Basic Information
ID: ALA3888488
Type: Binding
Description: In Vitro Assay: HEK 293 cells stably transfected with either Nav 1.7 or with Nav 1.5 were recorded in population patch-clamp mode with the IonWorks® Quattro automated electrophysiology system in accordance with the manufacturer's specifications (Molecular Devices, LLC, Sunnyvale, Calif.). Sodium channel currents were measured in response to a train of depolarizations that induced successively greater inactivation. Cells were held at −110 mV for three seconds (Nav 1.7) or half a second (Nav 1.5) from a holding voltage of −15 mV, then put through a series of 26 pulses of 150 msec duration to −20 mV at a frequency of 5 Hz. Cells were then left unclamped for a period of 3 to 8 minutes while a single concentration of test compound was added. Cells were then reclamped and put through the same voltage protocol. Current at the end of the 26th pulse to −20 mV was subtracted from the peak current evoked by the 26th pulse to −20 mV to correct for leak current.
Cell type: Sf9
Activity Charts
Bioactivity
Activity Types for Assay ALA3888488
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 476.89 | 4.54 | 4.54 |
| 459.83 | 4.68 | 4.68 |
| 492.56 | 5.15 | 5.15 |
| 453.45 | 3.80 | 3.80 |
| 438.92 | 3.53 | 3.53 |
| 520.35 | 5.26 | 5.26 |
| 475.91 | 4.57 | 4.57 |
| 471.48 | 3.99 | 3.99 |
| 515.54 | 3.39 | 3.39 |
| 462.47 | 3.25 | 3.25 |
| 421.48 | 3.62 | 3.62 |
| 529.97 | 4.45 | 4.45 |
| 505.31 | 5.13 | 5.13 |
| 416.46 | 3.49 | 3.49 |
| 480.49 | 4.75 | 4.75 |
| 435.49 | 2.28 | 2.28 |
| 472.47 | 3.90 | 3.90 |
| 495.59 | 4.79 | 4.79 |
| 439.46 | 4.08 | 4.08 |
| 425.89 | 4.27 | 4.27 |
| 539.61 | 4.25 | 4.25 |
| 404.47 | 2.88 | 2.88 |
| 475.90 | 5.15 | 5.15 |
| 471.48 | 4.42 | 4.42 |
| 424.51 | 4.02 | 4.02 |
| 508.51 | 4.28 | 4.28 |
| 416.46 | 3.49 | 3.49 |
| 442.52 | 3.74 | 3.74 |
| 426.52 | 3.91 | 3.91 |
| 480.49 | 4.75 | 4.75 |
| 402.50 | 3.49 | 3.49 |
| 466.47 | 4.36 | 4.36 |
| 468.56 | 4.31 | 4.31 |
| 439.46 | 4.08 | 4.08 |
| 500.91 | 5.02 | 5.02 |
| 412.50 | 3.65 | 3.65 |
| 472.47 | 3.90 | 3.90 |
| 518.54 | 5.56 | 5.56 |
| 482.47 | 4.24 | 4.24 |
| 432.91 | 4.00 | 4.00 |
| 494.52 | 5.14 | 5.14 |
| 417.51 | 3.87 | 3.87 |
