ID: ALA3888221
Type: Binding
Description: Inhibition Assay: 5 uM ATP: Test compounds dissolved and diluted in DMSO (2 μl) were added to a reaction mixture comprising 10 μl of 5× Reaction Buffer (40 mM MOPS pH 7.0, 5 mM EDTA), 10 μl of recombinant human PIM2 solution (4 ng PIM-2 dissolved in dilution buffer (20 mM MOPS pH 7.0; EDTA 1 mM; 5% Glycerol; 0.01% Brij 35; 0.1%; 0.1% 2-mercaptoethanol; 1 mg/ml BSA)) and 8 ul of water. Reactions were initiated by the addition of 10 ul of ATP Solution (49% (15 mM MgCl2; 5 uM ATP) 1% ([γ-33P]ATP: Stock 1 mCi/100 μl; 3000 Ci/mmol (Perkin Elmer)) and 10 ul of substrate peptide solution (RSRSSYPAGT (SEQ ID NO.: 6), dissolved in water at a concentration of 1 mM), Reactions were maintained for 10 min at 30° C. The reactions were quenched with 100 ul of 0.75% Phosphoric acid, then transferred to and filtered through a Phosphocellulose filter plate (Millipore, MSPH-N6B-50). After washing each well 4 times with 0.75% Phosphoric acid, scintillation fluid (20 uL) was added to each well and
Bioactivity
Activity Types for Assay ALA3888221
IC50
Parent Molecular Weight | ALogP | Polar Surface Area |
---|---|---|
413.83 | 1.71 | 1.71 |
391.39 | 0.89 | 0.89 |
415.52 | 2.38 | 2.38 |
465.54 | 2.69 | 2.69 |
404.43 | 2.47 | 2.47 |
547.50 | 1.73 | 1.73 |
378.37 | 2.29 | 2.29 |
402.41 | 2.35 | 2.35 |
506.59 | 1.99 | 1.99 |
505.48 | 2.89 | 2.89 |
398.43 | 0.39 | 0.39 |
397.44 | 0.39 | 0.39 |
403.45 | 2.22 | 2.22 |
444.37 | 3.05 | 3.05 |
407.46 | 3.30 | 3.30 |
433.48 | 1.56 | 1.56 |
492.42 | 2.40 | 2.40 |
479.52 | 2.06 | 2.06 |
358.36 | -0.33 | -0.33 |
425.45 | 0.33 | 0.33 |
396.37 | 0.18 | 0.18 |
439.48 | 0.60 | 0.60 |
354.37 | 0.84 | 0.84 |
432.44 | 0.95 | 0.95 |
522.64 | 2.53 | 2.53 |
344.40 | 1.69 | 1.69 |
364.32 | 0.56 | 0.56 |
362.39 | 2.01 | 2.01 |
534.65 | 2.82 | 2.82 |
521.60 | 2.85 | 2.85 |
446.48 | 0.60 | 0.60 |
460.38 | 2.52 | 2.52 |
477.55 | 2.84 | 2.84 |
449.50 | 2.10 | 2.10 |
492.57 | 1.69 | 1.69 |
493.59 | 2.82 | 2.82 |
374.43 | 1.42 | 1.42 |
540.61 | 3.24 | 3.24 |
506.59 | 1.99 | 1.99 |
475.51 | 1.45 | 1.45 |
478.54 | 1.39 | 1.39 |
450.46 | 1.51 | 1.51 |
390.40 | 2.16 | 2.16 |
400.40 | 1.60 | 1.60 |
442.87 | 2.15 | 2.15 |
383.42 | 0.69 | 0.69 |
536.62 | 1.66 | 1.66 |
411.47 | 0.63 | 0.63 |
500.54 | 2.54 | 2.54 |
442.36 | 2.00 | 2.00 |
384.40 | -0.16 | -0.16 |
384.40 | 0.14 | 0.14 |
427.83 | 3.02 | 3.02 |
480.55 | 1.50 | 1.50 |
463.57 | 3.24 | 3.24 |
386.42 | 0.31 | 0.31 |
354.37 | 0.48 | 0.48 |
507.58 | 2.46 | 2.46 |
376.38 | 1.86 | 1.86 |
412.36 | 1.90 | 1.90 |
