Basic Information
ID: ALA3888334
Type: Binding
Description: Cellular Assay: Inhibition of AKT was measured by Western blots using specific antibodies against phospho-Ser473-AKT in HT-29 lung cancer cells. Inhibition of the phosphorylation of AKT and its downstream targets was measured by Western blotting using rabbit polyclonal antibodies to phospho-Ser473-AKT, phospho-Thr308-AKT, total-AKT, phospho-Ser9-GSK3β, phospho-Ser21-GSK3β, phospho-Ser241-PDK1 and phospho-Thr389p70S6-kinase (New England Biolabs/Cell Signaling Technology Inc.) using β-Actin as a loading control as described in Mol Cancer Ther 7:2621 (2008). Bands corresponding to phospho-Ser473-AKT and total AKT were quantified using Eagle Eye software (BioRad) and Kodak X-Omat™ Blue XB (NEN™, Life Science Products). Cell growth inhibition was determined using a microcytotoxicity assay and apoptosis was measured as described in Mol Cancer Ther 7:2621 (2008).
Format: BAO_0000219
Cell type: Cancer cell lines
Target: Serine/threonine-protein kinase AKT(ALA4282)
Document: ALA3886664
BindingDB Source: 7909
Activity Charts
Bioactivity
Activity Types for Assay ALA3888334
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 256.31 | 0.92 | 0.92 |
| 298.35 | 1.30 | 1.30 |
| 416.48 | 0.96 | 0.96 |
| 497.92 | 3.44 | 3.44 |
| 410.57 | 4.42 | 4.42 |
| 409.62 | 5.80 | 5.80 |
| 371.43 | 0.53 | 0.53 |
| 469.50 | 1.70 | 1.70 |
| 517.98 | 3.81 | 3.81 |