| 453.55 | 3.87 | 3.87 |
| 494.52 | 5.14 | 5.14 |
| 432.91 | 4.00 | 4.00 |
| 387.49 | 3.78 | 3.78 |
| 412.50 | 3.65 | 3.65 |
| 515.31 | 4.59 | 4.59 |
| 472.88 | 4.42 | 4.42 |
| 430.54 | 4.08 | 4.08 |
| 406.46 | 3.32 | 3.32 |
| 442.44 | 3.89 | 3.89 |
| 537.59 | 4.61 | 4.61 |
| 448.53 | 2.89 | 2.89 |
| 429.57 | 4.91 | 4.91 |
| 480.49 | 4.75 | 4.75 |
| 466.47 | 4.36 | 4.36 |
| 441.46 | 4.49 | 4.49 |
| 497.57 | 5.29 | 5.29 |
| 492.56 | 5.15 | 5.15 |
| 415.54 | 4.60 | 4.60 |
| 459.83 | 4.68 | 4.68 |
| 484.46 | 4.50 | 4.50 |
| 450.40 | 3.90 | 3.90 |
| 522.53 | 4.29 | 4.29 |
| 452.95 | 3.92 | 3.92 |
| 409.44 | 3.75 | 3.75 |
| 446.49 | 3.49 | 3.49 |
| 471.48 | 4.68 | 4.68 |
| 433.51 | 3.49 | 3.49 |
| 523.56 | 4.26 | 4.26 |
| 421.48 | 3.62 | 3.62 |
| 442.35 | 4.78 | 4.78 |
| 412.50 | 3.65 | 3.65 |
| 416.46 | 3.49 | 3.49 |
| 475.90 | 5.15 | 5.15 |
| 485.46 | 4.03 | 4.03 |
| 492.56 | 5.15 | 5.15 |
| 424.51 | 4.02 | 4.02 |
| 402.50 | 3.49 | 3.49 |
| 471.48 | 4.50 | 4.50 |
| 522.57 | 4.87 | 4.87 |
| 536.60 | 5.21 | 5.21 |
| 461.43 | 3.70 | 3.70 |
| 421.93 | 4.44 | 4.44 |
| 405.48 | 3.92 | 3.92 |
| 524.55 | 4.69 | 4.69 |
| 480.49 | 4.75 | 4.75 |
| 522.57 | 4.87 | 4.87 |
| 531.56 | 4.55 | 4.55 |
| 408.89 | 3.52 | 3.52 |
| 470.86 | 4.48 | 4.48 |
| 430.54 | 4.08 | 4.08 |
| 442.52 | 3.74 | 3.74 |
| 441.53 | 3.99 | 3.99 |
| 403.49 | 3.48 | 3.48 |
| 374.45 | 2.87 | 2.87 |
| 501.51 | 3.52 | 3.52 |
| 517.94 | 4.52 | 4.52 |
| 428.50 | 3.35 | 3.35 |
| 442.52 | 3.22 | 3.22 |
| 428.50 | 3.35 | 3.35 |
| 405.48 | 3.92 | 3.92 |
| 517.53 | 4.20 | 4.20 |
| 471.85 | 3.88 | 3.88 |
| 428.50 | 3.35 | 3.35 |
| 421.93 | 4.44 | 4.44 |
| 489.47 | 4.71 | 4.71 |
| 438.53 | 4.33 | 4.33 |
| 476.89 | 4.54 | 4.54 |
| 407.90 | 4.13 | 4.13 |
| 520.52 | 4.35 | 4.35 |
| 441.46 | 4.49 | 4.49 |
| 450.55 | 4.54 | 4.54 |
| 432.91 | 4.00 | 4.00 |
| 398.47 | 3.35 | 3.35 |
| 404.47 | 2.88 | 2.88 |
| 412.50 | 3.65 | 3.65 |
| 404.47 | 2.88 | 2.88 |
| 442.44 | 3.89 | 3.89 |
| 517.53 | 4.20 | 4.20 |
| 440.55 | 4.47 | 4.47 |
| 444.58 | 4.16 | 4.16 |
| 409.44 | 3.75 | 3.75 |
| 519.53 | 4.95 | 4.95 |
| 509.51 | 3.89 | 3.89 |
| 421.93 | 4.44 | 4.44 |
| 418.50 | 3.19 | 3.19 |
| 515.54 | 3.39 | 3.39 |
| 443.34 | 4.18 | 4.18 |