331.36 | 0.60 | 0.60 |
368.40 | 1.23 | 1.23 |
478.58 | 1.96 | 1.96 |
439.86 | 2.89 | 2.89 |
455.48 | 1.91 | 1.91 |
493.59 | 2.82 | 2.82 |
400.85 | 2.87 | 2.87 |
518.55 | 2.12 | 2.12 |
491.46 | 2.50 | 2.50 |
461.51 | 2.22 | 2.22 |
437.89 | 3.52 | 3.52 |
372.45 | 2.70 | 2.70 |
372.39 | 0.06 | 0.06 |
426.89 | 4.02 | 4.02 |
480.96 | 3.20 | 3.20 |
372.36 | 0.43 | 0.43 |
354.37 | 0.70 | 0.70 |
438.47 | 1.55 | 1.55 |
397.32 | 0.36 | 0.36 |
409.84 | 2.88 | 2.88 |
404.43 | 1.61 | 1.61 |
451.80 | 3.28 | 3.28 |
370.37 | -0.55 | -0.55 |
397.44 | 0.56 | 0.56 |
421.42 | 0.42 | 0.42 |
344.34 | -0.72 | -0.72 |
521.56 | 0.11 | 0.11 |
460.89 | 3.31 | 3.31 |
386.44 | 1.46 | 1.46 |
493.55 | 2.07 | 2.07 |
367.41 | 1.69 | 1.69 |
440.85 | 2.28 | 2.28 |
472.55 | 1.90 | 1.90 |
361.37 | 1.55 | 1.55 |
408.44 | 2.42 | 2.42 |
424.44 | 2.00 | 2.00 |
478.54 | 1.39 | 1.39 |
426.44 | 0.93 | 0.93 |
434.46 | 2.06 | 2.06 |
405.42 | 1.25 | 1.25 |
394.37 | 1.76 | 1.76 |
405.42 | 1.08 | 1.08 |
427.85 | 2.10 | 2.10 |
393.38 | 2.37 | 2.37 |
386.42 | 0.31 | 0.31 |
409.43 | 1.31 | 1.31 |
445.36 | 2.44 | 2.44 |
449.54 | 2.81 | 2.81 |
372.39 | 0.33 | 0.33 |
340.35 | 0.45 | 0.45 |
520.62 | 2.43 | 2.43 |
465.54 | 2.04 | 2.04 |
501.47 | 2.62 | 2.62 |
506.59 | 2.04 | 2.04 |
376.38 | 1.62 | 1.62 |
458.48 | 1.53 | 1.53 |
437.46 | 0.96 | 0.96 |
520.62 | 2.38 | 2.38 |
474.55 | 0.76 | 0.76 |
493.55 | 1.81 | 1.81 |
478.54 | 1.30 | 1.30 |
413.51 | 1.77 | 1.77 |
467.51 | 1.32 | 1.32 |
444.38 | 2.64 | 2.64 |
376.38 | 1.86 | 1.86 |
385.39 | 2.02 | 2.02 |
397.44 | 0.02 | 0.02 |
551.05 | 4.15 | 4.15 |
486.52 | 2.86 | 2.86 |
397.44 | 0.39 | 0.39 |
404.43 | 2.05 | 2.05 |
476.32 | 1.71 | 1.71 |
477.55 | 2.69 | 2.69 |
506.59 | 2.03 | 2.03 |
465.54 | 2.74 | 2.74 |
467.51 | 1.32 | 1.32 |
412.45 | 0.84 | 0.84 |
412.36 | 1.90 | 1.90 |
460.86 | 2.29 | 2.29 |
411.47 | 0.78 | 0.78 |
427.53 | 2.52 | 2.52 |
454.49 | 1.56 | 1.56 |
482.98 | 3.31 | 3.31 |
474.53 | 1.37 | 1.37 |
461.93 | 4.52 | 4.52 |
434.46 | 1.91 | 1.91 |
461.53 | 2.48 | 2.48 |
406.47 | 2.75 | 2.75 |
459.51 | 1.98 | 1.98 |
524.97 | 2.36 | 2.36 |
358.34 | 0.04 | 0.04 |
453.48 | 1.06 | 1.06 |
556.60 | 2.67 | 2.67 |
506.59 | 2.03 | 2.03 |
463.52 | 2.35 | 2.35 |
492.61 | 2.35 | 2.35 |
368.40 | 1.09 | 1.09 |
369.39 | -0.58 | -0.58 |
423.86 | 3.13 | 3.13 |
492.52 | 0.78 | 0.78 |
491.48 | 1.76 | 1.76 |
413.49 | 0.88 | 0.88 |
433.47 | 3.01 | 3.01 |
363.36 | -0.72 | -0.72 |
416.44 | 2.72 | 2.72 |
476.95 | 4.60 | 4.60 |
537.58 | 1.21 | 1.21 |
443.39 | 3.25 | 3.25 |
413.44 | 0.32 | 0.32 |
503.55 | 1.88 | 1.88 |
