar Surface Area |
---|---|---|
439.58 | 1.39 | 1.39 |
427.57 | 1.24 | 1.24 |
439.58 | 1.25 | 1.25 |
457.57 | 1.19 | 1.19 |