Assay Report Card

Basic Information

ID: ALA3888094

Type: Binding

Description: Fluorescence Polarization Assays: In another embodiment, Fluorescence Polarization ("FP") based assays may be used to detect and monitor ligand binding. Fluorescence polarization is a versatile laboratory technique for measuring equilibrium binding, nucleic acid hybridization, and enzymatic activity. Fluorescence polarization assays are homogeneous in that they do not require a separation step such as centrifugation, filtration, chromatography, precipitation, or electrophoresis. These assays are done in real time, directly in solution and do not require an immobilized phase. Polarization values can be measured repeatedly and after the addition of reagents since measuring the polarization is rapid and does not destroy the sample. Generally, this technique can be used to measure polarization values of fluorophores from low picomolar to micromolar levels.

Activity Charts

Bioactivity

Activity Types for Assay ALA3888094

IC50

Total(314)

Compound Summary

Parent Molecular Weight	ALogP	Polar Surface Area
411.48	3.78	3.78
380.41	2.09	2.09
424.46	2.69	2.69
356.38	2.81	2.81
370.37	2.53	2.53
338.41	3.50	3.50
425.45	1.96	1.96
354.37	2.56	2.56
437.50	3.33	3.33
418.46	3.16	3.16
470.58	4.44	4.44
388.81	3.21	3.21
354.37	2.56	2.56
435.48	3.52	3.52
285.31	2.12	2.12
322.37	3.19	3.19
407.47	3.59	3.59
365.39	3.49	3.49
427.49	2.24	2.24
346.41	2.65	2.65
383.41	1.55	1.55
350.42	3.23	3.23
351.37	3.24	3.24
409.45	2.56	2.56
381.39	2.21	2.21
409.45	2.99	2.99
395.42	2.51	2.51
429.43	3.56	3.56
425.45	2.69	2.69
322.32	2.34	2.34
369.39	1.24	1.24
365.39	2.20	2.20
370.41	2.73	2.73
361.40	1.73	1.73
357.40	2.21	2.21
340.38	3.11	3.11
326.36	2.80	2.80
390.40	2.38	2.38
396.45	2.19	2.19
409.45	2.56	2.56
340.38	2.73	2.73
383.38	2.64	2.64
371.44	2.89	2.89
467.53	3.34	3.34
425.46	3.47	3.47
437.46	2.75	2.75
422.45	1.86	1.86
297.32	2.18	2.18
452.47	1.68	1.68
386.41	2.44	2.44
300.32	2.31	2.31
350.38	2.97	2.97
340.38	3.11	3.11
418.46	3.16	3.16
317.35	1.71	1.71
340.38	3.19	3.19
324.38	3.28	3.28
439.47	2.56	2.56
451.48	3.07	3.07
337.34	2.06	2.06
409.45	2.99	2.99
467.48	2.75	2.75
310.36	2.72	2.72
383.38	2.64	2.64
326.36	2.80	2.80
357.44	3.35	3.35
407.47	3.59	3.59
359.39	1.48	1.48
297.32	2.18	2.18
308.34	2.70	2.70
411.49	3.23	3.23
340.34	2.52	2.52
314.35	1.83	1.83
435.48	3.18	3.18
366.38	1.90	1.90
310.36	3.10	3.10
407.47	3.59	3.59
302.38	3.06	3.06
439.47	2.53	2.53
410.43	2.38	2.38
492.58	4.78	4.78
396.41	1.39	1.39
411.42	1.57	1.57
297.32	2.18	2.18
340.34	2.52	2.52
437.50	3.43	3.43
324.38	3.11	3.11
480.57	3.22	3.22
384.39	2.57	2.57
390.40	2.38	2.38
413.87	3.20	3.20
395.42	2.38	2.38
429.43	3.56	3.56
356.38	2.81	2.81
331.38	2.01	2.01
441.46	3.43	3.43
437.50	3.33	3.33
408.42	0.78	0.78
409.45	2.99	2.99
439.47	2.53	2.53
374.42	2.19	2.19
316.41	3.45	3.45
397.41	2.69	2.69
453.50	3.30	3.30
411.42	2.22	2.22
429.43	3.56	3.56
349.44	2.55	2.55
379.42	2.89	2.89
304.31	-0.28	-0.28
411.47	2.33	2.33
379.42	2.55	2.55
381.39	2.21	2.21
424.46	2.69	2.69
393.40	2.32	2.32
409.45	2.56	2.56
424.46	1.35	1.35
395.42	2.21	2.21
425.45	2.69	2.69
206.25	1.58	1.58
340.38	2.25	2.25
381.39	1.91	1.91
