Basic Information
ID: ALA3888526
Type: Binding
Description: Reductase Activity Assay: The HMGR activity was performed using HMG-CoA reductase assay kit from Sigma-Aldrich with the human recombinant protein or 100 μg total cell lysates from A549 cells. Lovastatin was used as a positive control, and SAHA as a negative control. HMGR activity under defined assay conditions, containing NADPH and HMG-CoA substrate in a final volume of 0.2 mL of 100 mM potassium phosphatate buffer (120 mM KCl, 1 mM EDTA, 5 mM DTT, pH 7.4), were initiated in the presence or absence (control) of test compounds dissolved in dimethylsulfoxide (DMSO). The rates of NADPH consumption were monitored every 20 seconds, for up to 10 minutes, by spectrophotometer at 37° C. and 340 nm.
Cell type: A549
Activity Charts
Bioactivity
Activity Types for Assay ALA3888526
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 404.55 | 4.20 | 4.20 |
| 264.32 | 2.47 | 2.47 |
| 558.65 | 6.31 | 6.31 |
| 451.60 | 3.53 | 3.53 |
| 573.67 | 5.73 | 5.73 |
| 496.56 | 1.82 | 1.82 |
| 437.58 | 3.14 | 3.14 |