Basic Information
ID: ALA3888335
Type: Binding
Description: Cellular Assay: Inhibition of AKT was measured by Western blotting using specific antibodies against phospho-Ser473-AKT in MiaPaCa-2 cells.Inhibition of AKT was measured by Western blots using specific antibodies against phospho-Ser473-AKT in HT-29 lung cancer cells. Inhibition of the phosphorylation of AKT and its downstream targets was measured by Western blotting using rabbit polyclonal antibodies to phospho-Ser473-AKT, phospho-Thr308-AKT, total-AKT, phospho-Ser9-GSK3β, phospho-Ser21-GSK3β, phospho-Ser241-PDK1 and phospho-Thr389p70S6-kinase (New England Biolabs/Cell Signaling Technology Inc.) using β-Actin as a loading control as described in Mol Cancer Ther 7:2621 (2008). Bands corresponding to phospho-Ser473-AKT and total AKT were quantified using Eagle Eye software (BioRad) and Kodak X-Omat™ Blue XB (NEN™, Life Science Products). Cell growth inhibition was determined using a microcytotoxicity assay and apoptosis was measured as described in Mol Cancer Ther 7:2621 (2008).
Format: BAO_0000219
Cell type: Cancer cell lines
Target: Serine/threonine-protein kinase AKT(ALA4282)
Document: ALA3886664
BindingDB Source: 7909
Activity Charts
Bioactivity
Activity Types for Assay ALA3888335
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 256.31 | 0.92 | 0.92 |
| 460.56 | 3.60 | 3.60 |
| 352.37 | 1.25 | 1.25 |
| 404.40 | 2.14 | 2.14 |
| 360.37 | 3.89 | 3.89 |
| 490.57 | 0.37 | 0.37 |
| 285.30 | 3.04 | 3.04 |
| 376.39 | 2.63 | 2.63 |