Basic Information
ID: ALA3888176
Type: Binding
Description: In Vitro Enzymatic BACE FRET Assay: The assay buffer used in this screen is 0.05 M acetate, pH 4.2, 10% DMSO final, 100 uM genapol (which is a nonionic detergent, below its Critical Micelle Concentration). The Beta Secretase enzyme (0.2 nM) is pre-incubated for one hour with inhibitors, typically in about 1 uL of DMSO according to a serial dilution, are added thereto. This assay is effectively started by the addition of FRET substrate (50 nM) and the combination is incubated for one hour. The FRET assay is terminated with by addition of Tris buffer, which raises the pH to neutrality, and the fluorescence is determined. The FRET substrate is a peptide with commercially available fluorophore and quencher, on opposite sides of the BACE cleavage site. Proteolytic cleavage of the FRET substrate releases quenching of fluorescence (excitation 488 nm and emission 425 nm).
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3888176
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 439.34 | 3.18 | 3.18 |
| 421.35 | 3.23 | 3.23 |
| 529.80 | 4.34 | 4.34 |
| 466.77 | 4.10 | 4.10 |
| 469.83 | 4.19 | 4.19 |
| 445.38 | 3.66 | 3.66 |
| 483.40 | 3.10 | 3.10 |
| 420.77 | 4.45 | 4.45 |
| 464.85 | 4.49 | 4.49 |
| 478.37 | 4.74 | 4.74 |
| 448.78 | 3.96 | 3.96 |
| 459.38 | 3.02 | 3.02 |
| 512.36 | 4.24 | 4.24 |
| 452.40 | 4.22 | 4.22 |
| 462.84 | 3.78 | 3.78 |
| 434.38 | 2.46 | 2.46 |
| 511.22 | 4.21 | 4.21 |
| 468.39 | 3.94 | 3.94 |
| 439.36 | 5.01 | 5.01 |
| 443.81 | 3.27 | 3.27 |
| 487.76 | 4.15 | 4.15 |
| 459.38 | 3.21 | 3.21 |
| 463.34 | 2.85 | 2.85 |
| 430.79 | 3.82 | 3.82 |
| 480.37 | 4.35 | 4.35 |
| 477.37 | 3.66 | 3.66 |
| 439.34 | 4.04 | 4.04 |
| 422.34 | 2.62 | 2.62 |
| 480.37 | 4.35 | 4.35 |
| 483.40 | 3.02 | 3.02 |
| 501.39 | 3.24 | 3.24 |
| 442.80 | 5.25 | 5.25 |
| 481.78 | 4.34 | 4.34 |
| 426.37 | 3.17 | 3.17 |
| 462.42 | 5.46 | 5.46 |
| 471.82 | 5.00 | 5.00 |
| 480.37 | 4.54 | 4.54 |
| 409.36 | 4.09 | 4.09 |
| 452.37 | 2.16 | 2.16 |
| 432.42 | 3.35 | 3.35 |
| 476.36 | 4.54 | 4.54 |
| 453.83 | 4.86 | 4.86 |
| 310.23 | 1.63 | 1.63 |
| 493.23 | 4.26 | 4.26 |
| 455.80 | 3.84 | 3.84 |
| 467.40 | 4.54 | 4.54 |
| 409.36 | 4.09 | 4.09 |
| 445.35 | 2.71 | 2.71 |
| 445.34 | 3.63 | 3.63 |