423.46 | 1.57 | 1.57 |
379.36 | 1.68 | 1.68 |
423.86 | 3.27 | 3.27 |
544.02 | 3.78 | 3.78 |
459.39 | 3.13 | 3.13 |
467.51 | 1.59 | 1.59 |
418.46 | 2.43 | 2.43 |
439.86 | 2.89 | 2.89 |
405.42 | 2.24 | 2.24 |
522.64 | 2.53 | 2.53 |
495.57 | 2.69 | 2.69 |
386.39 | 0.82 | 0.82 |
510.94 | 2.95 | 2.95 |
481.51 | 2.40 | 2.40 |
553.02 | 4.12 | 4.12 |
369.77 | 1.89 | 1.89 |
467.49 | 2.01 | 2.01 |
342.36 | 0.70 | 0.70 |
428.82 | 2.42 | 2.42 |
441.45 | 2.41 | 2.41 |
406.47 | 2.78 | 2.78 |
399.41 | 1.95 | 1.95 |
520.62 | 2.38 | 2.38 |
487.52 | 2.15 | 2.15 |
418.41 | 1.94 | 1.94 |
437.49 | 1.91 | 1.91 |
410.83 | 2.28 | 2.28 |
449.50 | 2.10 | 2.10 |
404.43 | 2.20 | 2.20 |
520.62 | 2.43 | 2.43 |
382.31 | 0.85 | 0.85 |
460.89 | 3.31 | 3.31 |
409.84 | 2.88 | 2.88 |
412.36 | 1.90 | 1.90 |
520.62 | 2.43 | 2.43 |
477.83 | 3.90 | 3.90 |
451.51 | 2.35 | 2.35 |
391.42 | 2.08 | 2.08 |
495.54 | 2.79 | 2.79 |
520.98 | 3.12 | 3.12 |
506.59 | 2.04 | 2.04 |
467.51 | 1.55 | 1.55 |
507.58 | 2.46 | 2.46 |
424.85 | 2.52 | 2.52 |
658.12 | 4.74 | 4.74 |
508.61 | 2.28 | 2.28 |
372.39 | 0.06 | 0.06 |
473.47 | 2.21 | 2.21 |
437.89 | 3.66 | 3.66 |
343.35 | -0.23 | -0.23 |
411.37 | 2.51 | 2.51 |
382.43 | 0.65 | 0.65 |
410.42 | 1.91 | 1.91 |
508.61 | 2.24 | 2.24 |
438.88 | 2.08 | 2.08 |
492.61 | 2.35 | 2.35 |
481.54 | 1.98 | 1.98 |
466.50 | 2.28 | 2.28 |
532.58 | 2.46 | 2.46 |
397.44 | 0.02 | 0.02 |
548.67 | 3.21 | 3.21 |
361.37 | 1.55 | 1.55 |
380.43 | 2.52 | 2.52 |
440.90 | 2.28 | 2.28 |
550.69 | 3.45 | 3.45 |
412.48 | 2.99 | 2.99 |
431.46 | 1.79 | 1.79 |
493.55 | 1.81 | 1.81 |
478.82 | 3.29 | 3.29 |
481.54 | 1.98 | 1.98 |
453.48 | 1.52 | 1.52 |
370.37 | -0.28 | -0.28 |
314.31 | -0.08 | -0.08 |
368.40 | 0.73 | 0.73 |
418.44 | -0.52 | -0.52 |
494.99 | 3.59 | 3.59 |
534.65 | 2.82 | 2.82 |
520.62 | 2.43 | 2.43 |
492.57 | 1.69 | 1.69 |
299.29 | 0.07 | 0.07 |
300.28 | -0.54 | -0.54 |
500.54 | 2.54 | 2.54 |
404.43 | 2.18 | 2.18 |
285.35 | 2.47 | 2.47 |
460.89 | 3.31 | 3.31 |
440.47 | 2.64 | 2.64 |
328.34 | -0.06 | -0.06 |
397.44 | 0.56 | 0.56 |
413.51 | 1.77 | 1.77 |
482.93 | 2.05 | 2.05 |
397.44 | 0.56 | 0.56 |
509.01 | 3.84 | 3.84 |
396.41 | 2.83 | 2.83 |
404.43 | 2.05 | 2.05 |
492.95 | 3.61 | 3.61 |
513.53 | 3.08 | 3.08 |
390.41 | 1.49 | 1.49 |
427.83 | 3.02 | 3.02 |
340.35 | 0.09 | 0.09 |
506.57 | 1.25 | 1.25 |
467.53 | 1.48 | 1.48 |
520.62 | 2.43 | 2.43 |
586.06 | 3.83 | 3.83 |
494.58 | 1.89 | 1.89 |
479.47 | 2.79 | 2.79 |
403.40 | 1.25 | 1.25 |
489.54 | 1.86 | 1.86 |
358.36 | -0.33 | -0.33 |
440.92 | 4.37 | 4.37 |
478.54 | 1.39 | 1.39 |
