Basic Information
ID: ALA3888527
Type: Binding
Description: Fluorescent Activity Assay: The HDAC activity was performed using the HDAC fluorescent activity assay kit (BIOMOL, Plymouth Meeting, Pa., USA) according to the manufacturer's instructions. Briefly, recombinant proteins of HDAC1 or HDAC6 were incubated with test compounds, and HDAC reaction was initiated by addition of Fluor-de-Lys substrate. Samples were incubated for 10 min at room temperature, followed by adding developer to stop the reaction. Fluorescence was measured by fluorometric reader with excitation at 360 nm and emission at 460 nm. The HDAC activity was expressed as arbitrary fluorescence units (AFU).
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3888527
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 404.55 | 4.20 | 4.20 |
| 264.32 | 2.47 | 2.47 |
| 558.65 | 6.31 | 6.31 |
| 451.60 | 3.53 | 3.53 |
| 573.67 | 5.73 | 5.73 |
| 496.56 | 1.82 | 1.82 |
| 437.58 | 3.14 | 3.14 |