Basic Information
ID: ALA3888503
Type: Binding
Description: Fluorescence Polarization Assay (FPA): The assays were performed in black flat-bottom 384-well plates. The final assay volume was 50 μl prepared from additions of Gst-Bcl-2 (Bcl-2: GENBANK® Accession No. P10415), fluoresceinated 18-mer BIM peptide (NH2-YYANFEDGIRRLEQAIWI-[FAM]) (SEQ ID NO: 1), and test compounds in assay buffer consisting of 20 mM Sodium Phosphate, 1 mM EDTA, 50 mM NaCl, and 0.05% Pluronic Acid. The reaction was incubated at room temperature for 60 minutes and fluorescence polarization of the reaction was detected on the LJL Plate Reader. Inhibition data were calculated from mP values generated by the no protein control reactions for 100% inhibition and vehicle-only reactions for 0% inhibition. The final concentrations of reagents in the assay were 4.9 nM BCL-2, 5 nM fluoresceinated 18-mer BIM peptide and 1% DMSO.
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3888503
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 832.98 | 9.32 | 9.32 |
| 790.94 | 8.64 | 8.64 |
| 825.39 | 9.29 | 9.29 |
| 825.39 | 9.29 | 9.29 |
| 742.90 | 6.38 | 6.38 |
| 728.87 | 6.21 | 6.21 |
| 859.83 | 9.94 | 9.94 |
| 745.73 | 6.87 | 6.87 |
| 746.72 | 6.91 | 6.91 |
| 740.33 | 7.35 | 7.35 |
| 869.48 | 7.06 | 7.06 |
| 866.22 | 7.95 | 7.95 |
| 866.22 | 7.95 | 7.95 |
| 759.37 | 6.58 | 6.58 |
| 890.33 | 9.06 | 9.06 |
| 770.35 | 6.71 | 6.71 |
| 842.46 | 7.77 | 7.77 |
| 841.48 | 7.29 | 7.29 |
| 769.37 | 6.67 | 6.67 |
| 742.30 | 7.14 | 7.14 |
| 881.50 | 5.30 | 5.30 |
| 762.93 | 5.81 | 5.81 |
| 866.48 | 6.73 | 6.73 |
| 984.20 | 11.49 | 11.49 |
| 853.53 | 8.57 | 8.57 |
| 774.77 | 8.00 | 8.00 |
| 809.22 | 8.65 | 8.65 |
| 888.32 | 9.50 | 9.50 |
| 835.04 | 5.76 | 5.76 |
| 784.34 | 6.80 | 6.80 |
| 802.70 | 7.66 | 7.66 |
| 860.82 | 6.53 | 6.53 |
| 784.34 | 7.05 | 7.05 |
| 939.75 | 11.38 | 11.38 |
| 774.77 | 8.00 | 8.00 |
| 803.81 | 7.33 | 7.33 |
| 862.29 | 8.18 | 8.18 |
| 907.70 | 9.07 | 9.07 |
| 844.43 | 6.01 | 6.01 |
| 847.65 | 10.03 | 10.03 |
| 866.28 | 7.29 | 7.29 |
| 705.88 | 6.60 | 6.60 |
| 846.45 | 8.93 | 8.93 |
| 856.49 | 6.87 | 6.87 |
| 705.88 | 6.69 | 6.69 |
| 1002.38 | ||
| 811.41 | 7.05 | 7.05 |
| 777.00 | 7.01 | 7.01 |
| 765.34 | 7.22 | 7.22 |
| 1031.51 | ||
| 867.42 | 9.97 | 9.97 |
| 827.63 | 7.71 | 7.71 |
| 841.47 | 7.66 | 7.66 |
| 774.77 | 8.00 | 8.00 |
| 714.29 | 6.29 | 6.29 |
| 868.29 | 6.70 | 6.70 |
| 867.51 | 8.21 | 8.21 |
| 918.34 | 8.86 | 8.86 |
| 818.35 | 7.54 | 7.54 |
| 920.36 | 8.42 | 8.42 |
| 714.96 | ||
| 870.86 | 10.07 | 10.07 |
| 857.26 | 9.10 | 9.10 |
| 969.13 | 9.66 | 9.66 |
| 883.25 | 7.77 | 7.77 |
| 867.54 | 8.35 | 8.35 |
| 883.51 | 7.45 | 7.45 |
| 791.80 | 7.82 | 7.82 |
| 818.35 | 7.52 | 7.52 |
| 918.34 | 8.63 | 8.63 |
| 841.48 | 7.68 | 7.68 |
| 740.33 | 7.03 | 7.03 |
| 939.75 | 11.38 | 11.38 |
| 794.30 | 7.89 | 7.89 |
| 749.89 | 6.15 | 6.15 |
| 915.15 | 8.69 | 8.69 |
| 866.53 | 7.40 | 7.40 |
| 790.36 | 6.54 | 6.54 |
| 1058.48 | ||
| 882.53 | 6.33 | 6.33 |
| 712.32 | 7.71 | 7.71 |
| 702.88 | 7.26 | 7.26 |
| 980.76 | 11.39 | 11.39 |
| 841.48 | 7.68 | 7.68 |
| 772.68 | 8.29 | 8.29 |
| 814.41 | 6.72 | 6.72 |
| 883.51 | 7.45 | 7.45 |
| 853.67 | 8.76 | 8.76 |
| 896.55 | 7.36 | 7.36 |
| 788.92 | 5.58 | 5.58 |
| 798.41 | 8.13 | 8.13 |
| 820.21 | 7.65 | 7.65 |
| 788.21 | 9.04 | 9.04 |