Basic Information
ID: ALA3887964
Type: Binding
Description: In vitro IRAK-1 and IRAK-4 Assay: Purified recombinant IRAK-4 protein was incubated with 250 uM synthetic peptide(KKARFSRFAGSSPSQSSMVAR) in 30 ul of kinase buffer including (20 mM MOPS pH7.2, 25 mM beta glycerol phosphate, 5 mM EGTA, 1 mM sodium orthovanadate, 1 mM DTT, 50 uM ATP, 20 mM MgCl2, 10 uCi gamma-33P, 0.1% BSA) for the indicated time. For purified recombinant IRAK-1 protein kinase assay, 50 uM ATP was used. A 25 ul aliquot of the reaction mixture was transferred on to p81 phosphocellulose squares (Upstate Biotechnology, Lake Placid, N.Y.). The assay squares were washed three times with 0.75% phosphoric acid and once with acetone. Enzyme activity was measured by determining the bound radioactivity by liquid scintillation counting.
Activity Charts
Bioactivity
Activity Types for Assay ALA3887964
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 400.87 | 2.52 | 2.52 |
| 408.49 | 2.90 | 2.90 |
| 385.86 | 2.84 | 2.84 |
| 399.88 | 3.23 | 3.23 |
| 408.48 | 4.03 | 4.03 |
| 402.91 | 3.81 | 3.81 |
| 425.47 | 2.27 | 2.27 |
| 403.89 | 4.28 | 4.28 |
| 387.89 | 4.33 | 4.33 |
| 420.49 | 4.17 | 4.17 |
| 391.46 | 4.07 | 4.07 |
| 395.44 | 2.90 | 2.90 |
| 469.55 | 3.41 | 3.41 |
| 409.47 | 2.93 | 2.93 |
| 372.82 | 2.73 | 2.73 |
| 402.45 | 3.82 | 3.82 |
| 402.84 | 3.02 | 3.02 |
| 403.89 | 4.28 | 4.28 |
| 377.86 | 2.98 | 2.98 |
| 394.46 | 2.87 | 2.87 |
| 401.92 | 3.77 | 3.77 |
| 401.85 | 2.55 | 2.55 |
| 418.53 | 3.67 | 3.67 |
| 408.48 | 4.03 | 4.03 |
| 384.87 | 2.81 | 2.81 |
| 420.49 | 4.17 | 4.17 |
| 428.92 | 3.12 | 3.12 |
| 420.49 | 4.17 | 4.17 |
| 335.32 | 2.24 | 2.24 |
| 379.45 | 3.22 | 3.22 |
| 406.47 | 3.78 | 3.78 |