Basic Information
ID: ALA3887848
Type: Binding
Description: MGAT LCMS Assay: The MGAT enzyme reactions were performed in CORNING Falcon 96-well Polypropylene plates, in a total volume of 60 uL of 50 mM Potassium Phosphate buffer pH 7.4, containing a final concentration of 100 uM 2-oleoylglycerol, 15 uM oleoyl-Coenzyme A and 0.0013 ug/uL Human or Mouse MGAT-2 or 0.0026 ug/uL Rat recombinant MGAT-2 membranes expressed in Sf9 cells. Assay plates were run through a fully automated robotics system and shaken for 5 seconds every minute for a total 10 minutes. The reactions were then quenched with 120 uL of ice cold methanol containing 1 ug/mL 1,2-distearoyl-rac-glycerol as the internal standard. Plates were shaken for 2 minutes and spun down to remove protein precipitation. After the spin, samples were transferred to LC/MS compatible PCR plates. For LC/MS analysis, a ThermoFisher Surveyor pump, utilizing a Waters Symmetry C8, 50x2.1 mm column, was used for the chromatography of enzyme products.
Cell type: Sf9
Activity Charts
Bioactivity
Activity Types for Assay ALA3887848
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 553.51 | 5.61 | 5.61 |
| 606.56 | 7.09 | 7.09 |
| 539.48 | 5.43 | 5.43 |
| 428.45 | 5.43 | 5.43 |
| 620.59 | 7.40 | 7.40 |
| 523.48 | 6.26 | 6.26 |
| 512.45 | 5.98 | 5.98 |
| 511.43 | 4.73 | 4.73 |
| 554.53 | 6.12 | 6.12 |
| 546.52 | 5.96 | 5.96 |
| 442.48 | 5.67 | 5.67 |
| 522.53 | 4.84 | 4.84 |
| 606.56 | 7.09 | 7.09 |
| 520.83 | 6.46 | 6.46 |
| 601.52 | 6.13 | 6.13 |
| 658.56 | 4.90 | 4.90 |
| 559.44 | 4.89 | 4.89 |
| 601.52 | 6.13 | 6.13 |
| 581.51 | 6.13 | 6.13 |
| 606.56 | 7.44 | 7.44 |
| 468.52 | 6.21 | 6.21 |
| 617.88 | 8.27 | 8.27 |
| 575.46 | 5.75 | 5.75 |
| 577.53 | 6.23 | 6.23 |
| 610.98 | 7.49 | 7.49 |
| 624.55 | 7.23 | 7.23 |
| 555.44 | 4.54 | 4.54 |
| 541.45 | 4.75 | 4.75 |
| 512.45 | 5.98 | 5.98 |
| 296.41 | 3.99 | 3.99 |
| 502.41 | 5.68 | 5.68 |
| 540.50 | 5.73 | 5.73 |
| 470.42 | 5.18 | 5.18 |
| 543.44 | 5.26 | 5.26 |
| 562.52 | 6.09 | 6.09 |
| 597.51 | 5.83 | 5.83 |
| 555.48 | 4.96 | 4.96 |
| 525.45 | 6.61 | 6.61 |
| 607.67 | 6.68 | 6.68 |
| 505.66 | 5.15 | 5.15 |
| 542.62 | 4.14 | 4.14 |
| 576.54 | 7.09 | 7.09 |
| 537.29 | 6.97 | 6.97 |
| 581.40 | 5.32 | 5.32 |
| 483.49 | 4.58 | 4.58 |
| 568.48 | 4.35 | 4.35 |
| 530.44 | 6.20 | 6.20 |
| 592.56 | 4.57 | 4.57 |
| 571.50 | 6.04 | 6.04 |
| 519.47 | 4.82 | 4.82 |
| 508.50 | 4.45 | 4.45 |
| 643.58 | 7.24 | 7.24 |
| 539.48 | 5.51 | 5.51 |
| 606.56 | 7.09 | 7.09 |
| 514.47 | 5.19 | 5.19 |
| 562.52 | 6.09 | 6.09 |
| 551.49 | 5.69 | 5.69 |