| 450.39 | 3.37 | 3.37 |
| 489.81 | 5.14 | 5.14 |
| 444.36 | 3.50 | 3.50 |
| 457.33 | 3.99 | 3.99 |
| 475.32 | 3.45 | 3.45 |
| 449.77 | 3.35 | 3.35 |
| 461.80 | 3.41 | 3.41 |
| 498.45 | 4.59 | 4.59 |
| 445.35 | 2.90 | 2.90 |
| 452.38 | 2.81 | 2.81 |
| 412.32 | 3.72 | 3.72 |
| 465.42 | 4.38 | 4.38 |
| 473.79 | 4.02 | 4.02 |
| 499.77 | 4.42 | 4.42 |
| 511.22 | 4.39 | 4.39 |
| 462.81 | 4.27 | 4.27 |
| 433.38 | 4.54 | 4.54 |
| 421.75 | 4.50 | 4.50 |
| 436.37 | 2.93 | 2.93 |
| 478.81 | 4.16 | 4.16 |
| 483.22 | 4.80 | 4.80 |
| 448.41 | 2.77 | 2.77 |
| 432.32 | 3.44 | 3.44 |
| 454.36 | 2.88 | 2.88 |
| 492.40 | 4.86 | 4.86 |
| 439.34 | 3.77 | 3.77 |
| 482.41 | 3.90 | 3.90 |
| 498.79 | 4.89 | 4.89 |
| 463.37 | 3.80 | 3.80 |
| 452.38 | 2.81 | 2.81 |
| 501.84 | 4.52 | 4.52 |
| 462.81 | 4.46 | 4.46 |
| 436.37 | 2.93 | 2.93 |
| 527.37 | 4.14 | 4.14 |
| 455.80 | 3.88 | 3.88 |
| 494.40 | 4.74 | 4.74 |
| 484.76 | 4.24 | 4.24 |
| 462.35 | 3.45 | 3.45 |
| 495.36 | 3.69 | 3.69 |
| 440.33 | 2.57 | 2.57 |
| 443.81 | 2.57 | 2.57 |
| 467.40 | 3.79 | 3.79 |
| 430.79 | 4.01 | 4.01 |
| 516.78 | 5.17 | 5.17 |
| 487.76 | 4.34 | 4.34 |
| 494.37 | 4.21 | 4.21 |
| 464.46 | 4.28 | 4.28 |
| 510.67 | 4.17 | 4.17 |
| 448.78 | 4.15 | 4.15 |
| 445.35 | 2.71 | 2.71 |
| 475.83 | 3.72 | 3.72 |
| 465.23 | 4.66 | 4.66 |
| 439.36 | 5.01 | 5.01 |
| 461.80 | 3.41 | 3.41 |
| 396.32 | 3.59 | 3.59 |
| 526.38 | 4.74 | 4.74 |
| 452.37 | 2.16 | 2.16 |
| 487.36 | 2.85 | 2.85 |
| 427.36 | 2.76 | 2.76 |
| 527.68 | 4.72 | 4.72 |
| 433.38 | 4.54 | 4.54 |
| 421.35 | 3.90 | 3.90 |
| 451.38 | 2.76 | 2.76 |
| 470.76 | 3.60 | 3.60 |
| 428.35 | 2.15 | 2.15 |
| 468.38 | 3.51 | 3.51 |
| 453.37 | 3.67 | 3.67 |
| 489.81 | 5.14 | 5.14 |
| 461.34 | 4.05 | 4.05 |
| 486.83 | 4.70 | 4.70 |
| 500.40 | 3.84 | 3.84 |
| 467.40 | 4.35 | 4.35 |
| 478.37 | 4.55 | 4.55 |
| 462.81 | 4.27 | 4.27 |
| 463.37 | 4.29 | 4.29 |
| 433.38 | 4.54 | 4.54 |
| 445.78 | 5.12 | 5.12 |
| 442.80 | 5.25 | 5.25 |
| 464.34 | 4.43 | 4.43 |
| 469.37 | 3.37 | 3.37 |
| 456.79 | 3.27 | 3.27 |
| 465.23 | 4.66 | 4.66 |
| 519.45 | 5.24 | 5.24 |
| 492.83 | 4.27 | 4.27 |
| 395.33 | 3.70 | 3.70 |
| 449.77 | 3.35 | 3.35 |
| 471.82 | 5.19 | 5.19 |
| 466.22 | 4.06 | 4.06 |
| 509.69 | 4.77 | 4.77 |
| 506.36 | 4.76 | 4.76 |