393.45	3.28	3.28
411.47	2.33	2.33
394.44	2.68	2.68
370.41	2.73	2.73
289.30	0.93	0.93
451.48	3.07	3.07
300.32	2.31	2.31
421.50	3.90	3.90
296.33	2.79	2.79
379.42	2.81	2.81
437.50	3.60	3.60
414.90	2.91	2.91
423.43	2.29	2.29
396.41	1.91	1.91
443.45	3.60	3.60
275.27	0.59	0.59
324.38	3.28	3.28
354.45	3.69	3.69
453.50	3.30	3.30
421.50	3.90	3.90
421.50	3.90	3.90
365.39	2.51	2.51
397.41	2.69	2.69
395.42	2.25	2.25
423.47	3.29	3.29
379.42	2.81	2.81
395.42	2.60	2.60
409.40	2.20	2.20
300.32	2.69	2.69
393.45	2.98	2.98
418.46	3.83	3.83
393.40	2.14	2.14
380.41	1.24	1.24
394.44	2.68	2.68
372.38	2.51	2.51
356.38	2.81	2.81
393.45	2.94	2.94
394.44	2.68	2.68
451.48	3.05	3.05
413.87	3.20	3.20
427.44	3.13	3.13
410.43	1.30	1.30
326.36	2.80	2.80
425.45	2.69	2.69
421.50	3.72	3.72
384.40	2.11	2.11
425.46	3.73	3.73
338.41	3.45	3.45
423.47	2.78	2.78
397.44	2.02	2.02
342.36	2.50	2.50
384.39	2.57	2.57
356.38	2.81	2.81
441.46	3.43	3.43
342.36	2.50	2.50
410.43	1.27	1.27
327.41	3.35	3.35
437.46	2.98	2.98
386.46	4.31	4.31
422.45	1.67	1.67
379.42	2.55	2.55
368.39	2.78	2.78
423.47	2.78	2.78
352.42	3.22	3.22
494.55	3.53	3.53
356.38	2.43	2.43
363.38	2.63	2.63
381.39	2.21	2.21
394.44	1.72	1.72
453.50	2.65	2.65
397.39	1.61	1.61
429.43	3.56	3.56
376.32	3.11	3.11
467.53	3.31	3.31
422.49	2.57	2.57
439.47	3.00	3.00
314.35	3.00	3.00
412.43	1.57	1.57
425.46	3.47	3.47
395.42	2.00	2.00
437.46	2.82	2.82
341.44	3.02	3.02
407.47	3.84	3.84
461.44	4.30	4.30
433.26	3.32	3.32
340.38	2.73	2.73
408.46	2.11	2.11
371.44	2.93	2.93
433.39	3.52	3.52
348.37	2.73	2.73
423.47	3.24	3.24
392.44	2.35	2.35
370.41	2.73	2.73
336.40	3.28	3.28
314.32	2.93	2.93
425.45	2.52	2.52
481.51	2.84	2.84
365.44	3.37	3.37
437.50	3.43	3.43
340.38	2.86	2.86
321.34	2.66	2.66
357.41	2.54	2.54
441.46	3.43	3.43
423.47	3.29	3.29
395.42	2.18	2.18
411.44	3.42	3.42
409.40	2.23	2.23
429.48	3.70	3.70
362.41	2.35	2.35
352.42	3.22	3.22
423.47	3.16	3.16
407.43	2.74	2.74
388.81	3.21	3.21
422.49	2.50	2.50
451.48	3.05	3.05
307.36	3.70	3.70
285.31	2.12	2.12
311.35	2.49	2.49
437.46	2.98	2.98
393.40	2.14	2.14
411.44	3.42	3.42
423.47	3.29	3.29
379.42	2.81	2.81
383.38	2.64	2.64
348.37	2.73	2.73
395.42	2.51	2.51
437.50	3.33	3.33
347.38	1.08	1.08
408.42	1.99	1.99
338.41	3.67	3.67
425.46	3.47	3.47
421.46	3.13	3.13
338.41	3.30	3.30
338.41	3.67	3.67
345.40	3.48	3.48
337.34	2.93	2.93
340.38	2.86	2.86
453.50	3.30	3.30
299.33	2.13	2.13
317.35	1.62	1.62
453.50	3.30	3.30
328.33	1.59	1.59
387.47	3.36	3.36
290.37	2.91	2.91
384.39	2.91	2.91
397.41	2.69	2.69
341.44	3.65	3.65
305.29	-0.05	-0.05
330.78	3.44	3.44
358.35	2.21	2.21
336.36	2.66	2.66
337.34	1.53	1.53
336.35	3.53	3.53
338.41	3.30	3.30
407.47	3.33	3.33
411.44	3.42	3.42
379.42	2.89	2.89
340.38	2.73	2.73
390.40	2.38	2.38
395.42	2.51	2.51
379.42	2.59	2.59
356.38	2.43	2.43
368.48	3.94	3.94
365.39	2.20	2.20
439.47	3.00	3.00
394.39	0.59	0.59
328.40	2.74	2.74
336.39	3.21	3.21
342.36	2.50	2.50
410.39	2.41	2.41
354.41	3.04	3.04
350.38	2.48	2.48
426.43	1.92	1.92
289.30	0.98	0.98
300.32	2.69	2.69
456.51	2.75	2.75
412.45	2.89	2.89
299.33	2.43	2.43
366.43	2.48	2.48
350.38	3.84	3.84
365.39	2.20	2.20
395.42	2.60	2.60
340.38	2.86	2.86
324.38	3.28	3.28
379.42	2.89	2.89
393.40	2.14	2.14