| 555.58 | 5.95 | 5.95 |
| 666.53 | 6.12 | 6.12 |
| 553.46 | 4.74 | 4.74 |
| 623.48 | 6.33 | 6.33 |
| 590.56 | 7.14 | 7.14 |
| 594.56 | 6.05 | 6.05 |
| 491.63 | 4.76 | 4.76 |
| 416.45 | 4.64 | 4.64 |
| 462.47 | 5.93 | 5.93 |
| 662.55 | 7.33 | 7.33 |
| 545.87 | 5.46 | 5.46 |
| 604.59 | 6.81 | 6.81 |
| 577.43 | 5.41 | 5.41 |
| 493.41 | 5.53 | 5.53 |
| 577.53 | 6.15 | 6.15 |
| 577.49 | 5.00 | 5.00 |
| 524.56 | 3.63 | 3.63 |
| 472.51 | 5.44 | 5.44 |
| 578.55 | 6.88 | 6.88 |
| 519.64 | 4.08 | 4.08 |
| 569.51 | 5.60 | 5.60 |
| 612.52 | 7.25 | 7.25 |
| 450.46 | 4.48 | 4.48 |
| 538.59 | 7.24 | 7.24 |
| 524.46 | 6.06 | 6.06 |
| 608.58 | 7.04 | 7.04 |
| 564.57 | 4.34 | 4.34 |
| 543.55 | 4.96 | 4.96 |
| 639.49 | 6.83 | 6.83 |
| 561.43 | 5.29 | 5.29 |
| 482.42 | 5.97 | 5.97 |
| 504.58 | 5.37 | 5.37 |
| 639.49 | 6.83 | 6.83 |
| 582.59 | 6.90 | 6.90 |
| 628.97 | 7.63 | 7.63 |
| 567.49 | 5.82 | 5.82 |
| 646.51 | 7.59 | 7.59 |
| 374.48 | 4.89 | 4.89 |
| 390.53 | 4.87 | 4.87 |
| 570.57 | 6.75 | 6.75 |
| 642.54 | 7.09 | 7.09 |
| 467.45 | 3.58 | 3.58 |
| 468.40 | 5.66 | 5.66 |
| 610.98 | 7.74 | 7.74 |
| 512.45 | 5.81 | 5.81 |
| 529.52 | 4.98 | 4.98 |
| 610.98 | 7.49 | 7.49 |
| 525.45 | 5.12 | 5.12 |
| 511.43 | 4.81 | 4.81 |
| 433.43 | 3.38 | 3.38 |
| 608.56 | 4.27 | 4.27 |
| 498.42 | 5.67 | 5.67 |
| 576.54 | 7.00 | 7.00 |
| 570.38 | 6.70 | 6.70 |
| 511.62 | 4.42 | 4.42 |
| 488.51 | 3.83 | 3.83 |
| 513.40 | 4.04 | 4.04 |
| 513.58 | 3.17 | 3.17 |
| 417.44 | 3.82 | 3.82 |
| 568.48 | 4.65 | 4.65 |
| 628.97 | 7.63 | 7.63 |
| 645.56 | 5.66 | 5.66 |
| 386.38 | 3.26 | 3.26 |
| 554.45 | 4.21 | 4.21 |
| 478.27 | 4.16 | 4.16 |
| 582.50 | 5.53 | 5.53 |
| 543.44 | 5.26 | 5.26 |
| 542.48 | 5.11 | 5.11 |
| 468.40 | 5.58 | 5.58 |
| 554.50 | 4.88 | 4.88 |
| 607.53 | 6.88 | 6.88 |
| 607.55 | 6.49 | 6.49 |
| 567.53 | 6.21 | 6.21 |
| 518.46 | 6.82 | 6.82 |
| 606.56 | 7.09 | 7.09 |
| 678.03 | 8.14 | 8.14 |
| 619.47 | 6.12 | 6.12 |
| 612.96 | 6.28 | 6.28 |
| 583.51 | 5.90 | 5.90 |
| 553.46 | 5.68 | 5.68 |
| 607.53 | 6.88 | 6.88 |
| 416.45 | 4.43 | 4.43 |
| 510.48 | 6.75 | 6.75 |
| 472.43 | 5.36 | 5.36 |
| 576.54 | 7.00 | 7.00 |
| 539.48 | 5.43 | 5.43 |
| 619.51 | 6.23 | 6.23 |
| 502.84 | 6.32 | 6.32 |
| 454.37 | 5.19 | 5.19 |
| 543.46 | 5.61 | 5.61 |
| 572.61 | 4.42 | 4.42 |
| 580.53 | 5.82 | 5.82 |
| 381.52 | 4.03 | 4.03 |
| 519.57 | 5.72 | 5.72 |
| 555.48 | 5.21 | 5.21 |
| 512.45 | 6.06 | 6.06 |
| 600.51 | 7.25 | 7.25 |
| 623.48 | 6.33 | 6.33 |
| 622.44 | 6.10 | 6.10 |
| 543.51 | 5.72 | 5.72 |
| 608.55 | 7.53 | 7.53 |
| 549.51 | 7.48 | 7.48 |
| 505.54 | 5.33 | 5.33 |