369.39 | -0.35 | -0.35 |
496.96 | 2.18 | 2.18 |
453.48 | 1.85 | 1.85 |
459.39 | 2.34 | 2.34 |
492.57 | 1.60 | 1.60 |
463.52 | 2.45 | 2.45 |
358.36 | -0.33 | -0.33 |
510.99 | 2.56 | 2.56 |
516.61 | 3.40 | 3.40 |
463.52 | 2.49 | 2.49 |
467.51 | 1.91 | 1.91 |
398.43 | 0.60 | 0.60 |
391.39 | 0.89 | 0.89 |
410.42 | 1.91 | 1.91 |
368.40 | 0.87 | 0.87 |
499.50 | 2.69 | 2.69 |
495.57 | 1.96 | 1.96 |
390.40 | 1.64 | 1.64 |
509.59 | 2.76 | 2.76 |
476.42 | 2.52 | 2.52 |
449.50 | 2.06 | 2.06 |
424.85 | 2.14 | 2.14 |
515.56 | 2.24 | 2.24 |
394.37 | 1.76 | 1.76 |
428.37 | 3.17 | 3.17 |
411.85 | 3.13 | 3.13 |
459.51 | 2.22 | 2.22 |
356.39 | 0.95 | 0.95 |
492.52 | 1.58 | 1.58 |
471.48 | 3.03 | 3.03 |
433.48 | 1.56 | 1.56 |
562.66 | 2.15 | 2.15 |
405.42 | 0.93 | 0.93 |
537.02 | 3.76 | 3.76 |
427.83 | 3.02 | 3.02 |
369.39 | 0.30 | 0.30 |
462.54 | 2.35 | 2.35 |
383.42 | 0.04 | 0.04 |
523.99 | 2.86 | 2.86 |
433.43 | 0.59 | 0.59 |
396.33 | 1.24 | 1.24 |
558.00 | 3.10 | 3.10 |
506.59 | 1.99 | 1.99 |
494.58 | 1.89 | 1.89 |
477.55 | 2.88 | 2.88 |
493.55 | 2.12 | 2.12 |
452.78 | 2.68 | 2.68 |
396.46 | 0.99 | 0.99 |
479.52 | 1.68 | 1.68 |
493.55 | 1.81 | 1.81 |
453.53 | 1.95 | 1.95 |
446.47 | 2.43 | 2.43 |
489.88 | 3.75 | 3.75 |
441.88 | 2.75 | 2.75 |
396.46 | 1.17 | 1.17 |
506.59 | 2.04 | 2.04 |
437.49 | 1.91 | 1.91 |
403.39 | 1.72 | 1.72 |
481.54 | 2.30 | 2.30 |
430.47 | 2.94 | 2.94 |
452.91 | 2.47 | 2.47 |
385.39 | -0.46 | -0.46 |
411.81 | 2.32 | 2.32 |
390.41 | 1.87 | 1.87 |
449.54 | 2.81 | 2.81 |
505.54 | 1.33 | 1.33 |
398.43 | 0.59 | 0.59 |
367.37 | 0.83 | 0.83 |
506.59 | 2.04 | 2.04 |
477.93 | 3.54 | 3.54 |
384.40 | 0.20 | 0.20 |
389.42 | 1.61 | 1.61 |
372.45 | 2.47 | 2.47 |
465.54 | 2.60 | 2.60 |
353.39 | 1.31 | 1.31 |
545.99 | 5.01 | 5.01 |
481.94 | 3.27 | 3.27 |
438.49 | 1.34 | 1.34 |
404.38 | 1.11 | 1.11 |
427.47 | 0.01 | 0.01 |
438.42 | 1.16 | 1.16 |
466.52 | 3.17 | 3.17 |
453.48 | 0.93 | 0.93 |
478.54 | 1.30 | 1.30 |
367.41 | 1.84 | 1.84 |
495.57 | 2.37 | 2.37 |
520.58 | 1.52 | 1.52 |
328.34 | 0.31 | 0.31 |
406.41 | 1.63 | 1.63 |
385.41 | 0.63 | 0.63 |
445.38 | 1.63 | 1.63 |
432.44 | 0.95 | 0.95 |
367.41 | 1.69 | 1.69 |
493.55 | 2.12 | 2.12 |
384.40 | -0.16 | -0.16 |
437.89 | 3.52 | 3.52 |
370.37 | -0.55 | -0.55 |
379.38 | 0.88 | 0.88 |
371.38 | 1.03 | 1.03 |
478.54 | 1.39 | 1.39 |
545.50 | 3.28 | 3.28 |
404.43 | 2.05 | 2.05 |
413.44 | 0.32 | 0.32 |
397.44 | 0.56 | 0.56 |
372.39 | 0.06 | 0.06 |
368.40 | 1.09 | 1.09 |
481.51 | 2.40 | 2.40 |
460.52 | 0.57 | 0.57 |
372.36 | 0.43 | 0.43 |
420.43 | 1.02 | 1.02 |