| 424.51 | 4.02 | 4.02 |
| 416.46 | 3.49 | 3.49 |
| 421.93 | 4.44 | 4.44 |
| 398.47 | 3.35 | 3.35 |
| 518.54 | 5.63 | 5.63 |
| 623.68 | 5.91 | 5.91 |
| 485.51 | 4.03 | 4.03 |
| 457.58 | 4.37 | 4.37 |
| 505.93 | 4.00 | 4.00 |
| 473.91 | 4.73 | 4.73 |
| 519.53 | 4.95 | 4.95 |
| 457.46 | 4.37 | 4.37 |
| 484.46 | 4.50 | 4.50 |
| 373.46 | 3.47 | 3.47 |
| 481.98 | 3.02 | 3.02 |
| 482.47 | 4.24 | 4.24 |
| 433.51 | 3.49 | 3.49 |
| 484.46 | 4.50 | 4.50 |
| 514.32 | 5.19 | 5.19 |
| 413.48 | 3.05 | 3.05 |
| 418.50 | 3.27 | 3.27 |
| 466.47 | 4.36 | 4.36 |
| 519.53 | 4.95 | 4.95 |
| 489.93 | 4.60 | 4.60 |
| 467.45 | 3.76 | 3.76 |
| 402.50 | 3.49 | 3.49 |
| 405.48 | 3.92 | 3.92 |
| 407.90 | 4.13 | 4.13 |
| 450.40 | 3.90 | 3.90 |
| 416.46 | 3.49 | 3.49 |
| 405.48 | 3.92 | 3.92 |
| 387.49 | 3.34 | 3.34 |
| 421.93 | 4.44 | 4.44 |
| 430.54 | 4.08 | 4.08 |
| 432.91 | 4.00 | 4.00 |
| 399.46 | 2.74 | 2.74 |
| 451.96 | 4.52 | 4.52 |
| 534.55 | 4.53 | 4.53 |
| 516.55 | 4.81 | 4.81 |
| 490.92 | 4.85 | 4.85 |
| 421.93 | 4.44 | 4.44 |
| 520.35 | 5.26 | 5.26 |
| 475.90 | 5.15 | 5.15 |
| 407.90 | 4.13 | 4.13 |
| 521.34 | 4.65 | 4.65 |
| 428.50 | 3.35 | 3.35 |
| 404.47 | 2.88 | 2.88 |
| 493.37 | 5.18 | 5.18 |
| 427.51 | 3.22 | 3.22 |
| 487.86 | 5.22 | 5.22 |
| 517.53 | 4.20 | 4.20 |
| 403.49 | 3.48 | 3.48 |
| 459.83 | 4.68 | 4.68 |
| 442.35 | 4.78 | 4.78 |
| 517.53 | 4.20 | 4.20 |
| 470.86 | 4.48 | 4.48 |
| 409.44 | 3.75 | 3.75 |
| 434.50 | 2.89 | 2.89 |
| 425.89 | 4.27 | 4.27 |
| 475.90 | 5.15 | 5.15 |
| 424.51 | 4.02 | 4.02 |
| 399.46 | 2.74 | 2.74 |
| 412.50 | 3.65 | 3.65 |
| 425.50 | 3.42 | 3.42 |
| 458.44 | 3.77 | 3.77 |
| 437.93 | 4.14 | 4.14 |
| 454.48 | 3.78 | 3.78 |
| 442.35 | 4.78 | 4.78 |
| 418.50 | 3.19 | 3.19 |
| 416.46 | 3.49 | 3.49 |
| 405.48 | 3.92 | 3.92 |
| 433.51 | 3.49 | 3.49 |
| 470.86 | 4.48 | 4.48 |
| 453.45 | 3.80 | 3.80 |
| 457.58 | 2.88 | 2.88 |
| 410.43 | 3.15 | 3.15 |
| 412.50 | 3.54 | 3.54 |
| 471.48 | 4.50 | 4.50 |
| 521.55 | 4.89 | 4.89 |
| 425.50 | 3.42 | 3.42 |
| 489.02 | 4.50 | 4.50 |
| 439.52 | 3.66 | 3.66 |
| 439.52 | 3.66 | 3.66 |
| 451.96 | 4.52 | 4.52 |
| 480.49 | 4.75 | 4.75 |
| 515.54 | 3.39 | 3.39 |
| 442.52 | 3.74 | 3.74 |
| 480.49 | 4.75 | 4.75 |