Basic Information
ID: ALA3887994
Type: Binding
Description: ALK-5 Gene : ALK-5 gene reporter assay methods have been described in the art (see e.g., Maliekal et al. (2004) J Biol Chem 279(35):36287-36292). The compounds named in the specified Examples were tested as follows for inhibition of Smad binding element (SBE) luciferase reporter activity in TGFbeta1 stimulated NIH-3T3 cells. The following luciferase assay employs NIH/3T3 (murine fibroblast) cells, which are transiently transfected with a Smad binding element (SBE) luciferase reporter construct. This expressed construct is responsive to agents that stimulate the Smad signaling pathway.
Organism: Homo sapiens
Cell type: NIH3T3
Activity Charts
Bioactivity
Activity Types for Assay ALA3887994
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 292.37 | 3.85 | 3.85 |
| 306.39 | 4.16 | 4.16 |
| 320.42 | 4.41 | 4.41 |
| 320.42 | 4.41 | 4.41 |
| 332.43 | 4.34 | 4.34 |
| 320.42 | 4.47 | 4.47 |
| 306.39 | 4.16 | 4.16 |
| 306.39 | 4.16 | 4.16 |
| 332.43 | 4.34 | 4.34 |