| 478.81 | 3.97 | 3.97 |
| 456.44 | 3.35 | 3.35 |
| 439.74 | 4.64 | 4.64 |
| 440.35 | 4.41 | 4.41 |
| 433.38 | 4.54 | 4.54 |
| 500.65 | 5.26 | 5.26 |
| 478.87 | 4.80 | 4.80 |
| 420.34 | 3.32 | 3.32 |
| 454.82 | 4.44 | 4.44 |
| 486.37 | 3.45 | 3.45 |
| 473.79 | 3.83 | 3.83 |
| 466.20 | 4.61 | 4.61 |
| 512.40 | 3.46 | 3.46 |
| 461.80 | 3.41 | 3.41 |
| 455.31 | 4.86 | 4.86 |
| 405.30 | 3.98 | 3.98 |
| 498.79 | 5.08 | 5.08 |
| 508.39 | 4.52 | 4.52 |
| 531.47 | 4.23 | 4.23 |
| 478.81 | 4.16 | 4.16 |
| 466.77 | 4.25 | 4.25 |
| 444.36 | 3.31 | 3.31 |
| 456.79 | 3.27 | 3.27 |
| 463.37 | 3.80 | 3.80 |
| 493.23 | 4.07 | 4.07 |
| 469.37 | 2.71 | 2.71 |
| 473.30 | 5.00 | 5.00 |
| 496.44 | 4.20 | 4.20 |
| 462.38 | 4.40 | 4.40 |
| 516.78 | 4.98 | 4.98 |
| 463.41 | 5.51 | 5.51 |
| 400.38 | 3.50 | 3.50 |
| 431.78 | 3.40 | 3.40 |
| 377.31 | 2.55 | 2.55 |
| 462.35 | 3.64 | 3.64 |
| 460.82 | 4.02 | 4.02 |
| 471.36 | 3.62 | 3.62 |
| 467.40 | 3.86 | 3.86 |
| 469.37 | 2.90 | 2.90 |
| 464.34 | 4.24 | 4.24 |
| 468.39 | 3.75 | 3.75 |
| 455.80 | 3.88 | 3.88 |
| 497.85 | 6.16 | 6.16 |
| 527.37 | 3.95 | 3.95 |
| 415.32 | 2.89 | 2.89 |
| 465.33 | 3.83 | 3.83 |
| 473.40 | 3.32 | 3.32 |
| 468.39 | 3.75 | 3.75 |
| 461.80 | 3.41 | 3.41 |
| 513.35 | 3.83 | 3.83 |
| 488.75 | 3.55 | 3.55 |
| 466.77 | 4.10 | 4.10 |
| 512.40 | 3.27 | 3.27 |
| 416.35 | 3.80 | 3.80 |
| 450.44 | 4.30 | 4.30 |
| 470.76 | 3.60 | 3.60 |
| 501.46 | 5.10 | 5.10 |
| 467.51 | ||
| 444.36 | 3.20 | 3.20 |
| 472.41 | 3.93 | 3.93 |
| 540.41 | 4.86 | 4.86 |
| 494.37 | 4.40 | 4.40 |
| 453.37 | 3.48 | 3.48 |
| 411.33 | 3.67 | 3.67 |
| 421.35 | 3.04 | 3.04 |
| 458.39 | 3.81 | 3.81 |
| 416.35 | 3.99 | 3.99 |
| 492.83 | 4.29 | 4.29 |
| 498.45 | 4.40 | 4.40 |
| 472.81 | 4.39 | 4.39 |
| 478.42 | 4.07 | 4.07 |
| 444.82 | 4.32 | 4.32 |
| 469.37 | 3.18 | 3.18 |
| 445.80 | 3.71 | 3.71 |
| 485.83 | 3.41 | 3.41 |
| 496.41 | 4.20 | 4.20 |
| 465.22 | 4.56 | 4.56 |
| 446.35 | 3.75 | 3.75 |
| 459.38 | 3.02 | 3.02 |
| 483.22 | 4.61 | 4.61 |
| 446.37 | 3.06 | 3.06 |
| 388.30 | 3.24 | 3.24 |
| 421.75 | 4.50 | 4.50 |
| 466.77 | 4.29 | 4.29 |
| 388.30 | 3.24 | 3.24 |
| 458.39 | 3.62 | 3.62 |
| 404.31 | 3.93 | 3.93 |
| 455.31 | 4.86 | 4.86 |
| 478.81 | 3.97 | 3.97 |
| 387.31 | 3.84 | 3.84 |
| 445.35 | 2.71 | 2.71 |