Basic Information
ID: ALA3887991
Type: Binding
Description: PARP-2 and PARP-3 Enzymatic Assay: PARP-2 and PARP-3 enzymatic assays were conducted using commercial PARP-2/PARP-3 Chemiluminescent Assay Kit (BPS Biosciences) and the protocols with the kits. Briefly, histones were coated in a high binding plate first, and incubated with PARP-2 or PARP-3, and increasing concentrations of compounds for 0.5 h. Then, biotinylated NAD and activated DNA were added to the wells. The biotinylated PARylation product was measured by adding streptavidin-HRP and HRP substrates which produce chemiluminescence.
Activity Charts
Bioactivity
Activity Types for Assay ALA3887991
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 225.25 | 1.95 | 1.95 |
| 268.32 | 1.84 | 1.84 |
| 271.30 | 2.73 | 2.73 |
| 294.31 | 1.37 | 1.37 |
| 352.48 | 3.40 | 3.40 |
| 309.37 | 2.46 | 2.46 |
| 311.35 | 0.69 | 0.69 |
| 310.36 | 2.00 | 2.00 |
| 364.49 | 3.55 | 3.55 |
| 298.32 | 2.08 | 2.08 |