Basic Information
ID: ALA3888108
Type: Binding
Description: Beta-Lactamase Enzyme Inhibition Assay: For the measurement of β-lactamase inhibitory activity, 100 uM (final concentration) nitrocefin (Oxoid) was used as a substrate, and 2.5% DMSO, 10 ug/mL bovine serum derived albumin (Sigma-Aldrich) and 50 mM phosphate buffer at pH 7.0 were used as a reaction solution. To each well of a 96-well plate were added test compounds (compounds shown in Table 14) and AmpC (final concentration 0.5 nM), followed by pre-incubation at 30° C. for 10 minutes. Nitrocefin was added to each well to be mixed therein, followed by incubation at 30° C. for 20 minutes, and Multiskan Ascent (Thermo Fisher Scientific) was used to measure 492 nm wavelength, thereby measuring nitrocefin hydrolytic activity of AmpC, to determine enzyme inhibitory activity.
Activity Charts
Bioactivity
Activity Types for Assay ALA3888108
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 287.23 | -1.53 | -1.53 |
| 287.23 | -1.53 | -1.53 |
| 271.23 | -1.46 | -1.46 |
| 322.28 | -1.52 | -1.52 |