| 483.57 | 3.99 | 3.99 |
| 598.46 | 4.33 | 4.33 |
| 574.57 | 7.08 | 7.08 |
| 496.45 | 6.28 | 6.28 |
| 628.52 | 6.70 | 6.70 |
| 624.55 | 7.23 | 7.23 |
| 581.51 | 6.38 | 6.38 |
| 377.49 | 4.82 | 4.82 |
| 433.43 | 5.31 | 5.31 |
| 500.41 | 6.11 | 6.11 |
| 646.38 | 6.59 | 6.59 |
| 534.40 | 6.26 | 6.26 |
| 546.49 | 5.63 | 5.63 |
| 610.98 | 7.74 | 7.74 |
| 497.40 | 4.34 | 4.34 |
| 559.44 | 4.96 | 4.96 |
| 609.51 | 6.06 | 6.06 |
| 577.43 | 5.34 | 5.34 |
| 581.52 | 5.31 | 5.31 |
| 604.54 | 6.75 | 6.75 |
| 525.45 | 5.12 | 5.12 |
| 518.40 | 6.22 | 6.22 |
| 629.50 | 6.52 | 6.52 |
| 608.54 | 5.63 | 5.63 |
| 502.84 | 6.32 | 6.32 |
| 660.53 | 7.98 | 7.98 |
| 516.87 | 6.62 | 6.62 |
| 503.52 | 4.13 | 4.13 |
| 498.42 | 5.67 | 5.67 |
| 552.39 | 6.56 | 6.56 |
| 516.87 | 6.62 | 6.62 |
| 617.49 | 6.38 | 6.38 |
| 545.87 | 5.46 | 5.46 |
| 607.57 | 4.07 | 4.07 |
| 594.53 | 6.97 | 6.97 |
| 486.39 | 5.80 | 5.80 |
| 541.45 | 4.82 | 4.82 |
| 386.37 | 4.26 | 4.26 |
| 422.35 | 4.50 | 4.50 |
| 656.57 | 7.48 | 7.48 |
| 644.53 | 8.11 | 8.11 |
| 525.45 | 5.12 | 5.12 |
| 557.47 | 5.65 | 5.65 |
| 509.49 | 3.65 | 3.65 |
| 578.51 | 6.31 | 6.31 |
| 444.50 | 5.21 | 5.21 |
| 536.39 | 6.68 | 6.68 |
| 628.52 | 6.95 | 6.95 |
| 595.42 | 5.71 | 5.71 |
| 483.53 | 5.33 | 5.33 |
| 498.48 | 5.51 | 5.51 |
| 597.51 | 5.83 | 5.83 |
| 593.45 | 7.01 | 7.01 |
| 673.61 | 7.40 | 7.40 |
| 567.49 | 5.82 | 5.82 |
| 590.56 | 7.39 | 7.39 |
| 546.90 | 6.71 | 6.71 |
| 538.49 | 5.96 | 5.96 |
| 565.52 | 5.69 | 5.69 |
| 581.52 | 4.96 | 4.96 |
| 516.41 | 5.81 | 5.81 |
| 587.50 | 5.49 | 5.49 |
| 622.57 | 6.28 | 6.28 |
| 578.51 | 5.63 | 5.63 |
| 579.42 | 5.83 | 5.83 |
| 569.46 | 4.58 | 4.58 |
| 629.50 | 6.77 | 6.77 |
| 553.51 | 5.90 | 5.90 |
| 532.87 | 6.32 | 6.32 |
| 566.50 | 5.73 | 5.73 |
| 516.41 | 5.62 | 5.62 |
| 449.55 | 3.45 | 3.45 |
| 426.48 | 5.98 | 5.98 |
| 543.44 | 5.26 | 5.26 |
| 528.45 | 5.68 | 5.68 |
| 644.56 | 7.03 | 7.03 |
| 560.55 | 7.30 | 7.30 |
| 541.45 | 4.82 | 4.82 |
| 590.32 | 5.57 | 5.57 |
| 650.55 | 6.79 | 6.79 |
| 525.65 | 4.81 | 4.81 |
| 592.54 | 6.79 | 6.79 |
| 593.45 | 5.91 | 5.91 |
| 561.49 | 5.96 | 5.96 |
| 513.45 | 4.33 | 4.33 |
| 539.68 | 5.75 | 5.75 |
| 608.58 | 6.44 | 6.44 |
| 493.41 | 5.53 | 5.53 |
| 596.53 | 5.38 | 5.38 |
| 580.53 | 5.57 | 5.57 |
| 490.53 | 6.26 | 6.26 |
| 561.50 | 6.22 | 6.22 |
| 661.57 | 7.63 | 7.63 |
| 538.57 | 6.13 | 6.13 |
| 516.41 | 5.81 | 5.81 |
| 416.45 | 4.28 | 4.28 |
| 617.51 | 8.50 | 8.50 |
| 507.46 | 4.82 | 4.82 |
| 516.41 | 5.62 | 5.62 |
| 608.58 | 7.04 | 7.04 |
| 525.45 | 5.12 | 5.12 |
| 544.49 | 4.90 | 4.90 |