Basic Information
ID: ALA3887945
Type: Binding
Description: Radioligand Displacement Assay: Binding assay was performed in a final assay volume of 250 ul. First, 25 ul of test compound, previously diluted in Binding-Buffer (Binding-Buffer: 50 mM Tris-Base, 100 mM NaCl, 1 mM EDTA, 0.1% BSA (protease free), 0.01% NaN3, 10 mM MnCl2, pH 7.0) was placed into each well. After addition of 75 ul Binding-Buffer, 50 ul of the radioligand 3H-PGD2 (at 2.5 nM (220.000 dpm/well) from ANAWA ART0662) was added to each well. Binding assay was started by addition of 100 ul CRTH2 membrane fragments, reaching a final concentration of 20 ug/well. For non-specific binding, PGD2 was added to the reaction mixture to 10 mM final concentration. This assay mix was incubated for 90 minutes at room temperature and then filtered through a GF/C filter 96-well plate which was pre-soaked for 3 hours in 0.5% polyethyleneimine (PEI). The filter-wells were washed three times with ice cold Binding-Buffer. Then, 40 ul of Microscint-40 (Packard) was added to each well and the retained radioactivity quantified in a Topcount (Packard).
Format: BAO_0000019
Organism: Homo sapiens
Target: G protein-coupled receptor 44(ALA5071)
Document: ALA3886512
BindingDB Source: 7610
Activity Charts
Bioactivity
Activity Types for Assay ALA3887945
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 449.48 | 4.82 | 4.82 |
| 419.45 | 4.81 | 4.81 |
| 495.98 | 5.69 | 5.69 |
| 449.48 | 4.82 | 4.82 |
| 502.57 | 5.34 | 5.34 |
| 461.53 | 5.04 | 5.04 |
| 477.51 | 4.96 | 4.96 |
| 509.52 | 5.70 | 5.70 |
| 488.56 | 4.47 | 4.47 |
| 497.95 | 4.88 | 4.88 |
| 445.49 | 4.77 | 4.77 |
| 463.51 | 3.77 | 3.77 |
| 437.44 | 4.95 | 4.95 |
| 510.42 | 5.98 | 5.98 |
| 463.96 | 5.16 | 5.16 |
| 459.52 | 4.82 | 4.82 |
| 459.52 | 4.82 | 4.82 |
| 473.54 | 5.13 | 5.13 |
| 509.58 | 4.09 | 4.09 |
| 497.54 | 4.22 | 4.22 |
| 467.47 | 4.96 | 4.96 |
| 507.58 | 4.21 | 4.21 |
| 401.43 | 3.05 | 3.05 |
| 419.45 | 4.81 | 4.81 |
| 502.59 | 4.86 | 4.86 |
| 517.03 | 5.95 | 5.95 |
| 500.57 | 5.44 | 5.44 |
| 437.44 | 4.95 | 4.95 |
| 516.57 | 5.14 | 5.14 |
| 449.48 | 4.82 | 4.82 |
| 487.57 | 5.60 | 5.60 |
| 505.01 | 4.98 | 4.98 |
| 493.96 | 5.47 | 5.47 |
| 477.53 | 5.60 | 5.60 |
| 467.47 | 4.96 | 4.96 |
| 477.51 | 4.96 | 4.96 |
| 467.47 | 4.23 | 4.23 |
| 477.51 | 4.96 | 4.96 |
| 480.56 | 4.80 | 4.80 |
| 435.45 | 4.78 | 4.78 |
| 419.45 | 4.81 | 4.81 |
| 481.50 | 4.62 | 4.62 |
| 449.48 | 4.82 | 4.82 |
| 459.52 | 4.82 | 4.82 |
| 462.52 | 4.35 | 4.35 |
| 459.52 | 4.82 | 4.82 |
| 383.42 | 3.42 | 3.42 |
| 453.90 | 5.47 | 5.47 |
| 433.48 | 5.12 | 5.12 |
| 513.49 | 5.71 | 5.71 |
| 477.51 | 4.96 | 4.96 |
| 500.57 | 5.37 | 5.37 |
| 439.53 | 4.70 | 4.70 |
| 453.90 | 5.47 | 5.47 |
| 447.51 | 4.65 | 4.65 |
| 500.57 | 5.14 | 5.14 |
| 453.90 | 5.47 | 5.47 |
| 558.05 | 6.89 | 6.89 |
| 493.96 | 5.47 | 5.47 |
| 461.53 | 4.95 | 4.95 |
| 463.94 | 5.46 | 5.46 |
| 493.96 | 5.47 | 5.47 |
| 463.51 | 4.87 | 4.87 |
| 527.51 | 5.84 | 5.84 |
| 493.96 | 5.47 | 5.47 |
| 449.48 | 3.44 | 3.44 |
| 475.97 | 5.33 | 5.33 |
| 448.49 | 4.04 | 4.04 |
| 476.50 | 4.13 | 4.13 |
| 448.49 | 4.04 | 4.04 |
| 487.45 | 5.83 | 5.83 |
| 528.41 | 6.12 | 6.12 |
| 514.41 | 5.40 | 5.40 |
| 459.52 | 4.82 | 4.82 |
| 477.51 | 4.96 | 4.96 |
| 504.51 | 4.41 | 4.41 |
| 463.51 | 4.48 | 4.48 |
| 473.54 | 4.86 | 4.86 |
| 429.49 | 3.83 | 3.83 |
| 477.51 | 4.96 | 4.96 |
| 411.47 | 4.06 | 4.06 |
| 433.43 | 4.30 | 4.30 |
| 446.48 | 4.12 | 4.12 |
| 489.54 | 4.83 | 4.83 |
| 517.03 | 5.65 | 5.65 |
| 453.90 | 5.47 | 5.47 |
| 493.96 | 5.47 | 5.47 |
| 419.45 | 4.81 | 4.81 |
| 479.52 | 5.18 | 5.18 |
| 503.45 | 5.71 | 5.71 |
| 447.51 | 4.65 | 4.65 |
| 449.48 | 4.09 | 4.09 |
| 450.47 | 3.49 | 3.49 |
| 437.44 | 4.95 | 4.95 |
| 519.04 | 5.37 | 5.37 |
| 437.44 | 4.95 | 4.95 |
| 459.52 | 4.82 | 4.82 |
| 503.99 | 4.97 | 4.97 |
| 445.49 | 4.73 | 4.73 |
| 487.53 | 4.45 | 4.45 |