Basic Information
ID: ALA3888352
Type: Binding
Description: Enzyme Assay: P70S6K inhibitor compounds were diluted and plated in 96 well plates. A reaction mixture including the following components was then added to the compound plate to initiate the enzyme reaction; P70S6K (3 nM, T412E mutant, Millipore) was mixed with 24 μM ATP in an assay buffer containing 100 mM Hepes (pH 7.5), 5 mM MgCl2, 1 mM DTT, 0.015% Brij and 1 μM of the substrate peptide FITC-AHA-AKRRRLSSLRA-OH (derived from the S6 ribosomal protein sequence, FITC=fluorescein isothiocyanate, AHA=6-aminohexanoic acid). The reaction was incubated for 90 min at 25° C., before the addition of 10 mM EDTA to stop the reaction.
Format: BAO_0000357
Organism: Homo sapiens
Target: Ribosomal protein S6 kinase 1(ALA4501)
Document: ALA3886674
BindingDB Source: 7932
Activity Charts
Bioactivity
Activity Types for Assay ALA3888352
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 463.40 | 3.31 | 3.31 |
| 448.33 | 4.20 | 4.20 |
| 392.48 | 2.69 | 2.69 |
| 452.54 | 2.17 | 2.17 |
| 432.45 | 2.29 | 2.29 |
| 503.41 | 3.84 | 3.84 |
| 546.64 | 3.48 | 3.48 |
| 488.53 | 4.31 | 4.31 |
| 443.53 | 3.51 | 3.51 |
| 490.58 | 3.21 | 3.21 |
| 462.56 | 1.80 | 1.80 |
| 472.52 | 3.48 | 3.48 |
| 514.57 | 4.84 | 4.84 |
| 384.38 | 2.04 | 2.04 |
| 392.43 | 2.54 | 2.54 |
| 473.34 | 3.27 | 3.27 |
| 429.53 | 2.28 | 2.28 |
| 460.48 | 3.71 | 3.71 |
| 431.47 | 3.43 | 3.43 |
| 528.60 | 5.23 | 5.23 |
| 445.49 | 3.44 | 3.44 |
| 404.92 | 2.81 | 2.81 |
| 446.48 | 2.30 | 2.30 |
| 428.34 | 2.47 | 2.47 |
| 420.48 | 3.15 | 3.15 |
| 459.49 | 4.84 | 4.84 |
| 490.51 | 3.18 | 3.18 |
| 432.45 | 3.69 | 3.69 |
| 499.38 | 3.80 | 3.80 |
| 430.45 | 5.34 | 5.34 |
| 431.44 | 4.21 | 4.21 |
| 431.47 | 2.89 | 2.89 |
| 433.44 | 2.55 | 2.55 |
| 478.59 | 4.35 | 4.35 |
| 528.58 | 3.96 | 3.96 |
| 449.37 | 2.92 | 2.92 |
| 450.54 | 3.57 | 3.57 |
| 434.51 | 3.38 | 3.38 |
| 545.59 | 3.42 | 3.42 |
| 464.41 | 2.84 | 2.84 |
| 456.54 | 6.13 | 6.13 |
| 420.48 | 3.04 | 3.04 |
| 444.30 | 3.80 | 3.80 |
| 400.84 | 2.55 | 2.55 |
| 488.53 | 4.31 | 4.31 |
| 429.53 | 1.89 | 1.89 |
| 446.48 | 2.30 | 2.30 |
| 391.40 | 1.77 | 1.77 |
| 432.49 | 3.47 | 3.47 |
| 370.48 | 2.16 | 2.16 |
| 486.55 | 3.15 | 3.15 |
| 431.47 | 3.43 | 3.43 |
| 396.42 | 1.91 | 1.91 |
| 410.44 | 2.30 | 2.30 |
| 459.49 | 4.84 | 4.84 |
| 432.45 | 2.29 | 2.29 |
| 528.62 | 4.40 | 4.40 |
| 463.40 | 3.31 | 3.31 |
| 415.26 | 4.30 | 4.30 |
| 528.60 | 5.23 | 5.23 |
| 446.52 | 3.86 | 3.86 |
| 500.54 | 4.45 | 4.45 |
| 396.90 | 4.98 | 4.98 |
| 384.50 | 2.55 | 2.55 |
| 514.57 | 4.84 | 4.84 |
| 384.50 | 2.55 | 2.55 |
| 453.49 | 2.05 | 2.05 |
| 382.39 | 1.61 | 1.61 |
| 464.56 | 3.96 | 3.96 |
| 445.29 | 2.66 | 2.66 |
| 429.53 | 1.89 | 1.89 |
| 514.55 | 3.87 | 3.87 |
| 490.58 | 3.22 | 3.22 |
| 482.61 | 1.42 | 1.42 |
| 480.56 | 3.58 | 3.58 |
| 434.51 | 3.43 | 3.43 |
| 427.91 | 4.11 | 4.11 |
| 513.41 | 4.19 | 4.19 |
| 422.51 | 2.16 | 2.16 |
| 377.29 | 1.64 | 1.64 |
| 478.59 | 4.35 | 4.35 |
| 454.53 | 2.60 | 2.60 |
| 446.48 | 2.30 | 2.30 |
| 460.51 | 2.78 | 2.78 |
| 381.71 | 3.93 | 3.93 |
| 460.48 | 3.71 | 3.71 |
| 513.41 | 4.19 | 4.19 |
| 482.99 | 3.75 | 3.75 |
| 464.56 | 3.96 | 3.96 |
| 422.51 | 2.16 | 2.16 |
| 464.56 | 3.96 | 3.96 |
| 432.45 | 2.29 | 2.29 |
| 460.48 | 3.71 | 3.71 |
| 488.53 | 4.31 | 4.31 |
| 462.56 | 1.80 | 1.80 |
| 429.53 | 1.89 | 1.89 |
| 431.47 | 3.43 | 3.43 |
| 459.49 | 4.84 | 4.84 |
| 490.58 | 3.21 | 3.21 |
| 446.48 | 2.30 | 2.30 |