Basic Information
ID: ALA3888507
Type: Binding
Description: ABAD Enzymatic Activity Assay: The assay for the inhibition of reduction of S-acetoacetyl-CoA (SAAC) by ABAD was carried out with ABAD (418 ng/ml), SAAC (172 μM), NADH (102 μM), and different concentrations of inhibitors (from 0 to 1000 μM) in 93 mM potassium phosphate buffer (pH 7.3). Before the assay, all the assay components except SAAC were pre-incubated for 5 minutes, and the reaction started with the addition of SAAC into the reaction mix. The reaction was carried out for a total 6 minutes at room temperature under steady-state conditions, and the decrease of NADH absorbance at 340 nm was determined every 10 seconds.
Activity Charts
Bioactivity
Activity Types for Assay ALA3888507
Ki
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 394.39 | 4.61 | 4.61 |
| 384.42 | 4.96 | 4.96 |
| 452.43 | 4.39 | 4.39 |
| 440.39 | 4.52 | 4.52 |
| 367.37 | 4.23 | 4.23 |
| 480.48 | 5.17 | 5.17 |
| 394.39 | 4.61 | 4.61 |
| 480.48 | 5.17 | 5.17 |