Basic Information
ID: ALA3887914
Type: Binding
Description: Enzyme Assay: The enzymatic assay was measured using white, opaque 96-well half area plates. Each well contained 60 uL of assay buffer (50 mM HEPES pH 8.0, 50 mM NaCl and 1 mM CaCl2) and 2 ul of a DMSO solution containing compound of interest. Conditioned media obtained from HT-1080 cells, which were transformed by RAGE technology (Athersys) to overexpress endogenous EL, was added and the reaction was allowed to incubate for 20 min at 37° C. with gentle agitation. The reaction was started by the addition of 20 uL of a 1:4 dilution of vesicles. The final total reaction volume was 100 uL. The reaction rates were measured on a Gemini plate reader with an excitation wavelength of 488 nm and a emission of 530 nm. Readings were taken every 20 seconds for 10 min with agitation between each reading.
Organism: Homo sapiens
Cell type: HT-1080
Activity Charts
Bioactivity
Activity Types for Assay ALA3887914
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 419.31 | 3.90 | 3.90 |
| 447.36 | 4.33 | 4.33 |
| 329.18 | 1.94 | 1.94 |
| 385.29 | 3.35 | 3.35 |
| 401.29 | 2.73 | 2.73 |
| 451.33 | 4.08 | 4.08 |
| 357.24 | 2.71 | 2.71 |
| 463.32 | 3.59 | 3.59 |
| 294.74 | 1.28 | 1.28 |
| 467.78 | 4.59 | 4.59 |
| 419.31 | 4.07 | 4.07 |
| 260.29 | 0.63 | 0.63 |
| 319.36 | 0.81 | 0.81 |
| 342.32 | 2.04 | 2.04 |
| 447.36 | 4.25 | 4.25 |
| 415.32 | 3.12 | 3.12 |
| 399.32 | 3.74 | 3.74 |
| 437.30 | 4.04 | 4.04 |
| 433.34 | 3.94 | 3.94 |
| 343.21 | 2.33 | 2.33 |
| 437.30 | 4.04 | 4.04 |
| 453.75 | 4.55 | 4.55 |
| 385.29 | 3.50 | 3.50 |
| 488.20 | 5.20 | 5.20 |
| 383.27 | 3.25 | 3.25 |
| 399.32 | 3.74 | 3.74 |
| 274.32 | 1.02 | 1.02 |
| 385.29 | 3.35 | 3.35 |
| 465.35 | 4.47 | 4.47 |
| 413.35 | 4.13 | 4.13 |