Basic Information
ID: ALA3887840
Type: Binding
Description: Kinase Assay (Method A): The kinase activity of the bi-functional enzyme is readily quantified based on the production of F-2,6-P2 as described by Van Schaftingen et al. (1982) Eur. J. Biochem. 129, 191-5. This sensitive assay is based on the potent activation of pyrophosphate dependent phosphofructokinase-1 (PPi-PFK) from potato tubers by F-2,6-P2. The use of a series of coupled enzymes leads to a consumption of NADH (nicotinamide adenine dinucleotide) that can be followed spectrophotometrically (an updated protocol is available in Van Schaftingen, (1984) Methods of Enzymatic Analysis (Bergmeyer, H. U., ed.), 3rd edn., vol. 6, pp. 335-341, Verlag Chemie, Weinheim). A protocol for measurements in 96-well microtiter plate format is also available (Bruni et al., (1989) Anal. Biochem. 178, 324-6). These assay protocols have been adopted for the measurement of the kinase activity of PFKFB3 with some modifications as described in the protocol below. The assay was run in 96-well microtiter plates.
Format: BAO_0000357
Target: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3(ALA2331053)
Document: ALA3886470
BindingDB Source: 7548
Activity Charts
Bioactivity
Activity Types for Assay ALA3887840
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 404.67 | 3.63 | 3.63 |
| 374.41 | 2.38 | 2.38 |
| 389.50 | 4.83 | 4.83 |
| 410.54 | 4.60 | 4.60 |
| 390.49 | 4.23 | 4.23 |
| 390.49 | 4.23 | 4.23 |
| 434.50 | 4.74 | 4.74 |
| 471.56 | 3.87 | 3.87 |
| 397.46 | 3.85 | 3.85 |
| 373.43 | 4.36 | 4.36 |
| 403.53 | 4.76 | 4.76 |
| 395.42 | 3.47 | 3.47 |
| 407.45 | 3.34 | 3.34 |
| 351.39 | 2.82 | 2.82 |
| 404.50 | 3.16 | 3.16 |
| 377.43 | 3.33 | 3.33 |
| 381.86 | 4.36 | 4.36 |
| 386.46 | 2.77 | 2.77 |
| 404.47 | 3.67 | 3.67 |
| 446.32 | 4.64 | 4.64 |
| 384.25 | 3.28 | 3.28 |
| 445.43 | 4.35 | 4.35 |
| 448.22 | 3.45 | 3.45 |
| 426.48 | 2.49 | 2.49 |
| 425.91 | 2.90 | 2.90 |
| 360.42 | 3.31 | 3.31 |
| 350.38 | 2.90 | 2.90 |
| 353.77 | 2.46 | 2.46 |
| 368.38 | 2.32 | 2.32 |