Basic Information
ID: ALA3888305
Type: Binding
Description: GTPγS Binding Assay: Test method: wetting membranes by saponin solution, and preparing [35S]-GTPγS in binding solution (4×). The test compound was prepared as 4× of the final concentration which was then performed serial 3× dilution in 100% DMSO. Membranes with high-expressed S1P1 were collected in 5 ml binding buffer, and 50 μL was added to 96-well (containing 5 μg membrane protein with high-expressed S1P1). 25 μL, test compound was added in each well. The compound was incubated with the membranes for 30 min at room temperature, then 25 μL [35S]-GTPγS was added, and the experiment was initiated. The 96-well detection plate was incubated for 1 hour at room temperature, filtered by filtermat B, and washed with washing solution for three times. After the plate was dried for 1 h at 50° C., 8 ml scintillator solution was added and counted using scintillation counter.
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3888305
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 365.43 | 2.90 | 2.90 |
| 447.46 | 4.39 | 4.39 |
| 392.50 | 3.21 | 3.21 |
| 409.49 | 2.92 | 2.92 |
| 393.49 | 3.68 | 3.68 |
| 393.49 | 3.68 | 3.68 |
| 379.46 | 3.37 | 3.37 |
| 471.58 | 3.27 | 3.27 |
| 367.37 | 1.89 | 1.89 |
| 421.54 | 4.28 | 4.28 |
| 392.50 | 3.87 | 3.87 |
| 395.46 | 2.61 | 2.61 |
| 483.61 | 5.51 | 5.51 |
| 409.49 | 3.38 | 3.38 |
| 393.49 | 3.68 | 3.68 |
| 435.52 | 3.86 | 3.86 |
| 421.54 | 4.33 | 4.33 |
| 380.45 | 2.76 | 2.76 |
| 463.46 | 3.63 | 3.63 |