Basic Information
ID: ALA3887784
Type: Binding
Description: Endothelial Lipase Assay: To assay for cell surface lipase activity, cells expressing human endothelial lipase (EL) or LPL were plated in CellBIND® 384-well plates (Corning, Lowell, Mass.) in 25 μL serum free medium at a density of 2000 cells/well. After 18-24 hours incubation at 37° C., the medium was removed and replaced with 15 μL assay buffer [Hank's Buffered Saline Solution with 25 mM HEPES pH 7.2] and 15 μL PLA1 substrate for a final concentration of 10 μM using a Multidrop reagent dispenser. Fluorescence signal was monitored for 30 min at 37° C. on a Safire II plate reader in kinetic mode (60 cycles, kinetic interval: 30 seconds) with an excitation wavelength of 490 nm and an emission wavelength of 515 nm.
Organism: Homo sapiens
Cell type: 2000
Activity Charts
Bioactivity
Activity Types for Assay ALA3887784
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 331.34 | 4.84 | 4.84 |
| 361.43 | 5.94 | 5.94 |
| 359.39 | 5.40 | 5.40 |
| 315.34 | 5.20 | 5.20 |
| 345.36 | 5.23 | 5.23 |
| 357.42 | 6.17 | 6.17 |
| 374.41 | 5.20 | 5.20 |
| 423.46 | 3.46 | 3.46 |
| 454.41 | 5.74 | 5.74 |
| 401.43 | 3.75 | 3.75 |
| 429.49 | 5.38 | 5.38 |
| 429.49 | 4.35 | 4.35 |
| 329.37 | 5.59 | 5.59 |
| 343.39 | 5.78 | 5.78 |
| 329.37 | 5.39 | 5.39 |
| 388.43 | 4.25 | 4.25 |
| 329.37 | 5.39 | 5.39 |
| 444.45 | 4.35 | 4.35 |
| 361.36 | 5.06 | 5.06 |
| 386.42 | 4.15 | 4.15 |
| 344.38 | 4.55 | 4.55 |
| 444.50 | 6.20 | 6.20 |
| 463.50 | 6.49 | 6.49 |
| 345.36 | 5.10 | 5.10 |
| 482.47 | 6.38 | 6.38 |
| 422.47 | 4.22 | 4.22 |
| 343.39 | 5.78 | 5.78 |